Job ID = 6527942 SRX = SRX331402 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:44:59 prefetch.2.10.7: 1) Downloading 'SRR947639'... 2020-06-29T13:44:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:48:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:48:02 prefetch.2.10.7: 1) 'SRR947639' was downloaded successfully Read 18463214 spots for SRR947639/SRR947639.sra Written 18463214 spots for SRR947639/SRR947639.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:40 18463214 reads; of these: 18463214 (100.00%) were unpaired; of these: 1216763 (6.59%) aligned 0 times 13413058 (72.65%) aligned exactly 1 time 3833393 (20.76%) aligned >1 times 93.41% overall alignment rate Time searching: 00:05:40 Overall time: 00:05:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1194700 / 17246451 = 0.0693 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:03:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:03:14: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:03:14: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:03:20: 1000000 INFO @ Mon, 29 Jun 2020 23:03:25: 2000000 INFO @ Mon, 29 Jun 2020 23:03:31: 3000000 INFO @ Mon, 29 Jun 2020 23:03:36: 4000000 INFO @ Mon, 29 Jun 2020 23:03:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:03:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:03:44: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:03:44: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:03:47: 6000000 INFO @ Mon, 29 Jun 2020 23:03:51: 1000000 INFO @ Mon, 29 Jun 2020 23:03:53: 7000000 INFO @ Mon, 29 Jun 2020 23:03:57: 2000000 INFO @ Mon, 29 Jun 2020 23:03:59: 8000000 INFO @ Mon, 29 Jun 2020 23:04:03: 3000000 INFO @ Mon, 29 Jun 2020 23:04:05: 9000000 INFO @ Mon, 29 Jun 2020 23:04:09: 4000000 INFO @ Mon, 29 Jun 2020 23:04:11: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:04:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:04:14: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:04:14: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:04:15: 5000000 INFO @ Mon, 29 Jun 2020 23:04:17: 11000000 INFO @ Mon, 29 Jun 2020 23:04:21: 1000000 INFO @ Mon, 29 Jun 2020 23:04:21: 6000000 INFO @ Mon, 29 Jun 2020 23:04:23: 12000000 INFO @ Mon, 29 Jun 2020 23:04:27: 2000000 INFO @ Mon, 29 Jun 2020 23:04:27: 7000000 INFO @ Mon, 29 Jun 2020 23:04:30: 13000000 INFO @ Mon, 29 Jun 2020 23:04:33: 3000000 INFO @ Mon, 29 Jun 2020 23:04:33: 8000000 INFO @ Mon, 29 Jun 2020 23:04:36: 14000000 INFO @ Mon, 29 Jun 2020 23:04:39: 4000000 INFO @ Mon, 29 Jun 2020 23:04:39: 9000000 INFO @ Mon, 29 Jun 2020 23:04:42: 15000000 INFO @ Mon, 29 Jun 2020 23:04:45: 5000000 INFO @ Mon, 29 Jun 2020 23:04:45: 10000000 INFO @ Mon, 29 Jun 2020 23:04:48: 16000000 INFO @ Mon, 29 Jun 2020 23:04:49: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:04:49: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:04:49: #1 total tags in treatment: 16051751 INFO @ Mon, 29 Jun 2020 23:04:49: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:04:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:04:49: #1 tags after filtering in treatment: 16051751 INFO @ Mon, 29 Jun 2020 23:04:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:04:49: #1 finished! INFO @ Mon, 29 Jun 2020 23:04:49: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:04:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:04:50: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 23:04:50: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:04:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:04:51: 6000000 INFO @ Mon, 29 Jun 2020 23:04:52: 11000000 INFO @ Mon, 29 Jun 2020 23:04:57: 7000000 INFO @ Mon, 29 Jun 2020 23:04:58: 12000000 INFO @ Mon, 29 Jun 2020 23:05:03: 8000000 INFO @ Mon, 29 Jun 2020 23:05:04: 13000000 INFO @ Mon, 29 Jun 2020 23:05:09: 9000000 INFO @ Mon, 29 Jun 2020 23:05:10: 14000000 INFO @ Mon, 29 Jun 2020 23:05:15: 10000000 INFO @ Mon, 29 Jun 2020 23:05:16: 15000000 INFO @ Mon, 29 Jun 2020 23:05:22: 11000000 INFO @ Mon, 29 Jun 2020 23:05:23: 16000000 INFO @ Mon, 29 Jun 2020 23:05:23: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:05:23: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:05:23: #1 total tags in treatment: 16051751 INFO @ Mon, 29 Jun 2020 23:05:23: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:05:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:05:23: #1 tags after filtering in treatment: 16051751 INFO @ Mon, 29 Jun 2020 23:05:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:05:23: #1 finished! INFO @ Mon, 29 Jun 2020 23:05:23: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:05:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:05:24: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 23:05:24: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:05:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:05:28: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:05:33: 13000000 INFO @ Mon, 29 Jun 2020 23:05:39: 14000000 INFO @ Mon, 29 Jun 2020 23:05:45: 15000000 INFO @ Mon, 29 Jun 2020 23:05:50: 16000000 INFO @ Mon, 29 Jun 2020 23:05:51: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:05:51: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:05:51: #1 total tags in treatment: 16051751 INFO @ Mon, 29 Jun 2020 23:05:51: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:05:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:05:51: #1 tags after filtering in treatment: 16051751 INFO @ Mon, 29 Jun 2020 23:05:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:05:51: #1 finished! INFO @ Mon, 29 Jun 2020 23:05:51: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:05:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:05:52: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 23:05:52: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:05:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX331402/SRX331402.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。