Job ID = 1295311 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,935,093 reads read : 47,935,093 reads written : 47,935,093 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:18 47935093 reads; of these: 47935093 (100.00%) were unpaired; of these: 4046889 (8.44%) aligned 0 times 36457738 (76.06%) aligned exactly 1 time 7430466 (15.50%) aligned >1 times 91.56% overall alignment rate Time searching: 00:13:18 Overall time: 00:13:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 16607238 / 43888204 = 0.3784 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:51:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:51:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:51:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:51:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:51:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:51:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:51:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:51:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:51:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:51:26: 1000000 INFO @ Mon, 03 Jun 2019 13:51:26: 1000000 INFO @ Mon, 03 Jun 2019 13:51:27: 1000000 INFO @ Mon, 03 Jun 2019 13:51:36: 2000000 INFO @ Mon, 03 Jun 2019 13:51:36: 2000000 INFO @ Mon, 03 Jun 2019 13:51:38: 2000000 INFO @ Mon, 03 Jun 2019 13:51:46: 3000000 INFO @ Mon, 03 Jun 2019 13:51:46: 3000000 INFO @ Mon, 03 Jun 2019 13:51:50: 3000000 INFO @ Mon, 03 Jun 2019 13:51:56: 4000000 INFO @ Mon, 03 Jun 2019 13:51:56: 4000000 INFO @ Mon, 03 Jun 2019 13:52:02: 4000000 INFO @ Mon, 03 Jun 2019 13:52:06: 5000000 INFO @ Mon, 03 Jun 2019 13:52:07: 5000000 INFO @ Mon, 03 Jun 2019 13:52:13: 5000000 INFO @ Mon, 03 Jun 2019 13:52:17: 6000000 INFO @ Mon, 03 Jun 2019 13:52:17: 6000000 INFO @ Mon, 03 Jun 2019 13:52:25: 6000000 INFO @ Mon, 03 Jun 2019 13:52:27: 7000000 INFO @ Mon, 03 Jun 2019 13:52:27: 7000000 INFO @ Mon, 03 Jun 2019 13:52:37: 7000000 INFO @ Mon, 03 Jun 2019 13:52:37: 8000000 INFO @ Mon, 03 Jun 2019 13:52:38: 8000000 INFO @ Mon, 03 Jun 2019 13:52:47: 9000000 INFO @ Mon, 03 Jun 2019 13:52:48: 9000000 INFO @ Mon, 03 Jun 2019 13:52:49: 8000000 INFO @ Mon, 03 Jun 2019 13:52:58: 10000000 INFO @ Mon, 03 Jun 2019 13:52:58: 10000000 INFO @ Mon, 03 Jun 2019 13:53:00: 9000000 INFO @ Mon, 03 Jun 2019 13:53:07: 11000000 INFO @ Mon, 03 Jun 2019 13:53:08: 11000000 INFO @ Mon, 03 Jun 2019 13:53:12: 10000000 INFO @ Mon, 03 Jun 2019 13:53:17: 12000000 INFO @ Mon, 03 Jun 2019 13:53:18: 12000000 INFO @ Mon, 03 Jun 2019 13:53:24: 11000000 INFO @ Mon, 03 Jun 2019 13:53:27: 13000000 INFO @ Mon, 03 Jun 2019 13:53:28: 13000000 INFO @ Mon, 03 Jun 2019 13:53:35: 12000000 INFO @ Mon, 03 Jun 2019 13:53:37: 14000000 INFO @ Mon, 03 Jun 2019 13:53:38: 14000000 INFO @ Mon, 03 Jun 2019 13:53:47: 13000000 INFO @ Mon, 03 Jun 2019 13:53:47: 15000000 INFO @ Mon, 03 Jun 2019 13:53:48: 15000000 INFO @ Mon, 03 Jun 2019 13:53:58: 16000000 INFO @ Mon, 03 Jun 2019 13:53:58: 14000000 INFO @ Mon, 03 Jun 2019 13:53:58: 16000000 INFO @ Mon, 03 Jun 2019 13:54:07: 17000000 INFO @ Mon, 03 Jun 2019 13:54:08: 17000000 INFO @ Mon, 03 Jun 2019 13:54:10: 15000000 INFO @ Mon, 03 Jun 2019 13:54:17: 18000000 INFO @ Mon, 03 Jun 2019 13:54:18: 18000000 INFO @ Mon, 03 Jun 2019 13:54:21: 16000000 INFO @ Mon, 03 Jun 2019 13:54:27: 19000000 INFO @ Mon, 03 Jun 2019 13:54:28: 19000000 INFO @ Mon, 03 Jun 2019 13:54:33: 17000000 INFO @ Mon, 03 Jun 2019 13:54:37: 20000000 INFO @ Mon, 03 Jun 2019 13:54:38: 20000000 INFO @ Mon, 03 Jun 2019 13:54:45: 18000000 INFO @ Mon, 03 Jun 2019 13:54:46: 21000000 INFO @ Mon, 03 Jun 2019 13:54:48: 21000000 INFO @ Mon, 03 Jun 2019 13:54:56: 22000000 INFO @ Mon, 03 Jun 2019 13:54:56: 19000000 INFO @ Mon, 03 Jun 2019 13:54:58: 22000000 INFO @ Mon, 03 Jun 2019 13:55:06: 23000000 INFO @ Mon, 03 Jun 2019 13:55:08: 23000000 INFO @ Mon, 03 Jun 2019 13:55:08: 20000000 INFO @ Mon, 03 Jun 2019 13:55:16: 24000000 INFO @ Mon, 03 Jun 2019 13:55:18: 24000000 INFO @ Mon, 03 Jun 2019 13:55:19: 21000000 INFO @ Mon, 03 Jun 2019 13:55:26: 25000000 INFO @ Mon, 03 Jun 2019 13:55:28: 25000000 INFO @ Mon, 03 Jun 2019 13:55:31: 22000000 INFO @ Mon, 03 Jun 2019 13:55:36: 26000000 INFO @ Mon, 03 Jun 2019 13:55:38: 26000000 INFO @ Mon, 03 Jun 2019 13:55:43: 23000000 INFO @ Mon, 03 Jun 2019 13:55:46: 27000000 INFO @ Mon, 03 Jun 2019 13:55:48: 27000000 INFO @ Mon, 03 Jun 2019 13:55:48: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:55:48: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:55:48: #1 total tags in treatment: 27280966 INFO @ Mon, 03 Jun 2019 13:55:48: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:55:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:55:49: #1 tags after filtering in treatment: 27280966 INFO @ Mon, 03 Jun 2019 13:55:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:55:49: #1 finished! INFO @ Mon, 03 Jun 2019 13:55:49: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:55:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:55:51: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:55:51: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:55:51: #1 total tags in treatment: 27280966 INFO @ Mon, 03 Jun 2019 13:55:51: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:55:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:55:51: #1 tags after filtering in treatment: 27280966 INFO @ Mon, 03 Jun 2019 13:55:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:55:51: #1 finished! INFO @ Mon, 03 Jun 2019 13:55:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:55:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:55:51: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 13:55:51: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:55:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:55:53: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 13:55:53: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:55:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:55:54: 24000000 INFO @ Mon, 03 Jun 2019 13:56:05: 25000000 INFO @ Mon, 03 Jun 2019 13:56:15: 26000000 INFO @ Mon, 03 Jun 2019 13:56:26: 27000000 INFO @ Mon, 03 Jun 2019 13:56:29: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:56:29: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:56:29: #1 total tags in treatment: 27280966 INFO @ Mon, 03 Jun 2019 13:56:29: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:56:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:56:29: #1 tags after filtering in treatment: 27280966 INFO @ Mon, 03 Jun 2019 13:56:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:56:29: #1 finished! INFO @ Mon, 03 Jun 2019 13:56:29: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:56:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:56:31: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 13:56:31: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:56:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335486/SRX335486.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。