Job ID = 1295229 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,988,235 reads read : 18,988,235 reads written : 18,988,235 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:39 18988235 reads; of these: 18988235 (100.00%) were unpaired; of these: 2948645 (15.53%) aligned 0 times 13066803 (68.82%) aligned exactly 1 time 2972787 (15.66%) aligned >1 times 84.47% overall alignment rate Time searching: 00:05:39 Overall time: 00:05:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6612767 / 16039590 = 0.4123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:05:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:05:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:05:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:05:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:05:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:05:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:05:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:05:11: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:05:11: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:05:20: 1000000 INFO @ Mon, 03 Jun 2019 13:05:20: 1000000 INFO @ Mon, 03 Jun 2019 13:05:21: 1000000 INFO @ Mon, 03 Jun 2019 13:05:28: 2000000 INFO @ Mon, 03 Jun 2019 13:05:29: 2000000 INFO @ Mon, 03 Jun 2019 13:05:31: 2000000 INFO @ Mon, 03 Jun 2019 13:05:36: 3000000 INFO @ Mon, 03 Jun 2019 13:05:37: 3000000 INFO @ Mon, 03 Jun 2019 13:05:42: 3000000 INFO @ Mon, 03 Jun 2019 13:05:43: 4000000 INFO @ Mon, 03 Jun 2019 13:05:45: 4000000 INFO @ Mon, 03 Jun 2019 13:05:50: 5000000 INFO @ Mon, 03 Jun 2019 13:05:52: 4000000 INFO @ Mon, 03 Jun 2019 13:05:54: 5000000 INFO @ Mon, 03 Jun 2019 13:05:58: 6000000 INFO @ Mon, 03 Jun 2019 13:06:01: 5000000 INFO @ Mon, 03 Jun 2019 13:06:03: 6000000 INFO @ Mon, 03 Jun 2019 13:06:06: 7000000 INFO @ Mon, 03 Jun 2019 13:06:11: 6000000 INFO @ Mon, 03 Jun 2019 13:06:12: 7000000 INFO @ Mon, 03 Jun 2019 13:06:13: 8000000 INFO @ Mon, 03 Jun 2019 13:06:20: 8000000 INFO @ Mon, 03 Jun 2019 13:06:21: 9000000 INFO @ Mon, 03 Jun 2019 13:06:22: 7000000 INFO @ Mon, 03 Jun 2019 13:06:24: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:06:24: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:06:24: #1 total tags in treatment: 9426823 INFO @ Mon, 03 Jun 2019 13:06:24: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:06:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:06:24: #1 tags after filtering in treatment: 9426823 INFO @ Mon, 03 Jun 2019 13:06:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:06:24: #1 finished! INFO @ Mon, 03 Jun 2019 13:06:24: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:06:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:06:25: #2 number of paired peaks: 2570 INFO @ Mon, 03 Jun 2019 13:06:25: start model_add_line... INFO @ Mon, 03 Jun 2019 13:06:25: start X-correlation... INFO @ Mon, 03 Jun 2019 13:06:25: end of X-cor INFO @ Mon, 03 Jun 2019 13:06:25: #2 finished! INFO @ Mon, 03 Jun 2019 13:06:25: #2 predicted fragment length is 245 bps INFO @ Mon, 03 Jun 2019 13:06:25: #2 alternative fragment length(s) may be 245 bps INFO @ Mon, 03 Jun 2019 13:06:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.20_model.r INFO @ Mon, 03 Jun 2019 13:06:25: #3 Call peaks... INFO @ Mon, 03 Jun 2019 13:06:25: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 13:06:29: 9000000 INFO @ Mon, 03 Jun 2019 13:06:32: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:06:32: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:06:32: #1 total tags in treatment: 9426823 INFO @ Mon, 03 Jun 2019 13:06:32: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:06:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:06:32: #1 tags after filtering in treatment: 9426823 INFO @ Mon, 03 Jun 2019 13:06:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:06:32: #1 finished! INFO @ Mon, 03 Jun 2019 13:06:32: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:06:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:06:32: 8000000 INFO @ Mon, 03 Jun 2019 13:06:33: #2 number of paired peaks: 2570 INFO @ Mon, 03 Jun 2019 13:06:33: start model_add_line... INFO @ Mon, 03 Jun 2019 13:06:33: start X-correlation... INFO @ Mon, 03 Jun 2019 13:06:33: end of X-cor INFO @ Mon, 03 Jun 2019 13:06:33: #2 finished! INFO @ Mon, 03 Jun 2019 13:06:33: #2 predicted fragment length is 245 bps INFO @ Mon, 03 Jun 2019 13:06:33: #2 alternative fragment length(s) may be 245 bps INFO @ Mon, 03 Jun 2019 13:06:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.10_model.r INFO @ Mon, 03 Jun 2019 13:06:33: #3 Call peaks... INFO @ Mon, 03 Jun 2019 13:06:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 13:06:42: 9000000 INFO @ Mon, 03 Jun 2019 13:06:46: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:06:46: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:06:46: #1 total tags in treatment: 9426823 INFO @ Mon, 03 Jun 2019 13:06:46: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:06:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:06:47: #1 tags after filtering in treatment: 9426823 INFO @ Mon, 03 Jun 2019 13:06:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:06:47: #1 finished! INFO @ Mon, 03 Jun 2019 13:06:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:06:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:06:48: #2 number of paired peaks: 2570 INFO @ Mon, 03 Jun 2019 13:06:48: start model_add_line... INFO @ Mon, 03 Jun 2019 13:06:48: start X-correlation... INFO @ Mon, 03 Jun 2019 13:06:48: end of X-cor INFO @ Mon, 03 Jun 2019 13:06:48: #2 finished! INFO @ Mon, 03 Jun 2019 13:06:48: #2 predicted fragment length is 245 bps INFO @ Mon, 03 Jun 2019 13:06:48: #2 alternative fragment length(s) may be 245 bps INFO @ Mon, 03 Jun 2019 13:06:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.05_model.r INFO @ Mon, 03 Jun 2019 13:06:48: #3 Call peaks... INFO @ Mon, 03 Jun 2019 13:06:48: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 13:06:57: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 13:07:05: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 13:07:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.20_peaks.xls INFO @ Mon, 03 Jun 2019 13:07:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 13:07:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.20_summits.bed INFO @ Mon, 03 Jun 2019 13:07:12: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (3338 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:07:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.10_peaks.xls INFO @ Mon, 03 Jun 2019 13:07:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 13:07:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.10_summits.bed INFO @ Mon, 03 Jun 2019 13:07:20: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (4546 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:07:21: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 13:07:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.05_peaks.xls INFO @ Mon, 03 Jun 2019 13:07:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 13:07:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX331399/SRX331399.05_summits.bed INFO @ Mon, 03 Jun 2019 13:07:36: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (5786 records, 4 fields): 10 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。