Job ID = 6527914 SRX = SRX318790 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:44:41 prefetch.2.10.7: 1) Downloading 'SRR927106'... 2020-06-29T13:44:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:47:32 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:47:32 prefetch.2.10.7: 1) 'SRR927106' was downloaded successfully Read 17761171 spots for SRR927106/SRR927106.sra Written 17761171 spots for SRR927106/SRR927106.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:58 17761171 reads; of these: 17761171 (100.00%) were unpaired; of these: 964186 (5.43%) aligned 0 times 12931015 (72.80%) aligned exactly 1 time 3865970 (21.77%) aligned >1 times 94.57% overall alignment rate Time searching: 00:05:59 Overall time: 00:05:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1267214 / 16796985 = 0.0754 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:42: 1000000 INFO @ Mon, 29 Jun 2020 23:05:47: 2000000 INFO @ Mon, 29 Jun 2020 23:05:52: 3000000 INFO @ Mon, 29 Jun 2020 23:05:57: 4000000 INFO @ Mon, 29 Jun 2020 23:06:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:07: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:07: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:07: 6000000 INFO @ Mon, 29 Jun 2020 23:06:12: 1000000 INFO @ Mon, 29 Jun 2020 23:06:12: 7000000 INFO @ Mon, 29 Jun 2020 23:06:17: 2000000 INFO @ Mon, 29 Jun 2020 23:06:17: 8000000 INFO @ Mon, 29 Jun 2020 23:06:22: 3000000 INFO @ Mon, 29 Jun 2020 23:06:22: 9000000 INFO @ Mon, 29 Jun 2020 23:06:27: 10000000 INFO @ Mon, 29 Jun 2020 23:06:27: 4000000 INFO @ Mon, 29 Jun 2020 23:06:32: 11000000 INFO @ Mon, 29 Jun 2020 23:06:33: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:37: 12000000 INFO @ Mon, 29 Jun 2020 23:06:38: 6000000 INFO @ Mon, 29 Jun 2020 23:06:42: 1000000 INFO @ Mon, 29 Jun 2020 23:06:42: 13000000 INFO @ Mon, 29 Jun 2020 23:06:44: 7000000 INFO @ Mon, 29 Jun 2020 23:06:48: 14000000 INFO @ Mon, 29 Jun 2020 23:06:48: 2000000 INFO @ Mon, 29 Jun 2020 23:06:49: 8000000 INFO @ Mon, 29 Jun 2020 23:06:53: 15000000 INFO @ Mon, 29 Jun 2020 23:06:54: 3000000 INFO @ Mon, 29 Jun 2020 23:06:55: 9000000 INFO @ Mon, 29 Jun 2020 23:06:55: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:06:55: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:06:55: #1 total tags in treatment: 15529771 INFO @ Mon, 29 Jun 2020 23:06:55: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:06:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:06:56: #1 tags after filtering in treatment: 15529771 INFO @ Mon, 29 Jun 2020 23:06:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:06:56: #1 finished! INFO @ Mon, 29 Jun 2020 23:06:56: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:06:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:06:57: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:06:57: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:06:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:06:59: 4000000 INFO @ Mon, 29 Jun 2020 23:07:00: 10000000 INFO @ Mon, 29 Jun 2020 23:07:05: 5000000 INFO @ Mon, 29 Jun 2020 23:07:06: 11000000 INFO @ Mon, 29 Jun 2020 23:07:10: 6000000 INFO @ Mon, 29 Jun 2020 23:07:11: 12000000 INFO @ Mon, 29 Jun 2020 23:07:16: 7000000 INFO @ Mon, 29 Jun 2020 23:07:17: 13000000 INFO @ Mon, 29 Jun 2020 23:07:21: 8000000 INFO @ Mon, 29 Jun 2020 23:07:22: 14000000 INFO @ Mon, 29 Jun 2020 23:07:27: 9000000 INFO @ Mon, 29 Jun 2020 23:07:28: 15000000 INFO @ Mon, 29 Jun 2020 23:07:31: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:31: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:31: #1 total tags in treatment: 15529771 INFO @ Mon, 29 Jun 2020 23:07:31: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:31: #1 tags after filtering in treatment: 15529771 INFO @ Mon, 29 Jun 2020 23:07:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:31: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:31: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:32: 10000000 INFO @ Mon, 29 Jun 2020 23:07:32: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:07:32: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:07:37: 11000000 INFO @ Mon, 29 Jun 2020 23:07:42: 12000000 INFO @ Mon, 29 Jun 2020 23:07:48: 13000000 INFO @ Mon, 29 Jun 2020 23:07:53: 14000000 INFO @ Mon, 29 Jun 2020 23:07:58: 15000000 INFO @ Mon, 29 Jun 2020 23:08:01: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:01: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:01: #1 total tags in treatment: 15529771 INFO @ Mon, 29 Jun 2020 23:08:01: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:01: #1 tags after filtering in treatment: 15529771 INFO @ Mon, 29 Jun 2020 23:08:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:01: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:01: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:02: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:08:02: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318790/SRX318790.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。