Job ID = 1295258 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,235,844 reads read : 11,235,844 reads written : 11,235,844 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:54 11235844 reads; of these: 11235844 (100.00%) were unpaired; of these: 639418 (5.69%) aligned 0 times 9018421 (80.26%) aligned exactly 1 time 1578005 (14.04%) aligned >1 times 94.31% overall alignment rate Time searching: 00:03:54 Overall time: 00:03:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 715021 / 10596426 = 0.0675 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:09:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:09:57: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:09:57: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:09:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:09:57: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:09:57: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:09:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:09:57: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:09:57: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:10:06: 1000000 INFO @ Mon, 03 Jun 2019 13:10:07: 1000000 INFO @ Mon, 03 Jun 2019 13:10:07: 1000000 INFO @ Mon, 03 Jun 2019 13:10:15: 2000000 INFO @ Mon, 03 Jun 2019 13:10:16: 2000000 INFO @ Mon, 03 Jun 2019 13:10:16: 2000000 INFO @ Mon, 03 Jun 2019 13:10:25: 3000000 INFO @ Mon, 03 Jun 2019 13:10:25: 3000000 INFO @ Mon, 03 Jun 2019 13:10:26: 3000000 INFO @ Mon, 03 Jun 2019 13:10:34: 4000000 INFO @ Mon, 03 Jun 2019 13:10:34: 4000000 INFO @ Mon, 03 Jun 2019 13:10:35: 4000000 INFO @ Mon, 03 Jun 2019 13:10:42: 5000000 INFO @ Mon, 03 Jun 2019 13:10:43: 5000000 INFO @ Mon, 03 Jun 2019 13:10:44: 5000000 INFO @ Mon, 03 Jun 2019 13:10:51: 6000000 INFO @ Mon, 03 Jun 2019 13:10:51: 6000000 INFO @ Mon, 03 Jun 2019 13:10:54: 6000000 INFO @ Mon, 03 Jun 2019 13:11:00: 7000000 INFO @ Mon, 03 Jun 2019 13:11:00: 7000000 INFO @ Mon, 03 Jun 2019 13:11:03: 7000000 INFO @ Mon, 03 Jun 2019 13:11:09: 8000000 INFO @ Mon, 03 Jun 2019 13:11:09: 8000000 INFO @ Mon, 03 Jun 2019 13:11:12: 8000000 INFO @ Mon, 03 Jun 2019 13:11:18: 9000000 INFO @ Mon, 03 Jun 2019 13:11:18: 9000000 INFO @ Mon, 03 Jun 2019 13:11:22: 9000000 INFO @ Mon, 03 Jun 2019 13:11:26: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:11:26: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:11:26: #1 total tags in treatment: 9881405 INFO @ Mon, 03 Jun 2019 13:11:26: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:11:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:11:26: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:11:26: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:11:26: #1 total tags in treatment: 9881405 INFO @ Mon, 03 Jun 2019 13:11:26: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:11:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:11:26: #1 tags after filtering in treatment: 9881405 INFO @ Mon, 03 Jun 2019 13:11:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:11:26: #1 finished! INFO @ Mon, 03 Jun 2019 13:11:26: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:11:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:11:27: #1 tags after filtering in treatment: 9881405 INFO @ Mon, 03 Jun 2019 13:11:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:11:27: #1 finished! INFO @ Mon, 03 Jun 2019 13:11:27: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:11:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:11:27: #2 number of paired peaks: 15 WARNING @ Mon, 03 Jun 2019 13:11:27: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:11:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:11:27: #2 number of paired peaks: 15 WARNING @ Mon, 03 Jun 2019 13:11:27: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:11:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:11:30: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:11:30: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:11:30: #1 total tags in treatment: 9881405 INFO @ Mon, 03 Jun 2019 13:11:30: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:11:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:11:30: #1 tags after filtering in treatment: 9881405 INFO @ Mon, 03 Jun 2019 13:11:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:11:30: #1 finished! INFO @ Mon, 03 Jun 2019 13:11:30: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:11:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:11:31: #2 number of paired peaks: 15 WARNING @ Mon, 03 Jun 2019 13:11:31: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:11:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331412/SRX331412.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。