Job ID = 1295375 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,203,362 reads read : 32,203,362 reads written : 32,203,362 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:10 32203362 reads; of these: 32203362 (100.00%) were unpaired; of these: 3172181 (9.85%) aligned 0 times 22508900 (69.90%) aligned exactly 1 time 6522281 (20.25%) aligned >1 times 90.15% overall alignment rate Time searching: 00:10:10 Overall time: 00:10:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7038730 / 29031181 = 0.2425 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:49:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:49:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:49:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:49:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:49:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:49:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:49:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:49:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:49:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:49:55: 1000000 INFO @ Mon, 03 Jun 2019 13:49:57: 1000000 INFO @ Mon, 03 Jun 2019 13:49:57: 1000000 INFO @ Mon, 03 Jun 2019 13:50:02: 2000000 INFO @ Mon, 03 Jun 2019 13:50:06: 2000000 INFO @ Mon, 03 Jun 2019 13:50:06: 2000000 INFO @ Mon, 03 Jun 2019 13:50:09: 3000000 INFO @ Mon, 03 Jun 2019 13:50:15: 3000000 INFO @ Mon, 03 Jun 2019 13:50:15: 3000000 INFO @ Mon, 03 Jun 2019 13:50:16: 4000000 INFO @ Mon, 03 Jun 2019 13:50:23: 5000000 INFO @ Mon, 03 Jun 2019 13:50:24: 4000000 INFO @ Mon, 03 Jun 2019 13:50:25: 4000000 INFO @ Mon, 03 Jun 2019 13:50:29: 6000000 INFO @ Mon, 03 Jun 2019 13:50:33: 5000000 INFO @ Mon, 03 Jun 2019 13:50:34: 5000000 INFO @ Mon, 03 Jun 2019 13:50:36: 7000000 INFO @ Mon, 03 Jun 2019 13:50:42: 6000000 INFO @ Mon, 03 Jun 2019 13:50:42: 6000000 INFO @ Mon, 03 Jun 2019 13:50:43: 8000000 INFO @ Mon, 03 Jun 2019 13:50:50: 9000000 INFO @ Mon, 03 Jun 2019 13:50:51: 7000000 INFO @ Mon, 03 Jun 2019 13:50:51: 7000000 INFO @ Mon, 03 Jun 2019 13:50:57: 10000000 INFO @ Mon, 03 Jun 2019 13:51:00: 8000000 INFO @ Mon, 03 Jun 2019 13:51:00: 8000000 INFO @ Mon, 03 Jun 2019 13:51:04: 11000000 INFO @ Mon, 03 Jun 2019 13:51:09: 9000000 INFO @ Mon, 03 Jun 2019 13:51:09: 9000000 INFO @ Mon, 03 Jun 2019 13:51:11: 12000000 INFO @ Mon, 03 Jun 2019 13:51:18: 10000000 INFO @ Mon, 03 Jun 2019 13:51:18: 13000000 INFO @ Mon, 03 Jun 2019 13:51:18: 10000000 INFO @ Mon, 03 Jun 2019 13:51:24: 14000000 INFO @ Mon, 03 Jun 2019 13:51:26: 11000000 INFO @ Mon, 03 Jun 2019 13:51:27: 11000000 INFO @ Mon, 03 Jun 2019 13:51:31: 15000000 INFO @ Mon, 03 Jun 2019 13:51:35: 12000000 INFO @ Mon, 03 Jun 2019 13:51:35: 12000000 INFO @ Mon, 03 Jun 2019 13:51:38: 16000000 INFO @ Mon, 03 Jun 2019 13:51:44: 13000000 INFO @ Mon, 03 Jun 2019 13:51:44: 13000000 INFO @ Mon, 03 Jun 2019 13:51:45: 17000000 INFO @ Mon, 03 Jun 2019 13:51:52: 18000000 INFO @ Mon, 03 Jun 2019 13:51:53: 14000000 INFO @ Mon, 03 Jun 2019 13:51:53: 14000000 INFO @ Mon, 03 Jun 2019 13:51:59: 19000000 INFO @ Mon, 03 Jun 2019 13:52:01: 15000000 INFO @ Mon, 03 Jun 2019 13:52:02: 15000000 INFO @ Mon, 03 Jun 2019 13:52:06: 20000000 INFO @ Mon, 03 Jun 2019 13:52:10: 16000000 INFO @ Mon, 03 Jun 2019 13:52:11: 16000000 INFO @ Mon, 03 Jun 2019 13:52:13: 21000000 INFO @ Mon, 03 Jun 2019 13:52:19: 17000000 INFO @ Mon, 03 Jun 2019 13:52:20: 17000000 INFO @ Mon, 03 Jun 2019 13:52:20: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:52:20: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:52:20: #1 total tags in treatment: 21992451 INFO @ Mon, 03 Jun 2019 13:52:20: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:52:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:52:21: #1 tags after filtering in treatment: 21992451 INFO @ Mon, 03 Jun 2019 13:52:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:52:21: #1 finished! INFO @ Mon, 03 Jun 2019 13:52:21: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:52:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:52:23: #2 number of paired peaks: 8 WARNING @ Mon, 03 Jun 2019 13:52:23: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:52:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:52:28: 18000000 INFO @ Mon, 03 Jun 2019 13:52:29: 18000000 INFO @ Mon, 03 Jun 2019 13:52:37: 19000000 INFO @ Mon, 03 Jun 2019 13:52:38: 19000000 INFO @ Mon, 03 Jun 2019 13:52:46: 20000000 INFO @ Mon, 03 Jun 2019 13:52:46: 20000000 INFO @ Mon, 03 Jun 2019 13:52:55: 21000000 INFO @ Mon, 03 Jun 2019 13:52:55: 21000000 INFO @ Mon, 03 Jun 2019 13:53:04: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:53:04: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:53:04: #1 total tags in treatment: 21992451 INFO @ Mon, 03 Jun 2019 13:53:04: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:53:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:53:04: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:53:04: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:53:04: #1 total tags in treatment: 21992451 INFO @ Mon, 03 Jun 2019 13:53:04: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:53:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:53:05: #1 tags after filtering in treatment: 21992451 INFO @ Mon, 03 Jun 2019 13:53:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:53:05: #1 finished! INFO @ Mon, 03 Jun 2019 13:53:05: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:53:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:53:05: #1 tags after filtering in treatment: 21992451 INFO @ Mon, 03 Jun 2019 13:53:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:53:05: #1 finished! INFO @ Mon, 03 Jun 2019 13:53:05: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:53:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:53:06: #2 number of paired peaks: 8 WARNING @ Mon, 03 Jun 2019 13:53:06: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:53:06: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 13:53:07: #2 number of paired peaks: 8 WARNING @ Mon, 03 Jun 2019 13:53:07: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:53:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis cut: /home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.20_peaks.narrowPeak: No such file or directory needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335510/SRX335510.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。