Job ID = 6527915 SRX = SRX318791 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:53:45 prefetch.2.10.7: 1) Downloading 'SRR927107'... 2020-06-29T13:53:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:57:52 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:57:52 prefetch.2.10.7: 1) 'SRR927107' was downloaded successfully Read 21014950 spots for SRR927107/SRR927107.sra Written 21014950 spots for SRR927107/SRR927107.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:03 21014950 reads; of these: 21014950 (100.00%) were unpaired; of these: 1185193 (5.64%) aligned 0 times 14968728 (71.23%) aligned exactly 1 time 4861029 (23.13%) aligned >1 times 94.36% overall alignment rate Time searching: 00:07:03 Overall time: 00:07:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2682651 / 19829757 = 0.1353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:16:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:16:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:16:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:16:43: 1000000 INFO @ Mon, 29 Jun 2020 23:16:49: 2000000 INFO @ Mon, 29 Jun 2020 23:16:55: 3000000 INFO @ Mon, 29 Jun 2020 23:17:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:17:06: 5000000 INFO @ Mon, 29 Jun 2020 23:17:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:17:07: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:17:07: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:17:13: 6000000 INFO @ Mon, 29 Jun 2020 23:17:14: 1000000 INFO @ Mon, 29 Jun 2020 23:17:20: 7000000 INFO @ Mon, 29 Jun 2020 23:17:20: 2000000 INFO @ Mon, 29 Jun 2020 23:17:26: 8000000 INFO @ Mon, 29 Jun 2020 23:17:27: 3000000 INFO @ Mon, 29 Jun 2020 23:17:33: 9000000 INFO @ Mon, 29 Jun 2020 23:17:34: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:17:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:17:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:17:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:17:40: 10000000 INFO @ Mon, 29 Jun 2020 23:17:41: 5000000 INFO @ Mon, 29 Jun 2020 23:17:45: 1000000 INFO @ Mon, 29 Jun 2020 23:17:47: 11000000 INFO @ Mon, 29 Jun 2020 23:17:48: 6000000 INFO @ Mon, 29 Jun 2020 23:17:53: 2000000 INFO @ Mon, 29 Jun 2020 23:17:54: 12000000 INFO @ Mon, 29 Jun 2020 23:17:55: 7000000 INFO @ Mon, 29 Jun 2020 23:18:00: 3000000 INFO @ Mon, 29 Jun 2020 23:18:02: 13000000 INFO @ Mon, 29 Jun 2020 23:18:03: 8000000 INFO @ Mon, 29 Jun 2020 23:18:08: 4000000 INFO @ Mon, 29 Jun 2020 23:18:09: 14000000 INFO @ Mon, 29 Jun 2020 23:18:10: 9000000 INFO @ Mon, 29 Jun 2020 23:18:16: 5000000 INFO @ Mon, 29 Jun 2020 23:18:16: 15000000 INFO @ Mon, 29 Jun 2020 23:18:17: 10000000 INFO @ Mon, 29 Jun 2020 23:18:23: 16000000 INFO @ Mon, 29 Jun 2020 23:18:24: 6000000 INFO @ Mon, 29 Jun 2020 23:18:24: 11000000 INFO @ Mon, 29 Jun 2020 23:18:31: 17000000 INFO @ Mon, 29 Jun 2020 23:18:31: 12000000 INFO @ Mon, 29 Jun 2020 23:18:32: 7000000 INFO @ Mon, 29 Jun 2020 23:18:32: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:18:32: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:18:32: #1 total tags in treatment: 17147106 INFO @ Mon, 29 Jun 2020 23:18:32: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:18:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:18:32: #1 tags after filtering in treatment: 17147106 INFO @ Mon, 29 Jun 2020 23:18:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:18:32: #1 finished! INFO @ Mon, 29 Jun 2020 23:18:32: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:18:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:18:33: #2 number of paired peaks: 67 WARNING @ Mon, 29 Jun 2020 23:18:33: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:18:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:18:39: 13000000 INFO @ Mon, 29 Jun 2020 23:18:40: 8000000 INFO @ Mon, 29 Jun 2020 23:18:46: 14000000 INFO @ Mon, 29 Jun 2020 23:18:47: 9000000 INFO @ Mon, 29 Jun 2020 23:18:54: 15000000 INFO @ Mon, 29 Jun 2020 23:18:55: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:19:01: 16000000 INFO @ Mon, 29 Jun 2020 23:19:03: 11000000 INFO @ Mon, 29 Jun 2020 23:19:09: 17000000 INFO @ Mon, 29 Jun 2020 23:19:10: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:19:10: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:19:10: #1 total tags in treatment: 17147106 INFO @ Mon, 29 Jun 2020 23:19:10: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:19:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:19:10: #1 tags after filtering in treatment: 17147106 INFO @ Mon, 29 Jun 2020 23:19:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:19:10: #1 finished! INFO @ Mon, 29 Jun 2020 23:19:10: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:19:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:19:11: 12000000 INFO @ Mon, 29 Jun 2020 23:19:11: #2 number of paired peaks: 67 WARNING @ Mon, 29 Jun 2020 23:19:11: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:19:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:19:19: 13000000 INFO @ Mon, 29 Jun 2020 23:19:26: 14000000 INFO @ Mon, 29 Jun 2020 23:19:34: 15000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:19:41: 16000000 INFO @ Mon, 29 Jun 2020 23:19:49: 17000000 INFO @ Mon, 29 Jun 2020 23:19:50: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:19:50: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:19:50: #1 total tags in treatment: 17147106 INFO @ Mon, 29 Jun 2020 23:19:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:19:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:19:50: #1 tags after filtering in treatment: 17147106 INFO @ Mon, 29 Jun 2020 23:19:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:19:50: #1 finished! INFO @ Mon, 29 Jun 2020 23:19:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:19:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:19:52: #2 number of paired peaks: 67 WARNING @ Mon, 29 Jun 2020 23:19:52: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:19:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318791/SRX318791.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling