Job ID = 1295329 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,570,048 reads read : 22,570,048 reads written : 22,570,048 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:52 22570048 reads; of these: 22570048 (100.00%) were unpaired; of these: 4589344 (20.33%) aligned 0 times 15643536 (69.31%) aligned exactly 1 time 2337168 (10.36%) aligned >1 times 79.67% overall alignment rate Time searching: 00:05:52 Overall time: 00:05:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3637277 / 17980704 = 0.2023 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:31:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:31:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:31:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:31:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:31:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:31:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:31:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:31:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:31:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:31:57: 1000000 INFO @ Mon, 03 Jun 2019 13:31:59: 1000000 INFO @ Mon, 03 Jun 2019 13:31:59: 1000000 INFO @ Mon, 03 Jun 2019 13:32:05: 2000000 INFO @ Mon, 03 Jun 2019 13:32:08: 2000000 INFO @ Mon, 03 Jun 2019 13:32:08: 2000000 INFO @ Mon, 03 Jun 2019 13:32:13: 3000000 INFO @ Mon, 03 Jun 2019 13:32:18: 3000000 INFO @ Mon, 03 Jun 2019 13:32:18: 3000000 INFO @ Mon, 03 Jun 2019 13:32:21: 4000000 INFO @ Mon, 03 Jun 2019 13:32:27: 4000000 INFO @ Mon, 03 Jun 2019 13:32:27: 4000000 INFO @ Mon, 03 Jun 2019 13:32:29: 5000000 INFO @ Mon, 03 Jun 2019 13:32:36: 5000000 INFO @ Mon, 03 Jun 2019 13:32:36: 6000000 INFO @ Mon, 03 Jun 2019 13:32:36: 5000000 INFO @ Mon, 03 Jun 2019 13:32:44: 7000000 INFO @ Mon, 03 Jun 2019 13:32:45: 6000000 INFO @ Mon, 03 Jun 2019 13:32:46: 6000000 INFO @ Mon, 03 Jun 2019 13:32:52: 8000000 INFO @ Mon, 03 Jun 2019 13:32:54: 7000000 INFO @ Mon, 03 Jun 2019 13:32:55: 7000000 INFO @ Mon, 03 Jun 2019 13:33:00: 9000000 INFO @ Mon, 03 Jun 2019 13:33:03: 8000000 INFO @ Mon, 03 Jun 2019 13:33:04: 8000000 INFO @ Mon, 03 Jun 2019 13:33:07: 10000000 INFO @ Mon, 03 Jun 2019 13:33:12: 9000000 INFO @ Mon, 03 Jun 2019 13:33:13: 9000000 INFO @ Mon, 03 Jun 2019 13:33:15: 11000000 INFO @ Mon, 03 Jun 2019 13:33:21: 10000000 INFO @ Mon, 03 Jun 2019 13:33:23: 10000000 INFO @ Mon, 03 Jun 2019 13:33:23: 12000000 INFO @ Mon, 03 Jun 2019 13:33:30: 11000000 INFO @ Mon, 03 Jun 2019 13:33:31: 13000000 INFO @ Mon, 03 Jun 2019 13:33:32: 11000000 INFO @ Mon, 03 Jun 2019 13:33:39: 12000000 INFO @ Mon, 03 Jun 2019 13:33:39: 14000000 INFO @ Mon, 03 Jun 2019 13:33:41: 12000000 INFO @ Mon, 03 Jun 2019 13:33:42: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:33:42: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:33:42: #1 total tags in treatment: 14343427 INFO @ Mon, 03 Jun 2019 13:33:42: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:33:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:33:42: #1 tags after filtering in treatment: 14343427 INFO @ Mon, 03 Jun 2019 13:33:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:33:42: #1 finished! INFO @ Mon, 03 Jun 2019 13:33:42: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:33:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:33:43: #2 number of paired peaks: 90 WARNING @ Mon, 03 Jun 2019 13:33:43: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:33:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:33:48: 13000000 INFO @ Mon, 03 Jun 2019 13:33:50: 13000000 INFO @ Mon, 03 Jun 2019 13:33:56: 14000000 INFO @ Mon, 03 Jun 2019 13:33:59: 14000000 INFO @ Mon, 03 Jun 2019 13:33:59: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:33:59: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:33:59: #1 total tags in treatment: 14343427 INFO @ Mon, 03 Jun 2019 13:33:59: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:33:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:34:00: #1 tags after filtering in treatment: 14343427 INFO @ Mon, 03 Jun 2019 13:34:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:34:00: #1 finished! INFO @ Mon, 03 Jun 2019 13:34:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:34:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:34:01: #2 number of paired peaks: 90 WARNING @ Mon, 03 Jun 2019 13:34:01: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:34:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:34:02: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:34:02: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:34:02: #1 total tags in treatment: 14343427 INFO @ Mon, 03 Jun 2019 13:34:02: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:34:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:34:02: #1 tags after filtering in treatment: 14343427 INFO @ Mon, 03 Jun 2019 13:34:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:34:02: #1 finished! INFO @ Mon, 03 Jun 2019 13:34:02: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:34:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:34:04: #2 number of paired peaks: 90 WARNING @ Mon, 03 Jun 2019 13:34:04: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:34:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335493/SRX335493.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。