Job ID = 1295426 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 42,731,198 reads read : 85,462,396 reads written : 85,462,396 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:15:19 42731198 reads; of these: 42731198 (100.00%) were paired; of these: 3565007 (8.34%) aligned concordantly 0 times 21134214 (49.46%) aligned concordantly exactly 1 time 18031977 (42.20%) aligned concordantly >1 times ---- 3565007 pairs aligned concordantly 0 times; of these: 127878 (3.59%) aligned discordantly 1 time ---- 3437129 pairs aligned 0 times concordantly or discordantly; of these: 6874258 mates make up the pairs; of these: 4834679 (70.33%) aligned 0 times 1023664 (14.89%) aligned exactly 1 time 1015915 (14.78%) aligned >1 times 94.34% overall alignment rate Time searching: 02:15:19 Overall time: 02:15:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 5867998 / 39131597 = 0.1500 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 16:46:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:46:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:46:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:46:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:46:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:46:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:46:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 16:46:43: #1 read tag files... INFO @ Mon, 03 Jun 2019 16:46:43: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 16:46:51: 1000000 INFO @ Mon, 03 Jun 2019 16:46:53: 1000000 INFO @ Mon, 03 Jun 2019 16:46:53: 1000000 INFO @ Mon, 03 Jun 2019 16:46:59: 2000000 INFO @ Mon, 03 Jun 2019 16:47:03: 2000000 INFO @ Mon, 03 Jun 2019 16:47:03: 2000000 INFO @ Mon, 03 Jun 2019 16:47:06: 3000000 INFO @ Mon, 03 Jun 2019 16:47:13: 3000000 INFO @ Mon, 03 Jun 2019 16:47:13: 3000000 INFO @ Mon, 03 Jun 2019 16:47:14: 4000000 INFO @ Mon, 03 Jun 2019 16:47:21: 5000000 INFO @ Mon, 03 Jun 2019 16:47:23: 4000000 INFO @ Mon, 03 Jun 2019 16:47:23: 4000000 INFO @ Mon, 03 Jun 2019 16:47:29: 6000000 INFO @ Mon, 03 Jun 2019 16:47:33: 5000000 INFO @ Mon, 03 Jun 2019 16:47:34: 5000000 INFO @ Mon, 03 Jun 2019 16:47:37: 7000000 INFO @ Mon, 03 Jun 2019 16:47:43: 6000000 INFO @ Mon, 03 Jun 2019 16:47:44: 6000000 INFO @ Mon, 03 Jun 2019 16:47:44: 8000000 INFO @ Mon, 03 Jun 2019 16:47:52: 9000000 INFO @ Mon, 03 Jun 2019 16:47:53: 7000000 INFO @ Mon, 03 Jun 2019 16:47:53: 7000000 INFO @ Mon, 03 Jun 2019 16:48:00: 10000000 INFO @ Mon, 03 Jun 2019 16:48:03: 8000000 INFO @ Mon, 03 Jun 2019 16:48:03: 8000000 INFO @ Mon, 03 Jun 2019 16:48:07: 11000000 INFO @ Mon, 03 Jun 2019 16:48:13: 9000000 INFO @ Mon, 03 Jun 2019 16:48:13: 9000000 INFO @ Mon, 03 Jun 2019 16:48:15: 12000000 INFO @ Mon, 03 Jun 2019 16:48:22: 13000000 INFO @ Mon, 03 Jun 2019 16:48:23: 10000000 INFO @ Mon, 03 Jun 2019 16:48:23: 10000000 INFO @ Mon, 03 Jun 2019 16:48:30: 14000000 INFO @ Mon, 03 Jun 2019 16:48:33: 11000000 INFO @ Mon, 03 Jun 2019 16:48:33: 11000000 INFO @ Mon, 03 Jun 2019 16:48:38: 15000000 INFO @ Mon, 03 Jun 2019 16:48:43: 12000000 INFO @ Mon, 03 Jun 2019 16:48:43: 12000000 INFO @ Mon, 03 Jun 2019 16:48:45: 16000000 INFO @ Mon, 03 Jun 2019 16:48:53: 13000000 INFO @ Mon, 03 Jun 2019 16:48:53: 17000000 INFO @ Mon, 03 Jun 2019 16:48:53: 13000000 INFO @ Mon, 03 Jun 2019 16:49:01: 18000000 INFO @ Mon, 03 Jun 2019 16:49:03: 14000000 INFO @ Mon, 03 Jun 2019 16:49:03: 14000000 INFO @ Mon, 03 Jun 2019 16:49:08: 19000000 INFO @ Mon, 03 Jun 2019 16:49:13: 15000000 INFO @ Mon, 03 Jun 2019 16:49:14: 15000000 INFO @ Mon, 03 Jun 2019 16:49:16: 20000000 INFO @ Mon, 03 Jun 2019 16:49:23: 16000000 INFO @ Mon, 03 Jun 2019 16:49:23: 21000000 INFO @ Mon, 03 Jun 2019 16:49:23: 16000000 INFO @ Mon, 03 Jun 2019 16:49:31: 22000000 INFO @ Mon, 03 Jun 2019 16:49:33: 17000000 INFO @ Mon, 03 Jun 2019 16:49:34: 17000000 INFO @ Mon, 03 Jun 2019 16:49:38: 23000000 INFO @ Mon, 03 Jun 2019 16:49:44: 18000000 INFO @ Mon, 03 Jun 2019 16:49:45: 18000000 INFO @ Mon, 03 Jun 2019 16:49:45: 24000000 INFO @ Mon, 03 Jun 2019 16:49:53: 25000000 INFO @ Mon, 03 Jun 2019 16:49:55: 19000000 INFO @ Mon, 03 Jun 2019 16:49:55: 19000000 INFO @ Mon, 03 Jun 2019 16:50:00: 26000000 INFO @ Mon, 03 Jun 2019 16:50:05: 20000000 INFO @ Mon, 03 Jun 2019 16:50:05: 20000000 INFO @ Mon, 03 Jun 2019 16:50:08: 27000000 INFO @ Mon, 03 Jun 2019 16:50:15: 21000000 INFO @ Mon, 03 Jun 2019 16:50:15: 21000000 INFO @ Mon, 03 Jun 2019 16:50:16: 28000000 INFO @ Mon, 03 Jun 2019 16:50:23: 29000000 INFO @ Mon, 03 Jun 2019 16:50:24: 22000000 INFO @ Mon, 03 Jun 2019 16:50:25: 22000000 INFO @ Mon, 03 Jun 2019 16:50:30: 30000000 INFO @ Mon, 03 Jun 2019 16:50:34: 23000000 INFO @ Mon, 03 Jun 2019 16:50:35: 23000000 INFO @ Mon, 03 Jun 2019 16:50:38: 31000000 INFO @ Mon, 03 Jun 2019 16:50:44: 24000000 INFO @ Mon, 03 Jun 2019 16:50:44: 24000000 INFO @ Mon, 03 Jun 2019 16:50:45: 32000000 INFO @ Mon, 03 Jun 2019 16:50:53: 33000000 INFO @ Mon, 03 Jun 2019 16:50:53: 25000000 INFO @ Mon, 03 Jun 2019 16:50:54: 25000000 INFO @ Mon, 03 Jun 2019 16:51:00: 34000000 INFO @ Mon, 03 Jun 2019 16:51:03: 26000000 INFO @ Mon, 03 Jun 2019 16:51:04: 26000000 INFO @ Mon, 03 Jun 2019 16:51:08: 35000000 INFO @ Mon, 03 Jun 2019 16:51:13: 27000000 INFO @ Mon, 03 Jun 2019 16:51:13: 27000000 INFO @ Mon, 03 Jun 2019 16:51:15: 36000000 INFO @ Mon, 03 Jun 2019 16:51:23: 28000000 INFO @ Mon, 03 Jun 2019 16:51:23: 37000000 INFO @ Mon, 03 Jun 2019 16:51:23: 28000000 INFO @ Mon, 03 Jun 2019 16:51:30: 38000000 INFO @ Mon, 03 Jun 2019 16:51:32: 29000000 INFO @ Mon, 03 Jun 2019 16:51:33: 29000000 INFO @ Mon, 03 Jun 2019 16:51:38: 39000000 INFO @ Mon, 03 Jun 2019 16:51:42: 30000000 INFO @ Mon, 03 Jun 2019 16:51:43: 30000000 INFO @ Mon, 03 Jun 2019 16:51:45: 40000000 INFO @ Mon, 03 Jun 2019 16:51:52: 31000000 INFO @ Mon, 03 Jun 2019 16:51:52: 41000000 INFO @ Mon, 03 Jun 2019 16:51:53: 31000000 INFO @ Mon, 03 Jun 2019 16:52:00: 42000000 INFO @ Mon, 03 Jun 2019 16:52:02: 32000000 INFO @ Mon, 03 Jun 2019 16:52:02: 32000000 INFO @ Mon, 03 Jun 2019 16:52:08: 43000000 INFO @ Mon, 03 Jun 2019 16:52:12: 33000000 INFO @ Mon, 03 Jun 2019 16:52:12: 33000000 INFO @ Mon, 03 Jun 2019 16:52:15: 44000000 INFO @ Mon, 03 Jun 2019 16:52:22: 34000000 INFO @ Mon, 03 Jun 2019 16:52:23: 45000000 INFO @ Mon, 03 Jun 2019 16:52:23: 34000000 INFO @ Mon, 03 Jun 2019 16:52:30: 46000000 INFO @ Mon, 03 Jun 2019 16:52:33: 35000000 INFO @ Mon, 03 Jun 2019 16:52:33: 35000000 INFO @ Mon, 03 Jun 2019 16:52:38: 47000000 INFO @ Mon, 03 Jun 2019 16:52:43: 36000000 INFO @ Mon, 03 Jun 2019 16:52:43: 36000000 INFO @ Mon, 03 Jun 2019 16:52:45: 48000000 INFO @ Mon, 03 Jun 2019 16:52:53: 49000000 INFO @ Mon, 03 Jun 2019 16:52:53: 37000000 INFO @ Mon, 03 Jun 2019 16:52:53: 37000000 INFO @ Mon, 03 Jun 2019 16:53:00: 50000000 INFO @ Mon, 03 Jun 2019 16:53:02: 38000000 INFO @ Mon, 03 Jun 2019 16:53:03: 38000000 INFO @ Mon, 03 Jun 2019 16:53:08: 51000000 INFO @ Mon, 03 Jun 2019 16:53:12: 39000000 INFO @ Mon, 03 Jun 2019 16:53:13: 39000000 INFO @ Mon, 03 Jun 2019 16:53:15: 52000000 INFO @ Mon, 03 Jun 2019 16:53:22: 40000000 INFO @ Mon, 03 Jun 2019 16:53:23: 53000000 INFO @ Mon, 03 Jun 2019 16:53:23: 40000000 INFO @ Mon, 03 Jun 2019 16:53:30: 54000000 INFO @ Mon, 03 Jun 2019 16:53:32: 41000000 INFO @ Mon, 03 Jun 2019 16:53:32: 41000000 INFO @ Mon, 03 Jun 2019 16:53:37: 55000000 INFO @ Mon, 03 Jun 2019 16:53:42: 42000000 INFO @ Mon, 03 Jun 2019 16:53:42: 42000000 INFO @ Mon, 03 Jun 2019 16:53:44: 56000000 INFO @ Mon, 03 Jun 2019 16:53:52: 43000000 INFO @ Mon, 03 Jun 2019 16:53:52: 57000000 INFO @ Mon, 03 Jun 2019 16:53:52: 43000000 INFO @ Mon, 03 Jun 2019 16:53:59: 58000000 INFO @ Mon, 03 Jun 2019 16:54:01: 44000000 INFO @ Mon, 03 Jun 2019 16:54:02: 44000000 INFO @ Mon, 03 Jun 2019 16:54:06: 59000000 INFO @ Mon, 03 Jun 2019 16:54:11: 45000000 INFO @ Mon, 03 Jun 2019 16:54:12: 45000000 INFO @ Mon, 03 Jun 2019 16:54:13: 60000000 INFO @ Mon, 03 Jun 2019 16:54:20: 61000000 INFO @ Mon, 03 Jun 2019 16:54:21: 46000000 INFO @ Mon, 03 Jun 2019 16:54:21: 46000000 INFO @ Mon, 03 Jun 2019 16:54:28: 62000000 INFO @ Mon, 03 Jun 2019 16:54:31: 47000000 INFO @ Mon, 03 Jun 2019 16:54:31: 47000000 INFO @ Mon, 03 Jun 2019 16:54:35: 63000000 INFO @ Mon, 03 Jun 2019 16:54:41: 48000000 INFO @ Mon, 03 Jun 2019 16:54:41: 48000000 INFO @ Mon, 03 Jun 2019 16:54:42: 64000000 INFO @ Mon, 03 Jun 2019 16:54:49: 65000000 INFO @ Mon, 03 Jun 2019 16:54:51: 49000000 INFO @ Mon, 03 Jun 2019 16:54:52: 49000000 INFO @ Mon, 03 Jun 2019 16:54:56: 66000000 INFO @ Mon, 03 Jun 2019 16:55:01: 50000000 INFO @ Mon, 03 Jun 2019 16:55:03: 50000000 INFO @ Mon, 03 Jun 2019 16:55:04: 67000000 INFO @ Mon, 03 Jun 2019 16:55:11: 68000000 INFO @ Mon, 03 Jun 2019 16:55:11: 51000000 INFO @ Mon, 03 Jun 2019 16:55:12: 51000000 INFO @ Mon, 03 Jun 2019 16:55:17: #1 tag size is determined as 42 bps INFO @ Mon, 03 Jun 2019 16:55:17: #1 tag size = 42 INFO @ Mon, 03 Jun 2019 16:55:17: #1 total tags in treatment: 33300170 INFO @ Mon, 03 Jun 2019 16:55:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:55:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:55:18: #1 tags after filtering in treatment: 28182481 INFO @ Mon, 03 Jun 2019 16:55:18: #1 Redundant rate of treatment: 0.15 INFO @ Mon, 03 Jun 2019 16:55:18: #1 finished! INFO @ Mon, 03 Jun 2019 16:55:18: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:55:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:55:20: 52000000 INFO @ Mon, 03 Jun 2019 16:55:21: #2 number of paired peaks: 738 WARNING @ Mon, 03 Jun 2019 16:55:21: Fewer paired peaks (738) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 738 pairs to build model! INFO @ Mon, 03 Jun 2019 16:55:21: start model_add_line... INFO @ Mon, 03 Jun 2019 16:55:21: start X-correlation... INFO @ Mon, 03 Jun 2019 16:55:21: end of X-cor INFO @ Mon, 03 Jun 2019 16:55:21: #2 finished! INFO @ Mon, 03 Jun 2019 16:55:21: #2 predicted fragment length is 116 bps INFO @ Mon, 03 Jun 2019 16:55:21: #2 alternative fragment length(s) may be 2,116 bps INFO @ Mon, 03 Jun 2019 16:55:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.20_model.r INFO @ Mon, 03 Jun 2019 16:55:21: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:55:21: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:55:22: 52000000 INFO @ Mon, 03 Jun 2019 16:55:30: 53000000 INFO @ Mon, 03 Jun 2019 16:55:31: 53000000 INFO @ Mon, 03 Jun 2019 16:55:39: 54000000 INFO @ Mon, 03 Jun 2019 16:55:40: 54000000 INFO @ Mon, 03 Jun 2019 16:55:49: 55000000 INFO @ Mon, 03 Jun 2019 16:55:50: 55000000 INFO @ Mon, 03 Jun 2019 16:55:58: 56000000 INFO @ Mon, 03 Jun 2019 16:55:59: 56000000 INFO @ Mon, 03 Jun 2019 16:56:07: 57000000 INFO @ Mon, 03 Jun 2019 16:56:09: 57000000 INFO @ Mon, 03 Jun 2019 16:56:17: 58000000 INFO @ Mon, 03 Jun 2019 16:56:18: 58000000 INFO @ Mon, 03 Jun 2019 16:56:26: 59000000 INFO @ Mon, 03 Jun 2019 16:56:27: 59000000 INFO @ Mon, 03 Jun 2019 16:56:31: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:56:35: 60000000 INFO @ Mon, 03 Jun 2019 16:56:36: 60000000 INFO @ Mon, 03 Jun 2019 16:56:44: 61000000 INFO @ Mon, 03 Jun 2019 16:56:45: 61000000 INFO @ Mon, 03 Jun 2019 16:56:53: 62000000 INFO @ Mon, 03 Jun 2019 16:56:54: 62000000 INFO @ Mon, 03 Jun 2019 16:57:02: 63000000 INFO @ Mon, 03 Jun 2019 16:57:03: 63000000 INFO @ Mon, 03 Jun 2019 16:57:03: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.20_peaks.xls INFO @ Mon, 03 Jun 2019 16:57:03: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:57:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.20_summits.bed INFO @ Mon, 03 Jun 2019 16:57:04: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1613 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 16:57:12: 64000000 INFO @ Mon, 03 Jun 2019 16:57:13: 64000000 INFO @ Mon, 03 Jun 2019 16:57:22: 65000000 INFO @ Mon, 03 Jun 2019 16:57:23: 65000000 INFO @ Mon, 03 Jun 2019 16:57:31: 66000000 INFO @ Mon, 03 Jun 2019 16:57:32: 66000000 INFO @ Mon, 03 Jun 2019 16:57:40: 67000000 INFO @ Mon, 03 Jun 2019 16:57:41: 67000000 INFO @ Mon, 03 Jun 2019 16:57:49: 68000000 INFO @ Mon, 03 Jun 2019 16:57:50: 68000000 INFO @ Mon, 03 Jun 2019 16:57:58: #1 tag size is determined as 42 bps INFO @ Mon, 03 Jun 2019 16:57:58: #1 tag size = 42 INFO @ Mon, 03 Jun 2019 16:57:58: #1 total tags in treatment: 33300170 INFO @ Mon, 03 Jun 2019 16:57:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:57:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:57:58: #1 tags after filtering in treatment: 28182481 INFO @ Mon, 03 Jun 2019 16:57:58: #1 Redundant rate of treatment: 0.15 INFO @ Mon, 03 Jun 2019 16:57:58: #1 finished! INFO @ Mon, 03 Jun 2019 16:57:58: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:57:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:57:58: #1 tag size is determined as 42 bps INFO @ Mon, 03 Jun 2019 16:57:58: #1 tag size = 42 INFO @ Mon, 03 Jun 2019 16:57:58: #1 total tags in treatment: 33300170 INFO @ Mon, 03 Jun 2019 16:57:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 16:57:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 16:57:59: #1 tags after filtering in treatment: 28182481 INFO @ Mon, 03 Jun 2019 16:57:59: #1 Redundant rate of treatment: 0.15 INFO @ Mon, 03 Jun 2019 16:57:59: #1 finished! INFO @ Mon, 03 Jun 2019 16:57:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 16:57:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 16:58:01: #2 number of paired peaks: 738 WARNING @ Mon, 03 Jun 2019 16:58:01: Fewer paired peaks (738) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 738 pairs to build model! INFO @ Mon, 03 Jun 2019 16:58:01: start model_add_line... INFO @ Mon, 03 Jun 2019 16:58:01: start X-correlation... INFO @ Mon, 03 Jun 2019 16:58:01: end of X-cor INFO @ Mon, 03 Jun 2019 16:58:01: #2 finished! INFO @ Mon, 03 Jun 2019 16:58:01: #2 predicted fragment length is 116 bps INFO @ Mon, 03 Jun 2019 16:58:01: #2 alternative fragment length(s) may be 2,116 bps INFO @ Mon, 03 Jun 2019 16:58:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.05_model.r INFO @ Mon, 03 Jun 2019 16:58:01: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:58:01: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:58:02: #2 number of paired peaks: 738 WARNING @ Mon, 03 Jun 2019 16:58:02: Fewer paired peaks (738) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 738 pairs to build model! INFO @ Mon, 03 Jun 2019 16:58:02: start model_add_line... INFO @ Mon, 03 Jun 2019 16:58:02: start X-correlation... INFO @ Mon, 03 Jun 2019 16:58:02: end of X-cor INFO @ Mon, 03 Jun 2019 16:58:02: #2 finished! INFO @ Mon, 03 Jun 2019 16:58:02: #2 predicted fragment length is 116 bps INFO @ Mon, 03 Jun 2019 16:58:02: #2 alternative fragment length(s) may be 2,116 bps INFO @ Mon, 03 Jun 2019 16:58:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.10_model.r INFO @ Mon, 03 Jun 2019 16:58:02: #3 Call peaks... INFO @ Mon, 03 Jun 2019 16:58:02: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 16:59:12: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:59:13: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 16:59:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.10_peaks.xls INFO @ Mon, 03 Jun 2019 16:59:45: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.05_peaks.xls INFO @ Mon, 03 Jun 2019 16:59:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:59:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.10_summits.bed INFO @ Mon, 03 Jun 2019 16:59:45: Done! INFO @ Mon, 03 Jun 2019 16:59:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 16:59:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3393646/SRX3393646.05_summits.bed INFO @ Mon, 03 Jun 2019 16:59:45: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (3031 records, 4 fields): 8 millis CompletedMACS2peakCalling pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (6128 records, 4 fields): 14 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。