Job ID = 1295408 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,251,934 reads read : 22,251,934 reads written : 22,251,934 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:02 22251934 reads; of these: 22251934 (100.00%) were unpaired; of these: 832647 (3.74%) aligned 0 times 15008579 (67.45%) aligned exactly 1 time 6410708 (28.81%) aligned >1 times 96.26% overall alignment rate Time searching: 00:10:02 Overall time: 00:10:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5187121 / 21419287 = 0.2422 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:56:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:56:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:56:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:56:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:56:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:56:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:56:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:56:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:56:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:56:48: 1000000 INFO @ Mon, 03 Jun 2019 13:56:48: 1000000 INFO @ Mon, 03 Jun 2019 13:56:49: 1000000 INFO @ Mon, 03 Jun 2019 13:56:55: 2000000 INFO @ Mon, 03 Jun 2019 13:56:58: 2000000 INFO @ Mon, 03 Jun 2019 13:56:59: 2000000 INFO @ Mon, 03 Jun 2019 13:57:04: 3000000 INFO @ Mon, 03 Jun 2019 13:57:07: 3000000 INFO @ Mon, 03 Jun 2019 13:57:09: 3000000 INFO @ Mon, 03 Jun 2019 13:57:11: 4000000 INFO @ Mon, 03 Jun 2019 13:57:15: 4000000 INFO @ Mon, 03 Jun 2019 13:57:17: 4000000 INFO @ Mon, 03 Jun 2019 13:57:19: 5000000 INFO @ Mon, 03 Jun 2019 13:57:22: 5000000 INFO @ Mon, 03 Jun 2019 13:57:27: 5000000 INFO @ Mon, 03 Jun 2019 13:57:27: 6000000 INFO @ Mon, 03 Jun 2019 13:57:30: 6000000 INFO @ Mon, 03 Jun 2019 13:57:34: 7000000 INFO @ Mon, 03 Jun 2019 13:57:36: 6000000 INFO @ Mon, 03 Jun 2019 13:57:38: 7000000 INFO @ Mon, 03 Jun 2019 13:57:42: 8000000 INFO @ Mon, 03 Jun 2019 13:57:45: 7000000 INFO @ Mon, 03 Jun 2019 13:57:46: 8000000 INFO @ Mon, 03 Jun 2019 13:57:49: 9000000 INFO @ Mon, 03 Jun 2019 13:57:53: 9000000 INFO @ Mon, 03 Jun 2019 13:57:54: 8000000 INFO @ Mon, 03 Jun 2019 13:57:57: 10000000 INFO @ Mon, 03 Jun 2019 13:58:01: 10000000 INFO @ Mon, 03 Jun 2019 13:58:04: 9000000 INFO @ Mon, 03 Jun 2019 13:58:05: 11000000 INFO @ Mon, 03 Jun 2019 13:58:09: 11000000 INFO @ Mon, 03 Jun 2019 13:58:12: 12000000 INFO @ Mon, 03 Jun 2019 13:58:12: 10000000 INFO @ Mon, 03 Jun 2019 13:58:17: 12000000 INFO @ Mon, 03 Jun 2019 13:58:20: 13000000 INFO @ Mon, 03 Jun 2019 13:58:21: 11000000 INFO @ Mon, 03 Jun 2019 13:58:24: 13000000 INFO @ Mon, 03 Jun 2019 13:58:28: 14000000 INFO @ Mon, 03 Jun 2019 13:58:30: 12000000 INFO @ Mon, 03 Jun 2019 13:58:32: 14000000 INFO @ Mon, 03 Jun 2019 13:58:35: 15000000 INFO @ Mon, 03 Jun 2019 13:58:38: 13000000 INFO @ Mon, 03 Jun 2019 13:58:39: 15000000 INFO @ Mon, 03 Jun 2019 13:58:42: 16000000 INFO @ Mon, 03 Jun 2019 13:58:44: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 13:58:44: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 13:58:44: #1 total tags in treatment: 16232166 INFO @ Mon, 03 Jun 2019 13:58:44: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:58:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:58:45: #1 tags after filtering in treatment: 16232166 INFO @ Mon, 03 Jun 2019 13:58:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:58:45: #1 finished! INFO @ Mon, 03 Jun 2019 13:58:45: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:58:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:58:46: #2 number of paired peaks: 3 WARNING @ Mon, 03 Jun 2019 13:58:46: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:58:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:58:47: 14000000 INFO @ Mon, 03 Jun 2019 13:58:47: 16000000 INFO @ Mon, 03 Jun 2019 13:58:49: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 13:58:49: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 13:58:49: #1 total tags in treatment: 16232166 INFO @ Mon, 03 Jun 2019 13:58:49: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:58:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:58:49: #1 tags after filtering in treatment: 16232166 INFO @ Mon, 03 Jun 2019 13:58:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:58:49: #1 finished! INFO @ Mon, 03 Jun 2019 13:58:49: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:58:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:58:51: #2 number of paired peaks: 3 WARNING @ Mon, 03 Jun 2019 13:58:51: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:58:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:58:55: 15000000 INFO @ Mon, 03 Jun 2019 13:59:04: 16000000 INFO @ Mon, 03 Jun 2019 13:59:06: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 13:59:06: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 13:59:06: #1 total tags in treatment: 16232166 INFO @ Mon, 03 Jun 2019 13:59:06: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:59:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:59:06: #1 tags after filtering in treatment: 16232166 INFO @ Mon, 03 Jun 2019 13:59:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:59:06: #1 finished! INFO @ Mon, 03 Jun 2019 13:59:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:59:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:59:08: #2 number of paired peaks: 3 WARNING @ Mon, 03 Jun 2019 13:59:08: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:59:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336283/SRX336283.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。