Job ID = 1295369 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 36,182,857 reads read : 36,182,857 reads written : 36,182,857 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:37 36182857 reads; of these: 36182857 (100.00%) were unpaired; of these: 22696764 (62.73%) aligned 0 times 9830481 (27.17%) aligned exactly 1 time 3655612 (10.10%) aligned >1 times 37.27% overall alignment rate Time searching: 00:07:37 Overall time: 00:07:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2449770 / 13486093 = 0.1817 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:41:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:41:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:41:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:41:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:41:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:41:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:41:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:41:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:41:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:41:57: 1000000 INFO @ Mon, 03 Jun 2019 13:41:57: 1000000 INFO @ Mon, 03 Jun 2019 13:41:58: 1000000 INFO @ Mon, 03 Jun 2019 13:42:04: 2000000 INFO @ Mon, 03 Jun 2019 13:42:04: 2000000 INFO @ Mon, 03 Jun 2019 13:42:05: 2000000 INFO @ Mon, 03 Jun 2019 13:42:11: 3000000 INFO @ Mon, 03 Jun 2019 13:42:11: 3000000 INFO @ Mon, 03 Jun 2019 13:42:13: 3000000 INFO @ Mon, 03 Jun 2019 13:42:18: 4000000 INFO @ Mon, 03 Jun 2019 13:42:18: 4000000 INFO @ Mon, 03 Jun 2019 13:42:21: 4000000 INFO @ Mon, 03 Jun 2019 13:42:25: 5000000 INFO @ Mon, 03 Jun 2019 13:42:25: 5000000 INFO @ Mon, 03 Jun 2019 13:42:28: 5000000 INFO @ Mon, 03 Jun 2019 13:42:32: 6000000 INFO @ Mon, 03 Jun 2019 13:42:32: 6000000 INFO @ Mon, 03 Jun 2019 13:42:37: 6000000 INFO @ Mon, 03 Jun 2019 13:42:39: 7000000 INFO @ Mon, 03 Jun 2019 13:42:39: 7000000 INFO @ Mon, 03 Jun 2019 13:42:45: 7000000 INFO @ Mon, 03 Jun 2019 13:42:46: 8000000 INFO @ Mon, 03 Jun 2019 13:42:46: 8000000 INFO @ Mon, 03 Jun 2019 13:42:53: 8000000 INFO @ Mon, 03 Jun 2019 13:42:53: 9000000 INFO @ Mon, 03 Jun 2019 13:42:53: 9000000 INFO @ Mon, 03 Jun 2019 13:43:00: 10000000 INFO @ Mon, 03 Jun 2019 13:43:00: 10000000 INFO @ Mon, 03 Jun 2019 13:43:01: 9000000 INFO @ Mon, 03 Jun 2019 13:43:07: 11000000 INFO @ Mon, 03 Jun 2019 13:43:07: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:43:07: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:43:07: #1 total tags in treatment: 11036323 INFO @ Mon, 03 Jun 2019 13:43:07: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:43:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:43:07: 11000000 INFO @ Mon, 03 Jun 2019 13:43:07: #1 tags after filtering in treatment: 11036323 INFO @ Mon, 03 Jun 2019 13:43:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:43:07: #1 finished! INFO @ Mon, 03 Jun 2019 13:43:07: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:43:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:43:08: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:43:08: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:43:08: #1 total tags in treatment: 11036323 INFO @ Mon, 03 Jun 2019 13:43:08: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:43:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:43:08: #1 tags after filtering in treatment: 11036323 INFO @ Mon, 03 Jun 2019 13:43:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:43:08: #1 finished! INFO @ Mon, 03 Jun 2019 13:43:08: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:43:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:43:08: #2 number of paired peaks: 64 WARNING @ Mon, 03 Jun 2019 13:43:08: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:43:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:43:09: 10000000 INFO @ Mon, 03 Jun 2019 13:43:09: #2 number of paired peaks: 64 WARNING @ Mon, 03 Jun 2019 13:43:09: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:43:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:43:17: 11000000 INFO @ Mon, 03 Jun 2019 13:43:17: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:43:17: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:43:17: #1 total tags in treatment: 11036323 INFO @ Mon, 03 Jun 2019 13:43:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:43:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:43:17: #1 tags after filtering in treatment: 11036323 INFO @ Mon, 03 Jun 2019 13:43:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:43:17: #1 finished! INFO @ Mon, 03 Jun 2019 13:43:17: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:43:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:43:18: #2 number of paired peaks: 64 WARNING @ Mon, 03 Jun 2019 13:43:18: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:43:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335506/SRX335506.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。