Job ID = 1295483 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 25,569,486 reads read : 51,138,972 reads written : 51,138,972 2019-06-03T08:28:50 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:29:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:29:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:30:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:30:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:33:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:33:24 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:35:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:39:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:40:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:41:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:54:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 20,411,953 reads read : 40,823,906 reads written : 40,823,906 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:25:35 45981439 reads; of these: 45981439 (100.00%) were paired; of these: 10251010 (22.29%) aligned concordantly 0 times 25220644 (54.85%) aligned concordantly exactly 1 time 10509785 (22.86%) aligned concordantly >1 times ---- 10251010 pairs aligned concordantly 0 times; of these: 4125372 (40.24%) aligned discordantly 1 time ---- 6125638 pairs aligned 0 times concordantly or discordantly; of these: 12251276 mates make up the pairs; of these: 7751219 (63.27%) aligned 0 times 779887 (6.37%) aligned exactly 1 time 3720170 (30.37%) aligned >1 times 91.57% overall alignment rate Time searching: 02:25:36 Overall time: 02:25:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 13737959 / 39425182 = 0.3485 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 20:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:55:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:55:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:55:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:55:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 20:55:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 20:55:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 20:55:39: 1000000 INFO @ Mon, 03 Jun 2019 20:55:39: 1000000 INFO @ Mon, 03 Jun 2019 20:55:39: 1000000 INFO @ Mon, 03 Jun 2019 20:55:47: 2000000 INFO @ Mon, 03 Jun 2019 20:55:48: 2000000 INFO @ Mon, 03 Jun 2019 20:55:48: 2000000 INFO @ Mon, 03 Jun 2019 20:55:56: 3000000 INFO @ Mon, 03 Jun 2019 20:55:56: 3000000 INFO @ Mon, 03 Jun 2019 20:55:57: 3000000 INFO @ Mon, 03 Jun 2019 20:56:04: 4000000 INFO @ Mon, 03 Jun 2019 20:56:04: 4000000 INFO @ Mon, 03 Jun 2019 20:56:06: 4000000 INFO @ Mon, 03 Jun 2019 20:56:15: 5000000 INFO @ Mon, 03 Jun 2019 20:56:15: 5000000 INFO @ Mon, 03 Jun 2019 20:56:16: 5000000 INFO @ Mon, 03 Jun 2019 20:56:26: 6000000 INFO @ Mon, 03 Jun 2019 20:56:26: 6000000 INFO @ Mon, 03 Jun 2019 20:56:27: 6000000 INFO @ Mon, 03 Jun 2019 20:56:34: 7000000 INFO @ Mon, 03 Jun 2019 20:56:34: 7000000 INFO @ Mon, 03 Jun 2019 20:56:35: 7000000 INFO @ Mon, 03 Jun 2019 20:56:46: 8000000 INFO @ Mon, 03 Jun 2019 20:56:46: 8000000 INFO @ Mon, 03 Jun 2019 20:56:47: 8000000 INFO @ Mon, 03 Jun 2019 20:56:58: 9000000 INFO @ Mon, 03 Jun 2019 20:56:59: 9000000 INFO @ Mon, 03 Jun 2019 20:56:59: 9000000 INFO @ Mon, 03 Jun 2019 20:57:09: 10000000 INFO @ Mon, 03 Jun 2019 20:57:11: 10000000 INFO @ Mon, 03 Jun 2019 20:57:11: 10000000 INFO @ Mon, 03 Jun 2019 20:57:20: 11000000 INFO @ Mon, 03 Jun 2019 20:57:23: 11000000 INFO @ Mon, 03 Jun 2019 20:57:23: 11000000 INFO @ Mon, 03 Jun 2019 20:57:31: 12000000 INFO @ Mon, 03 Jun 2019 20:57:34: 12000000 INFO @ Mon, 03 Jun 2019 20:57:34: 12000000 INFO @ Mon, 03 Jun 2019 20:57:42: 13000000 INFO @ Mon, 03 Jun 2019 20:57:45: 13000000 INFO @ Mon, 03 Jun 2019 20:57:46: 13000000 INFO @ Mon, 03 Jun 2019 20:57:54: 14000000 INFO @ Mon, 03 Jun 2019 20:57:55: 14000000 INFO @ Mon, 03 Jun 2019 20:57:57: 14000000 INFO @ Mon, 03 Jun 2019 20:58:05: 15000000 INFO @ Mon, 03 Jun 2019 20:58:06: 15000000 INFO @ Mon, 03 Jun 2019 20:58:08: 15000000 INFO @ Mon, 03 Jun 2019 20:58:16: 16000000 INFO @ Mon, 03 Jun 2019 20:58:17: 16000000 INFO @ Mon, 03 Jun 2019 20:58:19: 16000000 INFO @ Mon, 03 Jun 2019 20:58:27: 17000000 INFO @ Mon, 03 Jun 2019 20:58:28: 17000000 INFO @ Mon, 03 Jun 2019 20:58:30: 17000000 INFO @ Mon, 03 Jun 2019 20:58:37: 18000000 INFO @ Mon, 03 Jun 2019 20:58:39: 18000000 INFO @ Mon, 03 Jun 2019 20:58:40: 18000000 INFO @ Mon, 03 Jun 2019 20:58:48: 19000000 INFO @ Mon, 03 Jun 2019 20:58:50: 19000000 INFO @ Mon, 03 Jun 2019 20:58:51: 19000000 INFO @ Mon, 03 Jun 2019 20:58:59: 20000000 INFO @ Mon, 03 Jun 2019 20:59:00: 20000000 INFO @ Mon, 03 Jun 2019 20:59:02: 20000000 INFO @ Mon, 03 Jun 2019 20:59:09: 21000000 INFO @ Mon, 03 Jun 2019 20:59:10: 21000000 INFO @ Mon, 03 Jun 2019 20:59:12: 21000000 INFO @ Mon, 03 Jun 2019 20:59:20: 22000000 INFO @ Mon, 03 Jun 2019 20:59:20: 22000000 INFO @ Mon, 03 Jun 2019 20:59:23: 22000000 INFO @ Mon, 03 Jun 2019 20:59:31: 23000000 INFO @ Mon, 03 Jun 2019 20:59:32: 23000000 INFO @ Mon, 03 Jun 2019 20:59:34: 23000000 INFO @ Mon, 03 Jun 2019 20:59:41: 24000000 INFO @ Mon, 03 Jun 2019 20:59:43: 24000000 INFO @ Mon, 03 Jun 2019 20:59:45: 24000000 INFO @ Mon, 03 Jun 2019 20:59:51: 25000000 INFO @ Mon, 03 Jun 2019 20:59:52: 25000000 INFO @ Mon, 03 Jun 2019 20:59:56: 25000000 INFO @ Mon, 03 Jun 2019 21:00:01: 26000000 INFO @ Mon, 03 Jun 2019 21:00:02: 26000000 INFO @ Mon, 03 Jun 2019 21:00:07: 26000000 INFO @ Mon, 03 Jun 2019 21:00:13: 27000000 INFO @ Mon, 03 Jun 2019 21:00:13: 27000000 INFO @ Mon, 03 Jun 2019 21:00:18: 27000000 INFO @ Mon, 03 Jun 2019 21:00:24: 28000000 INFO @ Mon, 03 Jun 2019 21:00:24: 28000000 INFO @ Mon, 03 Jun 2019 21:00:29: 28000000 INFO @ Mon, 03 Jun 2019 21:00:35: 29000000 INFO @ Mon, 03 Jun 2019 21:00:35: 29000000 INFO @ Mon, 03 Jun 2019 21:00:39: 29000000 INFO @ Mon, 03 Jun 2019 21:00:46: 30000000 INFO @ Mon, 03 Jun 2019 21:00:46: 30000000 INFO @ Mon, 03 Jun 2019 21:00:50: 30000000 INFO @ Mon, 03 Jun 2019 21:00:57: 31000000 INFO @ Mon, 03 Jun 2019 21:00:58: 31000000 INFO @ Mon, 03 Jun 2019 21:01:01: 31000000 INFO @ Mon, 03 Jun 2019 21:01:07: 32000000 INFO @ Mon, 03 Jun 2019 21:01:10: 32000000 INFO @ Mon, 03 Jun 2019 21:01:13: 32000000 INFO @ Mon, 03 Jun 2019 21:01:17: 33000000 INFO @ Mon, 03 Jun 2019 21:01:21: 33000000 INFO @ Mon, 03 Jun 2019 21:01:24: 33000000 INFO @ Mon, 03 Jun 2019 21:01:27: 34000000 INFO @ Mon, 03 Jun 2019 21:01:30: 34000000 INFO @ Mon, 03 Jun 2019 21:01:34: 34000000 INFO @ Mon, 03 Jun 2019 21:01:37: 35000000 INFO @ Mon, 03 Jun 2019 21:01:38: 35000000 INFO @ Mon, 03 Jun 2019 21:01:43: 35000000 INFO @ Mon, 03 Jun 2019 21:01:47: 36000000 INFO @ Mon, 03 Jun 2019 21:01:47: 36000000 INFO @ Mon, 03 Jun 2019 21:01:53: 36000000 INFO @ Mon, 03 Jun 2019 21:01:56: 37000000 INFO @ Mon, 03 Jun 2019 21:01:57: 37000000 INFO @ Mon, 03 Jun 2019 21:02:02: 37000000 INFO @ Mon, 03 Jun 2019 21:02:05: 38000000 INFO @ Mon, 03 Jun 2019 21:02:07: 38000000 INFO @ Mon, 03 Jun 2019 21:02:12: 38000000 INFO @ Mon, 03 Jun 2019 21:02:13: 39000000 INFO @ Mon, 03 Jun 2019 21:02:16: 39000000 INFO @ Mon, 03 Jun 2019 21:02:21: 40000000 INFO @ Mon, 03 Jun 2019 21:02:21: 39000000 INFO @ Mon, 03 Jun 2019 21:02:26: 40000000 INFO @ Mon, 03 Jun 2019 21:02:29: 41000000 INFO @ Mon, 03 Jun 2019 21:02:30: 40000000 INFO @ Mon, 03 Jun 2019 21:02:36: 41000000 INFO @ Mon, 03 Jun 2019 21:02:37: 42000000 INFO @ Mon, 03 Jun 2019 21:02:40: 41000000 INFO @ Mon, 03 Jun 2019 21:02:45: 42000000 INFO @ Mon, 03 Jun 2019 21:02:46: 43000000 INFO @ Mon, 03 Jun 2019 21:02:49: 42000000 INFO @ Mon, 03 Jun 2019 21:02:54: 44000000 INFO @ Mon, 03 Jun 2019 21:02:55: 43000000 INFO @ Mon, 03 Jun 2019 21:02:58: 43000000 INFO @ Mon, 03 Jun 2019 21:03:03: 45000000 INFO @ Mon, 03 Jun 2019 21:03:05: 44000000 INFO @ Mon, 03 Jun 2019 21:03:08: 44000000 INFO @ Mon, 03 Jun 2019 21:03:11: 46000000 INFO @ Mon, 03 Jun 2019 21:03:15: 45000000 INFO @ Mon, 03 Jun 2019 21:03:18: 45000000 INFO @ Mon, 03 Jun 2019 21:03:20: 47000000 INFO @ Mon, 03 Jun 2019 21:03:25: 46000000 INFO @ Mon, 03 Jun 2019 21:03:28: 46000000 INFO @ Mon, 03 Jun 2019 21:03:28: 48000000 INFO @ Mon, 03 Jun 2019 21:03:35: 47000000 INFO @ Mon, 03 Jun 2019 21:03:37: 49000000 INFO @ Mon, 03 Jun 2019 21:03:37: 47000000 INFO @ Mon, 03 Jun 2019 21:03:45: 48000000 INFO @ Mon, 03 Jun 2019 21:03:45: 50000000 INFO @ Mon, 03 Jun 2019 21:03:46: 48000000 INFO @ Mon, 03 Jun 2019 21:03:54: 51000000 INFO @ Mon, 03 Jun 2019 21:03:55: 49000000 INFO @ Mon, 03 Jun 2019 21:03:56: 49000000 INFO @ Mon, 03 Jun 2019 21:04:02: 52000000 INFO @ Mon, 03 Jun 2019 21:04:05: 50000000 INFO @ Mon, 03 Jun 2019 21:04:05: 50000000 INFO @ Mon, 03 Jun 2019 21:04:10: 53000000 INFO @ Mon, 03 Jun 2019 21:04:15: 51000000 INFO @ Mon, 03 Jun 2019 21:04:15: 51000000 INFO @ Mon, 03 Jun 2019 21:04:19: 54000000 INFO @ Mon, 03 Jun 2019 21:04:24: 52000000 INFO @ Mon, 03 Jun 2019 21:04:25: 52000000 INFO @ Mon, 03 Jun 2019 21:04:27: 55000000 INFO @ Mon, 03 Jun 2019 21:04:34: 53000000 INFO @ Mon, 03 Jun 2019 21:04:35: 53000000 INFO @ Mon, 03 Jun 2019 21:04:35: 56000000 INFO @ Mon, 03 Jun 2019 21:04:42: #1 tag size is determined as 120 bps INFO @ Mon, 03 Jun 2019 21:04:42: #1 tag size = 120 INFO @ Mon, 03 Jun 2019 21:04:42: #1 total tags in treatment: 22840936 INFO @ Mon, 03 Jun 2019 21:04:42: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:04:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:04:43: #1 tags after filtering in treatment: 15912759 INFO @ Mon, 03 Jun 2019 21:04:43: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 21:04:43: #1 finished! INFO @ Mon, 03 Jun 2019 21:04:43: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:04:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:04:44: 54000000 INFO @ Mon, 03 Jun 2019 21:04:44: #2 number of paired peaks: 2826 INFO @ Mon, 03 Jun 2019 21:04:44: start model_add_line... INFO @ Mon, 03 Jun 2019 21:04:44: 54000000 INFO @ Mon, 03 Jun 2019 21:04:45: start X-correlation... INFO @ Mon, 03 Jun 2019 21:04:45: end of X-cor INFO @ Mon, 03 Jun 2019 21:04:45: #2 finished! INFO @ Mon, 03 Jun 2019 21:04:45: #2 predicted fragment length is 127 bps INFO @ Mon, 03 Jun 2019 21:04:45: #2 alternative fragment length(s) may be 127 bps INFO @ Mon, 03 Jun 2019 21:04:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.10_model.r WARNING @ Mon, 03 Jun 2019 21:04:45: #2 Since the d (127) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 21:04:45: #2 You may need to consider one of the other alternative d(s): 127 WARNING @ Mon, 03 Jun 2019 21:04:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 21:04:45: #3 Call peaks... INFO @ Mon, 03 Jun 2019 21:04:45: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 21:04:53: 55000000 INFO @ Mon, 03 Jun 2019 21:04:54: 55000000 INFO @ Mon, 03 Jun 2019 21:05:02: 56000000 INFO @ Mon, 03 Jun 2019 21:05:04: 56000000 INFO @ Mon, 03 Jun 2019 21:05:09: #1 tag size is determined as 120 bps INFO @ Mon, 03 Jun 2019 21:05:09: #1 tag size = 120 INFO @ Mon, 03 Jun 2019 21:05:09: #1 total tags in treatment: 22840936 INFO @ Mon, 03 Jun 2019 21:05:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:05:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:05:10: #1 tags after filtering in treatment: 15912759 INFO @ Mon, 03 Jun 2019 21:05:10: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 21:05:10: #1 finished! INFO @ Mon, 03 Jun 2019 21:05:10: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:05:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:05:11: #1 tag size is determined as 120 bps INFO @ Mon, 03 Jun 2019 21:05:11: #1 tag size = 120 INFO @ Mon, 03 Jun 2019 21:05:11: #1 total tags in treatment: 22840936 INFO @ Mon, 03 Jun 2019 21:05:11: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:05:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:05:11: #1 tags after filtering in treatment: 15912759 INFO @ Mon, 03 Jun 2019 21:05:11: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 21:05:11: #1 finished! INFO @ Mon, 03 Jun 2019 21:05:11: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:05:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:05:12: #2 number of paired peaks: 2826 INFO @ Mon, 03 Jun 2019 21:05:12: start model_add_line... INFO @ Mon, 03 Jun 2019 21:05:12: start X-correlation... INFO @ Mon, 03 Jun 2019 21:05:12: end of X-cor INFO @ Mon, 03 Jun 2019 21:05:12: #2 finished! INFO @ Mon, 03 Jun 2019 21:05:12: #2 predicted fragment length is 127 bps INFO @ Mon, 03 Jun 2019 21:05:12: #2 alternative fragment length(s) may be 127 bps INFO @ Mon, 03 Jun 2019 21:05:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.05_model.r WARNING @ Mon, 03 Jun 2019 21:05:12: #2 Since the d (127) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 21:05:12: #2 You may need to consider one of the other alternative d(s): 127 WARNING @ Mon, 03 Jun 2019 21:05:12: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 21:05:12: #3 Call peaks... INFO @ Mon, 03 Jun 2019 21:05:12: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 21:05:13: #2 number of paired peaks: 2826 INFO @ Mon, 03 Jun 2019 21:05:13: start model_add_line... INFO @ Mon, 03 Jun 2019 21:05:13: start X-correlation... INFO @ Mon, 03 Jun 2019 21:05:13: end of X-cor INFO @ Mon, 03 Jun 2019 21:05:13: #2 finished! INFO @ Mon, 03 Jun 2019 21:05:13: #2 predicted fragment length is 127 bps INFO @ Mon, 03 Jun 2019 21:05:13: #2 alternative fragment length(s) may be 127 bps INFO @ Mon, 03 Jun 2019 21:05:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.20_model.r WARNING @ Mon, 03 Jun 2019 21:05:14: #2 Since the d (127) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 21:05:14: #2 You may need to consider one of the other alternative d(s): 127 WARNING @ Mon, 03 Jun 2019 21:05:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 21:05:14: #3 Call peaks... INFO @ Mon, 03 Jun 2019 21:05:14: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 21:05:30: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 21:05:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.10_peaks.xls INFO @ Mon, 03 Jun 2019 21:05:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 21:05:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.10_summits.bed INFO @ Mon, 03 Jun 2019 21:05:53: Done! pass1 - making usageList (15 chroms): 6 millis pass2 - checking and writing primary data (11345 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:05:57: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 21:05:59: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 21:06:20: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.05_peaks.xls INFO @ Mon, 03 Jun 2019 21:06:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 21:06:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.05_summits.bed INFO @ Mon, 03 Jun 2019 21:06:20: Done! pass1 - making usageList (15 chroms): 5 millis pass2 - checking and writing primary data (14972 records, 4 fields): 19 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:06:21: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.20_peaks.xls INFO @ Mon, 03 Jun 2019 21:06:21: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 21:06:21: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3404057/SRX3404057.20_summits.bed INFO @ Mon, 03 Jun 2019 21:06:21: Done! pass1 - making usageList (15 chroms): 4 millis pass2 - checking and writing primary data (7198 records, 4 fields): 13 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。