Job ID = 1295253 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,907,599 reads read : 11,907,599 reads written : 11,907,599 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:34 11907599 reads; of these: 11907599 (100.00%) were unpaired; of these: 560634 (4.71%) aligned 0 times 9676318 (81.26%) aligned exactly 1 time 1670647 (14.03%) aligned >1 times 95.29% overall alignment rate Time searching: 00:03:34 Overall time: 00:03:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 806195 / 11346965 = 0.0710 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:09:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:09:16: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:09:16: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:09:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:09:17: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:09:17: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:09:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:09:17: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:09:17: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:09:24: 1000000 INFO @ Mon, 03 Jun 2019 13:09:26: 1000000 INFO @ Mon, 03 Jun 2019 13:09:26: 1000000 INFO @ Mon, 03 Jun 2019 13:09:33: 2000000 INFO @ Mon, 03 Jun 2019 13:09:34: 2000000 INFO @ Mon, 03 Jun 2019 13:09:34: 2000000 INFO @ Mon, 03 Jun 2019 13:09:42: 3000000 INFO @ Mon, 03 Jun 2019 13:09:43: 3000000 INFO @ Mon, 03 Jun 2019 13:09:43: 3000000 INFO @ Mon, 03 Jun 2019 13:09:51: 4000000 INFO @ Mon, 03 Jun 2019 13:09:52: 4000000 INFO @ Mon, 03 Jun 2019 13:09:53: 4000000 INFO @ Mon, 03 Jun 2019 13:09:58: 5000000 INFO @ Mon, 03 Jun 2019 13:10:01: 5000000 INFO @ Mon, 03 Jun 2019 13:10:02: 5000000 INFO @ Mon, 03 Jun 2019 13:10:05: 6000000 INFO @ Mon, 03 Jun 2019 13:10:10: 6000000 INFO @ Mon, 03 Jun 2019 13:10:10: 6000000 INFO @ Mon, 03 Jun 2019 13:10:12: 7000000 INFO @ Mon, 03 Jun 2019 13:10:19: 7000000 INFO @ Mon, 03 Jun 2019 13:10:19: 7000000 INFO @ Mon, 03 Jun 2019 13:10:19: 8000000 INFO @ Mon, 03 Jun 2019 13:10:26: 9000000 INFO @ Mon, 03 Jun 2019 13:10:28: 8000000 INFO @ Mon, 03 Jun 2019 13:10:28: 8000000 INFO @ Mon, 03 Jun 2019 13:10:33: 10000000 INFO @ Mon, 03 Jun 2019 13:10:37: 9000000 INFO @ Mon, 03 Jun 2019 13:10:37: 9000000 INFO @ Mon, 03 Jun 2019 13:10:37: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:10:37: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:10:37: #1 total tags in treatment: 10540770 INFO @ Mon, 03 Jun 2019 13:10:37: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:10:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:10:37: #1 tags after filtering in treatment: 10540770 INFO @ Mon, 03 Jun 2019 13:10:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:10:37: #1 finished! INFO @ Mon, 03 Jun 2019 13:10:37: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:10:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:10:38: #2 number of paired peaks: 17 WARNING @ Mon, 03 Jun 2019 13:10:38: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:10:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:10:45: 10000000 INFO @ Mon, 03 Jun 2019 13:10:45: 10000000 INFO @ Mon, 03 Jun 2019 13:10:49: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:10:49: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:10:49: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:10:49: #1 total tags in treatment: 10540770 INFO @ Mon, 03 Jun 2019 13:10:49: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:10:49: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:10:49: #1 total tags in treatment: 10540770 INFO @ Mon, 03 Jun 2019 13:10:49: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:10:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:10:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:10:50: #1 tags after filtering in treatment: 10540770 INFO @ Mon, 03 Jun 2019 13:10:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:10:50: #1 finished! INFO @ Mon, 03 Jun 2019 13:10:50: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:10:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:10:50: #1 tags after filtering in treatment: 10540770 INFO @ Mon, 03 Jun 2019 13:10:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:10:50: #1 finished! INFO @ Mon, 03 Jun 2019 13:10:50: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:10:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:10:51: #2 number of paired peaks: 17 WARNING @ Mon, 03 Jun 2019 13:10:51: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:10:51: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 13:10:51: #2 number of paired peaks: 17 WARNING @ Mon, 03 Jun 2019 13:10:51: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:10:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331411/SRX331411.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。