Job ID = 1295368 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 33,349,222 reads read : 33,349,222 reads written : 33,349,222 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:19 33349222 reads; of these: 33349222 (100.00%) were unpaired; of these: 4936553 (14.80%) aligned 0 times 22867051 (68.57%) aligned exactly 1 time 5545618 (16.63%) aligned >1 times 85.20% overall alignment rate Time searching: 00:11:20 Overall time: 00:11:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5662997 / 28412669 = 0.1993 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:49:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:49:12: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:49:12: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:49:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:49:12: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:49:12: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:49:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:49:12: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:49:12: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:49:22: 1000000 INFO @ Mon, 03 Jun 2019 13:49:23: 1000000 INFO @ Mon, 03 Jun 2019 13:49:23: 1000000 INFO @ Mon, 03 Jun 2019 13:49:30: 2000000 INFO @ Mon, 03 Jun 2019 13:49:33: 2000000 INFO @ Mon, 03 Jun 2019 13:49:33: 2000000 INFO @ Mon, 03 Jun 2019 13:49:39: 3000000 INFO @ Mon, 03 Jun 2019 13:49:43: 3000000 INFO @ Mon, 03 Jun 2019 13:49:43: 3000000 INFO @ Mon, 03 Jun 2019 13:49:47: 4000000 INFO @ Mon, 03 Jun 2019 13:49:53: 4000000 INFO @ Mon, 03 Jun 2019 13:49:53: 4000000 INFO @ Mon, 03 Jun 2019 13:49:56: 5000000 INFO @ Mon, 03 Jun 2019 13:50:03: 5000000 INFO @ Mon, 03 Jun 2019 13:50:03: 5000000 INFO @ Mon, 03 Jun 2019 13:50:04: 6000000 INFO @ Mon, 03 Jun 2019 13:50:13: 7000000 INFO @ Mon, 03 Jun 2019 13:50:13: 6000000 INFO @ Mon, 03 Jun 2019 13:50:14: 6000000 INFO @ Mon, 03 Jun 2019 13:50:21: 8000000 INFO @ Mon, 03 Jun 2019 13:50:22: 7000000 INFO @ Mon, 03 Jun 2019 13:50:24: 7000000 INFO @ Mon, 03 Jun 2019 13:50:30: 9000000 INFO @ Mon, 03 Jun 2019 13:50:32: 8000000 INFO @ Mon, 03 Jun 2019 13:50:34: 8000000 INFO @ Mon, 03 Jun 2019 13:50:38: 10000000 INFO @ Mon, 03 Jun 2019 13:50:41: 9000000 INFO @ Mon, 03 Jun 2019 13:50:44: 9000000 INFO @ Mon, 03 Jun 2019 13:50:47: 11000000 INFO @ Mon, 03 Jun 2019 13:50:50: 10000000 INFO @ Mon, 03 Jun 2019 13:50:53: 10000000 INFO @ Mon, 03 Jun 2019 13:50:56: 12000000 INFO @ Mon, 03 Jun 2019 13:51:00: 11000000 INFO @ Mon, 03 Jun 2019 13:51:04: 11000000 INFO @ Mon, 03 Jun 2019 13:51:04: 13000000 INFO @ Mon, 03 Jun 2019 13:51:10: 12000000 INFO @ Mon, 03 Jun 2019 13:51:13: 14000000 INFO @ Mon, 03 Jun 2019 13:51:13: 12000000 INFO @ Mon, 03 Jun 2019 13:51:19: 13000000 INFO @ Mon, 03 Jun 2019 13:51:21: 15000000 INFO @ Mon, 03 Jun 2019 13:51:23: 13000000 INFO @ Mon, 03 Jun 2019 13:51:28: 14000000 INFO @ Mon, 03 Jun 2019 13:51:30: 16000000 INFO @ Mon, 03 Jun 2019 13:51:34: 14000000 INFO @ Mon, 03 Jun 2019 13:51:38: 15000000 INFO @ Mon, 03 Jun 2019 13:51:39: 17000000 INFO @ Mon, 03 Jun 2019 13:51:44: 15000000 INFO @ Mon, 03 Jun 2019 13:51:47: 16000000 INFO @ Mon, 03 Jun 2019 13:51:47: 18000000 INFO @ Mon, 03 Jun 2019 13:51:53: 16000000 INFO @ Mon, 03 Jun 2019 13:51:56: 19000000 INFO @ Mon, 03 Jun 2019 13:51:56: 17000000 INFO @ Mon, 03 Jun 2019 13:52:03: 17000000 INFO @ Mon, 03 Jun 2019 13:52:04: 20000000 INFO @ Mon, 03 Jun 2019 13:52:06: 18000000 INFO @ Mon, 03 Jun 2019 13:52:13: 21000000 INFO @ Mon, 03 Jun 2019 13:52:13: 18000000 INFO @ Mon, 03 Jun 2019 13:52:15: 19000000 INFO @ Mon, 03 Jun 2019 13:52:21: 22000000 INFO @ Mon, 03 Jun 2019 13:52:22: 19000000 INFO @ Mon, 03 Jun 2019 13:52:25: 20000000 INFO @ Mon, 03 Jun 2019 13:52:29: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:52:29: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:52:29: #1 total tags in treatment: 22749672 INFO @ Mon, 03 Jun 2019 13:52:29: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:52:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:52:29: #1 tags after filtering in treatment: 22749672 INFO @ Mon, 03 Jun 2019 13:52:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:52:29: #1 finished! INFO @ Mon, 03 Jun 2019 13:52:29: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:52:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:52:31: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 13:52:31: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:52:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:52:32: 20000000 INFO @ Mon, 03 Jun 2019 13:52:34: 21000000 INFO @ Mon, 03 Jun 2019 13:52:41: 21000000 INFO @ Mon, 03 Jun 2019 13:52:43: 22000000 INFO @ Mon, 03 Jun 2019 13:52:51: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:52:51: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:52:51: #1 total tags in treatment: 22749672 INFO @ Mon, 03 Jun 2019 13:52:51: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:52:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:52:51: 22000000 INFO @ Mon, 03 Jun 2019 13:52:51: #1 tags after filtering in treatment: 22749672 INFO @ Mon, 03 Jun 2019 13:52:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:52:51: #1 finished! INFO @ Mon, 03 Jun 2019 13:52:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:52:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:52:53: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 13:52:53: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:52:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:52:58: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:52:58: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:52:58: #1 total tags in treatment: 22749672 INFO @ Mon, 03 Jun 2019 13:52:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:52:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:52:58: #1 tags after filtering in treatment: 22749672 INFO @ Mon, 03 Jun 2019 13:52:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:52:58: #1 finished! INFO @ Mon, 03 Jun 2019 13:52:58: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:52:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:53:00: #2 number of paired peaks: 1 WARNING @ Mon, 03 Jun 2019 13:53:00: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:53:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335505/SRX335505.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。