Job ID = 1295135 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-03T03:10:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T03:10:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T03:11:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T03:11:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T03:20:41 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 18,270,299 reads read : 36,540,598 reads written : 36,540,598 2019-06-03T03:30:41 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 18,452,331 reads read : 36,904,662 reads written : 36,904,662 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:03:04 36722630 reads; of these: 36722630 (100.00%) were paired; of these: 6617760 (18.02%) aligned concordantly 0 times 22871945 (62.28%) aligned concordantly exactly 1 time 7232925 (19.70%) aligned concordantly >1 times ---- 6617760 pairs aligned concordantly 0 times; of these: 387986 (5.86%) aligned discordantly 1 time ---- 6229774 pairs aligned 0 times concordantly or discordantly; of these: 12459548 mates make up the pairs; of these: 9582050 (76.91%) aligned 0 times 1926729 (15.46%) aligned exactly 1 time 950769 (7.63%) aligned >1 times 86.95% overall alignment rate Time searching: 01:03:04 Overall time: 01:03:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3467571 / 30324962 = 0.1143 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 14:12:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 14:12:10: #1 read tag files... INFO @ Mon, 03 Jun 2019 14:12:10: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 14:12:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 14:12:10: #1 read tag files... INFO @ Mon, 03 Jun 2019 14:12:10: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 14:12:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 14:12:10: #1 read tag files... INFO @ Mon, 03 Jun 2019 14:12:10: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 14:12:18: 1000000 INFO @ Mon, 03 Jun 2019 14:12:18: 1000000 INFO @ Mon, 03 Jun 2019 14:12:21: 1000000 INFO @ Mon, 03 Jun 2019 14:12:26: 2000000 INFO @ Mon, 03 Jun 2019 14:12:26: 2000000 INFO @ Mon, 03 Jun 2019 14:12:32: 2000000 INFO @ Mon, 03 Jun 2019 14:12:34: 3000000 INFO @ Mon, 03 Jun 2019 14:12:34: 3000000 INFO @ Mon, 03 Jun 2019 14:12:42: 4000000 INFO @ Mon, 03 Jun 2019 14:12:42: 4000000 INFO @ Mon, 03 Jun 2019 14:12:43: 3000000 INFO @ Mon, 03 Jun 2019 14:12:50: 5000000 INFO @ Mon, 03 Jun 2019 14:12:50: 5000000 INFO @ Mon, 03 Jun 2019 14:12:53: 4000000 INFO @ Mon, 03 Jun 2019 14:12:58: 6000000 INFO @ Mon, 03 Jun 2019 14:12:59: 6000000 INFO @ Mon, 03 Jun 2019 14:13:04: 5000000 INFO @ Mon, 03 Jun 2019 14:13:06: 7000000 INFO @ Mon, 03 Jun 2019 14:13:06: 7000000 INFO @ Mon, 03 Jun 2019 14:13:13: 8000000 INFO @ Mon, 03 Jun 2019 14:13:14: 8000000 INFO @ Mon, 03 Jun 2019 14:13:15: 6000000 INFO @ Mon, 03 Jun 2019 14:13:21: 9000000 INFO @ Mon, 03 Jun 2019 14:13:22: 9000000 INFO @ Mon, 03 Jun 2019 14:13:26: 7000000 INFO @ Mon, 03 Jun 2019 14:13:29: 10000000 INFO @ Mon, 03 Jun 2019 14:13:29: 10000000 INFO @ Mon, 03 Jun 2019 14:13:36: 11000000 INFO @ Mon, 03 Jun 2019 14:13:36: 8000000 INFO @ Mon, 03 Jun 2019 14:13:37: 11000000 INFO @ Mon, 03 Jun 2019 14:13:44: 12000000 INFO @ Mon, 03 Jun 2019 14:13:45: 12000000 INFO @ Mon, 03 Jun 2019 14:13:47: 9000000 INFO @ Mon, 03 Jun 2019 14:13:51: 13000000 INFO @ Mon, 03 Jun 2019 14:13:52: 13000000 INFO @ Mon, 03 Jun 2019 14:13:58: 10000000 INFO @ Mon, 03 Jun 2019 14:13:59: 14000000 INFO @ Mon, 03 Jun 2019 14:14:00: 14000000 INFO @ Mon, 03 Jun 2019 14:14:07: 15000000 INFO @ Mon, 03 Jun 2019 14:14:08: 15000000 INFO @ Mon, 03 Jun 2019 14:14:09: 11000000 INFO @ Mon, 03 Jun 2019 14:14:16: 16000000 INFO @ Mon, 03 Jun 2019 14:14:17: 16000000 INFO @ Mon, 03 Jun 2019 14:14:20: 12000000 INFO @ Mon, 03 Jun 2019 14:14:25: 17000000 INFO @ Mon, 03 Jun 2019 14:14:26: 17000000 INFO @ Mon, 03 Jun 2019 14:14:31: 13000000 INFO @ Mon, 03 Jun 2019 14:14:34: 18000000 INFO @ Mon, 03 Jun 2019 14:14:35: 18000000 INFO @ Mon, 03 Jun 2019 14:14:41: 14000000 INFO @ Mon, 03 Jun 2019 14:14:43: 19000000 INFO @ Mon, 03 Jun 2019 14:14:43: 19000000 INFO @ Mon, 03 Jun 2019 14:14:52: 15000000 INFO @ Mon, 03 Jun 2019 14:14:52: 20000000 INFO @ Mon, 03 Jun 2019 14:14:52: 20000000 INFO @ Mon, 03 Jun 2019 14:15:01: 21000000 INFO @ Mon, 03 Jun 2019 14:15:02: 21000000 INFO @ Mon, 03 Jun 2019 14:15:03: 16000000 INFO @ Mon, 03 Jun 2019 14:15:11: 22000000 INFO @ Mon, 03 Jun 2019 14:15:11: 22000000 INFO @ Mon, 03 Jun 2019 14:15:14: 17000000 INFO @ Mon, 03 Jun 2019 14:15:19: 23000000 INFO @ Mon, 03 Jun 2019 14:15:21: 23000000 INFO @ Mon, 03 Jun 2019 14:15:24: 18000000 INFO @ Mon, 03 Jun 2019 14:15:26: 24000000 INFO @ Mon, 03 Jun 2019 14:15:29: 24000000 INFO @ Mon, 03 Jun 2019 14:15:34: 25000000 INFO @ Mon, 03 Jun 2019 14:15:34: 19000000 INFO @ Mon, 03 Jun 2019 14:15:36: 25000000 INFO @ Mon, 03 Jun 2019 14:15:41: 26000000 INFO @ Mon, 03 Jun 2019 14:15:44: 26000000 INFO @ Mon, 03 Jun 2019 14:15:45: 20000000 INFO @ Mon, 03 Jun 2019 14:15:49: 27000000 INFO @ Mon, 03 Jun 2019 14:15:51: 27000000 INFO @ Mon, 03 Jun 2019 14:15:55: 21000000 INFO @ Mon, 03 Jun 2019 14:15:57: 28000000 INFO @ Mon, 03 Jun 2019 14:15:59: 28000000 INFO @ Mon, 03 Jun 2019 14:16:04: 29000000 INFO @ Mon, 03 Jun 2019 14:16:05: 22000000 INFO @ Mon, 03 Jun 2019 14:16:07: 29000000 INFO @ Mon, 03 Jun 2019 14:16:12: 30000000 INFO @ Mon, 03 Jun 2019 14:16:14: 30000000 INFO @ Mon, 03 Jun 2019 14:16:16: 23000000 INFO @ Mon, 03 Jun 2019 14:16:19: 31000000 INFO @ Mon, 03 Jun 2019 14:16:22: 31000000 INFO @ Mon, 03 Jun 2019 14:16:26: 24000000 INFO @ Mon, 03 Jun 2019 14:16:28: 32000000 INFO @ Mon, 03 Jun 2019 14:16:30: 32000000 INFO @ Mon, 03 Jun 2019 14:16:36: 33000000 INFO @ Mon, 03 Jun 2019 14:16:37: 33000000 INFO @ Mon, 03 Jun 2019 14:16:37: 25000000 INFO @ Mon, 03 Jun 2019 14:16:43: 34000000 INFO @ Mon, 03 Jun 2019 14:16:45: 34000000 INFO @ Mon, 03 Jun 2019 14:16:48: 26000000 INFO @ Mon, 03 Jun 2019 14:16:51: 35000000 INFO @ Mon, 03 Jun 2019 14:16:52: 35000000 INFO @ Mon, 03 Jun 2019 14:16:58: 27000000 INFO @ Mon, 03 Jun 2019 14:16:58: 36000000 INFO @ Mon, 03 Jun 2019 14:17:00: 36000000 INFO @ Mon, 03 Jun 2019 14:17:06: 37000000 INFO @ Mon, 03 Jun 2019 14:17:07: 37000000 INFO @ Mon, 03 Jun 2019 14:17:08: 28000000 INFO @ Mon, 03 Jun 2019 14:17:14: 38000000 INFO @ Mon, 03 Jun 2019 14:17:15: 38000000 INFO @ Mon, 03 Jun 2019 14:17:19: 29000000 INFO @ Mon, 03 Jun 2019 14:17:21: 39000000 INFO @ Mon, 03 Jun 2019 14:17:23: 39000000 INFO @ Mon, 03 Jun 2019 14:17:29: 40000000 INFO @ Mon, 03 Jun 2019 14:17:29: 30000000 INFO @ Mon, 03 Jun 2019 14:17:30: 40000000 INFO @ Mon, 03 Jun 2019 14:17:36: 41000000 INFO @ Mon, 03 Jun 2019 14:17:38: 41000000 INFO @ Mon, 03 Jun 2019 14:17:39: 31000000 INFO @ Mon, 03 Jun 2019 14:17:44: 42000000 INFO @ Mon, 03 Jun 2019 14:17:45: 42000000 INFO @ Mon, 03 Jun 2019 14:17:50: 32000000 INFO @ Mon, 03 Jun 2019 14:17:51: 43000000 INFO @ Mon, 03 Jun 2019 14:17:52: 43000000 INFO @ Mon, 03 Jun 2019 14:17:58: 44000000 INFO @ Mon, 03 Jun 2019 14:18:00: 44000000 INFO @ Mon, 03 Jun 2019 14:18:00: 33000000 INFO @ Mon, 03 Jun 2019 14:18:06: 45000000 INFO @ Mon, 03 Jun 2019 14:18:07: 45000000 INFO @ Mon, 03 Jun 2019 14:18:10: 34000000 INFO @ Mon, 03 Jun 2019 14:18:13: 46000000 INFO @ Mon, 03 Jun 2019 14:18:15: 46000000 INFO @ Mon, 03 Jun 2019 14:18:21: 35000000 INFO @ Mon, 03 Jun 2019 14:18:21: 47000000 INFO @ Mon, 03 Jun 2019 14:18:22: 47000000 INFO @ Mon, 03 Jun 2019 14:18:28: 48000000 INFO @ Mon, 03 Jun 2019 14:18:29: 48000000 INFO @ Mon, 03 Jun 2019 14:18:31: 36000000 INFO @ Mon, 03 Jun 2019 14:18:36: 49000000 INFO @ Mon, 03 Jun 2019 14:18:37: 49000000 INFO @ Mon, 03 Jun 2019 14:18:41: 37000000 INFO @ Mon, 03 Jun 2019 14:18:43: 50000000 INFO @ Mon, 03 Jun 2019 14:18:44: 50000000 INFO @ Mon, 03 Jun 2019 14:18:51: 51000000 INFO @ Mon, 03 Jun 2019 14:18:51: 38000000 INFO @ Mon, 03 Jun 2019 14:18:52: 51000000 INFO @ Mon, 03 Jun 2019 14:18:58: 52000000 INFO @ Mon, 03 Jun 2019 14:18:59: 52000000 INFO @ Mon, 03 Jun 2019 14:19:02: 39000000 INFO @ Mon, 03 Jun 2019 14:19:05: 53000000 INFO @ Mon, 03 Jun 2019 14:19:07: 53000000 INFO @ Mon, 03 Jun 2019 14:19:12: 40000000 INFO @ Mon, 03 Jun 2019 14:19:13: 54000000 INFO @ Mon, 03 Jun 2019 14:19:14: 54000000 INFO @ Mon, 03 Jun 2019 14:19:20: 55000000 INFO @ Mon, 03 Jun 2019 14:19:22: 55000000 INFO @ Mon, 03 Jun 2019 14:19:22: 41000000 INFO @ Mon, 03 Jun 2019 14:19:28: 56000000 INFO @ Mon, 03 Jun 2019 14:19:29: 56000000 INFO @ Mon, 03 Jun 2019 14:19:32: 42000000 INFO @ Mon, 03 Jun 2019 14:19:35: #1 tag size is determined as 49 bps INFO @ Mon, 03 Jun 2019 14:19:35: #1 tag size = 49 INFO @ Mon, 03 Jun 2019 14:19:35: #1 total tags in treatment: 26643691 INFO @ Mon, 03 Jun 2019 14:19:35: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:19:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:19:36: #1 tags after filtering in treatment: 18763286 INFO @ Mon, 03 Jun 2019 14:19:36: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 14:19:36: #1 finished! INFO @ Mon, 03 Jun 2019 14:19:36: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:19:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:19:36: #1 tag size is determined as 49 bps INFO @ Mon, 03 Jun 2019 14:19:36: #1 tag size = 49 INFO @ Mon, 03 Jun 2019 14:19:36: #1 total tags in treatment: 26643691 INFO @ Mon, 03 Jun 2019 14:19:36: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:19:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:19:37: #1 tags after filtering in treatment: 18763286 INFO @ Mon, 03 Jun 2019 14:19:37: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 14:19:37: #1 finished! INFO @ Mon, 03 Jun 2019 14:19:37: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:19:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:19:37: #2 number of paired peaks: 383 WARNING @ Mon, 03 Jun 2019 14:19:37: Fewer paired peaks (383) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 383 pairs to build model! INFO @ Mon, 03 Jun 2019 14:19:37: start model_add_line... INFO @ Mon, 03 Jun 2019 14:19:37: start X-correlation... INFO @ Mon, 03 Jun 2019 14:19:37: end of X-cor INFO @ Mon, 03 Jun 2019 14:19:37: #2 finished! INFO @ Mon, 03 Jun 2019 14:19:37: #2 predicted fragment length is 109 bps INFO @ Mon, 03 Jun 2019 14:19:37: #2 alternative fragment length(s) may be 1,109,556 bps INFO @ Mon, 03 Jun 2019 14:19:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.20_model.r INFO @ Mon, 03 Jun 2019 14:19:37: #3 Call peaks... INFO @ Mon, 03 Jun 2019 14:19:37: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 14:19:38: #2 number of paired peaks: 383 WARNING @ Mon, 03 Jun 2019 14:19:38: Fewer paired peaks (383) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 383 pairs to build model! INFO @ Mon, 03 Jun 2019 14:19:38: start model_add_line... INFO @ Mon, 03 Jun 2019 14:19:39: start X-correlation... INFO @ Mon, 03 Jun 2019 14:19:39: end of X-cor INFO @ Mon, 03 Jun 2019 14:19:39: #2 finished! INFO @ Mon, 03 Jun 2019 14:19:39: #2 predicted fragment length is 109 bps INFO @ Mon, 03 Jun 2019 14:19:39: #2 alternative fragment length(s) may be 1,109,556 bps INFO @ Mon, 03 Jun 2019 14:19:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.10_model.r INFO @ Mon, 03 Jun 2019 14:19:39: #3 Call peaks... INFO @ Mon, 03 Jun 2019 14:19:39: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 14:19:41: 43000000 INFO @ Mon, 03 Jun 2019 14:19:51: 44000000 INFO @ Mon, 03 Jun 2019 14:20:00: 45000000 INFO @ Mon, 03 Jun 2019 14:20:10: 46000000 INFO @ Mon, 03 Jun 2019 14:20:19: 47000000 INFO @ Mon, 03 Jun 2019 14:20:28: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 14:20:29: 48000000 INFO @ Mon, 03 Jun 2019 14:20:29: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 14:20:38: 49000000 INFO @ Mon, 03 Jun 2019 14:20:48: 50000000 INFO @ Mon, 03 Jun 2019 14:20:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.20_peaks.xls INFO @ Mon, 03 Jun 2019 14:20:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 14:20:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.20_summits.bed INFO @ Mon, 03 Jun 2019 14:20:52: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1756 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 14:20:54: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.10_peaks.xls INFO @ Mon, 03 Jun 2019 14:20:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 14:20:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.10_summits.bed INFO @ Mon, 03 Jun 2019 14:20:54: Done! pass1 - making usageList (14 chroms): 3 millis pass2 - checking and writing primary data (6435 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 14:20:57: 51000000 INFO @ Mon, 03 Jun 2019 14:21:06: 52000000 INFO @ Mon, 03 Jun 2019 14:21:16: 53000000 INFO @ Mon, 03 Jun 2019 14:21:25: 54000000 INFO @ Mon, 03 Jun 2019 14:21:35: 55000000 INFO @ Mon, 03 Jun 2019 14:21:44: 56000000 INFO @ Mon, 03 Jun 2019 14:21:54: #1 tag size is determined as 49 bps INFO @ Mon, 03 Jun 2019 14:21:54: #1 tag size = 49 INFO @ Mon, 03 Jun 2019 14:21:54: #1 total tags in treatment: 26643691 INFO @ Mon, 03 Jun 2019 14:21:54: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:21:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:21:54: #1 tags after filtering in treatment: 18763286 INFO @ Mon, 03 Jun 2019 14:21:54: #1 Redundant rate of treatment: 0.30 INFO @ Mon, 03 Jun 2019 14:21:54: #1 finished! INFO @ Mon, 03 Jun 2019 14:21:54: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:21:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:21:56: #2 number of paired peaks: 383 WARNING @ Mon, 03 Jun 2019 14:21:56: Fewer paired peaks (383) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 383 pairs to build model! INFO @ Mon, 03 Jun 2019 14:21:56: start model_add_line... INFO @ Mon, 03 Jun 2019 14:21:56: start X-correlation... INFO @ Mon, 03 Jun 2019 14:21:56: end of X-cor INFO @ Mon, 03 Jun 2019 14:21:56: #2 finished! INFO @ Mon, 03 Jun 2019 14:21:56: #2 predicted fragment length is 109 bps INFO @ Mon, 03 Jun 2019 14:21:56: #2 alternative fragment length(s) may be 1,109,556 bps INFO @ Mon, 03 Jun 2019 14:21:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.05_model.r INFO @ Mon, 03 Jun 2019 14:21:56: #3 Call peaks... INFO @ Mon, 03 Jun 2019 14:21:56: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 14:22:47: #3 Call peaks for each chromosome... BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 03 Jun 2019 14:23:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.05_peaks.xls INFO @ Mon, 03 Jun 2019 14:23:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 14:23:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX326993/SRX326993.05_summits.bed INFO @ Mon, 03 Jun 2019 14:23:12: Done! pass1 - making usageList (15 chroms): 5 millis pass2 - checking and writing primary data (11416 records, 4 fields): 16 millis CompletedMACS2peakCalling BigWig に変換しました。