Job ID = 6527945 SRX = SRX331409 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:08:50 prefetch.2.10.7: 1) Downloading 'SRR947646'... 2020-06-29T14:08:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:12:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:12:35 prefetch.2.10.7: 1) 'SRR947646' was downloaded successfully Read 19200904 spots for SRR947646/SRR947646.sra Written 19200904 spots for SRR947646/SRR947646.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:13 19200904 reads; of these: 19200904 (100.00%) were unpaired; of these: 1155366 (6.02%) aligned 0 times 13622429 (70.95%) aligned exactly 1 time 4423109 (23.04%) aligned >1 times 93.98% overall alignment rate Time searching: 00:06:13 Overall time: 00:06:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1407286 / 18045538 = 0.0780 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:28:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:28:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:28:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:28:43: 1000000 INFO @ Mon, 29 Jun 2020 23:28:48: 2000000 INFO @ Mon, 29 Jun 2020 23:28:54: 3000000 INFO @ Mon, 29 Jun 2020 23:28:59: 4000000 INFO @ Mon, 29 Jun 2020 23:29:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:08: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:08: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:10: 6000000 INFO @ Mon, 29 Jun 2020 23:29:14: 1000000 INFO @ Mon, 29 Jun 2020 23:29:16: 7000000 INFO @ Mon, 29 Jun 2020 23:29:20: 2000000 INFO @ Mon, 29 Jun 2020 23:29:22: 8000000 INFO @ Mon, 29 Jun 2020 23:29:26: 3000000 INFO @ Mon, 29 Jun 2020 23:29:28: 9000000 INFO @ Mon, 29 Jun 2020 23:29:31: 4000000 INFO @ Mon, 29 Jun 2020 23:29:34: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:37: 5000000 INFO @ Mon, 29 Jun 2020 23:29:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:41: 11000000 INFO @ Mon, 29 Jun 2020 23:29:44: 6000000 INFO @ Mon, 29 Jun 2020 23:29:45: 1000000 INFO @ Mon, 29 Jun 2020 23:29:47: 12000000 INFO @ Mon, 29 Jun 2020 23:29:51: 7000000 INFO @ Mon, 29 Jun 2020 23:29:53: 2000000 INFO @ Mon, 29 Jun 2020 23:29:54: 13000000 INFO @ Mon, 29 Jun 2020 23:29:58: 8000000 INFO @ Mon, 29 Jun 2020 23:30:01: 3000000 INFO @ Mon, 29 Jun 2020 23:30:01: 14000000 INFO @ Mon, 29 Jun 2020 23:30:04: 9000000 INFO @ Mon, 29 Jun 2020 23:30:07: 15000000 INFO @ Mon, 29 Jun 2020 23:30:08: 4000000 INFO @ Mon, 29 Jun 2020 23:30:11: 10000000 INFO @ Mon, 29 Jun 2020 23:30:14: 16000000 INFO @ Mon, 29 Jun 2020 23:30:16: 5000000 INFO @ Mon, 29 Jun 2020 23:30:18: 11000000 INFO @ Mon, 29 Jun 2020 23:30:18: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:30:18: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:30:18: #1 total tags in treatment: 16638252 INFO @ Mon, 29 Jun 2020 23:30:18: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:30:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:30:19: #1 tags after filtering in treatment: 16638252 INFO @ Mon, 29 Jun 2020 23:30:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:30:19: #1 finished! INFO @ Mon, 29 Jun 2020 23:30:19: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:30:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:30:20: #2 number of paired peaks: 50 WARNING @ Mon, 29 Jun 2020 23:30:20: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:30:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:30:23: 6000000 INFO @ Mon, 29 Jun 2020 23:30:24: 12000000 INFO @ Mon, 29 Jun 2020 23:30:31: 13000000 INFO @ Mon, 29 Jun 2020 23:30:31: 7000000 INFO @ Mon, 29 Jun 2020 23:30:38: 14000000 INFO @ Mon, 29 Jun 2020 23:30:38: 8000000 INFO @ Mon, 29 Jun 2020 23:30:44: 15000000 INFO @ Mon, 29 Jun 2020 23:30:46: 9000000 INFO @ Mon, 29 Jun 2020 23:30:50: 16000000 INFO @ Mon, 29 Jun 2020 23:30:53: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:30:55: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:30:55: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:30:55: #1 total tags in treatment: 16638252 INFO @ Mon, 29 Jun 2020 23:30:55: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:30:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:30:55: #1 tags after filtering in treatment: 16638252 INFO @ Mon, 29 Jun 2020 23:30:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:30:55: #1 finished! INFO @ Mon, 29 Jun 2020 23:30:55: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:30:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:30:56: #2 number of paired peaks: 50 WARNING @ Mon, 29 Jun 2020 23:30:56: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:30:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:31:00: 11000000 INFO @ Mon, 29 Jun 2020 23:31:07: 12000000 INFO @ Mon, 29 Jun 2020 23:31:14: 13000000 INFO @ Mon, 29 Jun 2020 23:31:20: 14000000 INFO @ Mon, 29 Jun 2020 23:31:27: 15000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:31:34: 16000000 INFO @ Mon, 29 Jun 2020 23:31:38: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:31:38: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:31:38: #1 total tags in treatment: 16638252 INFO @ Mon, 29 Jun 2020 23:31:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:31:38: #1 tags after filtering in treatment: 16638252 INFO @ Mon, 29 Jun 2020 23:31:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:31:38: #1 finished! INFO @ Mon, 29 Jun 2020 23:31:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:31:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:31:39: #2 number of paired peaks: 50 WARNING @ Mon, 29 Jun 2020 23:31:39: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:31:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331409/SRX331409.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling