Job ID = 6527944 SRX = SRX331405 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:08:21 prefetch.2.10.7: 1) Downloading 'SRR947642'... 2020-06-29T14:08:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:12:32 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:12:32 prefetch.2.10.7: 1) 'SRR947642' was downloaded successfully Read 19200904 spots for SRR947642/SRR947642.sra Written 19200904 spots for SRR947642/SRR947642.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:11 19200904 reads; of these: 19200904 (100.00%) were unpaired; of these: 1155350 (6.02%) aligned 0 times 13622426 (70.95%) aligned exactly 1 time 4423128 (23.04%) aligned >1 times 93.98% overall alignment rate Time searching: 00:06:11 Overall time: 00:06:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1406708 / 18045554 = 0.0780 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:28:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:28:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:28:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:28:44: 1000000 INFO @ Mon, 29 Jun 2020 23:28:51: 2000000 INFO @ Mon, 29 Jun 2020 23:28:57: 3000000 INFO @ Mon, 29 Jun 2020 23:29:04: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:08: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:08: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:10: 5000000 INFO @ Mon, 29 Jun 2020 23:29:15: 1000000 INFO @ Mon, 29 Jun 2020 23:29:17: 6000000 INFO @ Mon, 29 Jun 2020 23:29:22: 2000000 INFO @ Mon, 29 Jun 2020 23:29:24: 7000000 INFO @ Mon, 29 Jun 2020 23:29:30: 3000000 INFO @ Mon, 29 Jun 2020 23:29:31: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:37: 4000000 INFO @ Mon, 29 Jun 2020 23:29:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:39: 9000000 INFO @ Mon, 29 Jun 2020 23:29:45: 1000000 INFO @ Mon, 29 Jun 2020 23:29:45: 5000000 INFO @ Mon, 29 Jun 2020 23:29:46: 10000000 INFO @ Mon, 29 Jun 2020 23:29:52: 2000000 INFO @ Mon, 29 Jun 2020 23:29:53: 6000000 INFO @ Mon, 29 Jun 2020 23:29:53: 11000000 INFO @ Mon, 29 Jun 2020 23:29:59: 3000000 INFO @ Mon, 29 Jun 2020 23:30:00: 7000000 INFO @ Mon, 29 Jun 2020 23:30:01: 12000000 INFO @ Mon, 29 Jun 2020 23:30:06: 4000000 INFO @ Mon, 29 Jun 2020 23:30:07: 8000000 INFO @ Mon, 29 Jun 2020 23:30:08: 13000000 INFO @ Mon, 29 Jun 2020 23:30:13: 5000000 INFO @ Mon, 29 Jun 2020 23:30:14: 9000000 INFO @ Mon, 29 Jun 2020 23:30:16: 14000000 INFO @ Mon, 29 Jun 2020 23:30:20: 6000000 INFO @ Mon, 29 Jun 2020 23:30:21: 10000000 INFO @ Mon, 29 Jun 2020 23:30:23: 15000000 INFO @ Mon, 29 Jun 2020 23:30:27: 7000000 INFO @ Mon, 29 Jun 2020 23:30:29: 11000000 INFO @ Mon, 29 Jun 2020 23:30:30: 16000000 INFO @ Mon, 29 Jun 2020 23:30:34: 8000000 INFO @ Mon, 29 Jun 2020 23:30:35: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:30:35: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:30:35: #1 total tags in treatment: 16638846 INFO @ Mon, 29 Jun 2020 23:30:35: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:30:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:30:35: #1 tags after filtering in treatment: 16638846 INFO @ Mon, 29 Jun 2020 23:30:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:30:35: #1 finished! INFO @ Mon, 29 Jun 2020 23:30:35: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:30:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:30:36: #2 number of paired peaks: 55 WARNING @ Mon, 29 Jun 2020 23:30:36: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:30:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:30:36: 12000000 INFO @ Mon, 29 Jun 2020 23:30:41: 9000000 INFO @ Mon, 29 Jun 2020 23:30:44: 13000000 INFO @ Mon, 29 Jun 2020 23:30:48: 10000000 INFO @ Mon, 29 Jun 2020 23:30:51: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:30:54: 11000000 INFO @ Mon, 29 Jun 2020 23:30:58: 15000000 INFO @ Mon, 29 Jun 2020 23:31:01: 12000000 INFO @ Mon, 29 Jun 2020 23:31:05: 16000000 INFO @ Mon, 29 Jun 2020 23:31:08: 13000000 INFO @ Mon, 29 Jun 2020 23:31:10: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:31:10: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:31:10: #1 total tags in treatment: 16638846 INFO @ Mon, 29 Jun 2020 23:31:10: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:31:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:31:10: #1 tags after filtering in treatment: 16638846 INFO @ Mon, 29 Jun 2020 23:31:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:31:10: #1 finished! INFO @ Mon, 29 Jun 2020 23:31:10: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:31:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:31:11: #2 number of paired peaks: 55 WARNING @ Mon, 29 Jun 2020 23:31:11: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:31:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:31:15: 14000000 INFO @ Mon, 29 Jun 2020 23:31:21: 15000000 INFO @ Mon, 29 Jun 2020 23:31:28: 16000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:31:32: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:31:32: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:31:32: #1 total tags in treatment: 16638846 INFO @ Mon, 29 Jun 2020 23:31:32: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:31:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:31:32: #1 tags after filtering in treatment: 16638846 INFO @ Mon, 29 Jun 2020 23:31:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:31:32: #1 finished! INFO @ Mon, 29 Jun 2020 23:31:32: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:31:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:31:33: #2 number of paired peaks: 55 WARNING @ Mon, 29 Jun 2020 23:31:33: Too few paired peaks (55) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:31:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331405/SRX331405.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling