Job ID = 6527913 SRX = SRX318786 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:43:11 prefetch.2.10.7: 1) Downloading 'SRR927102'... 2020-06-29T13:43:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:48:25 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:48:25 prefetch.2.10.7: 1) 'SRR927102' was downloaded successfully Read 18412477 spots for SRR927102/SRR927102.sra Written 18412477 spots for SRR927102/SRR927102.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:55 18412477 reads; of these: 18412477 (100.00%) were unpaired; of these: 986693 (5.36%) aligned 0 times 13569849 (73.70%) aligned exactly 1 time 3855935 (20.94%) aligned >1 times 94.64% overall alignment rate Time searching: 00:05:55 Overall time: 00:05:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1205054 / 17425784 = 0.0692 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:07:06: 1000000 INFO @ Mon, 29 Jun 2020 23:07:12: 2000000 INFO @ Mon, 29 Jun 2020 23:07:19: 3000000 INFO @ Mon, 29 Jun 2020 23:07:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:07:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:07:29: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:07:29: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:07:32: 5000000 INFO @ Mon, 29 Jun 2020 23:07:36: 1000000 INFO @ Mon, 29 Jun 2020 23:07:40: 6000000 INFO @ Mon, 29 Jun 2020 23:07:42: 2000000 INFO @ Mon, 29 Jun 2020 23:07:47: 7000000 INFO @ Mon, 29 Jun 2020 23:07:49: 3000000 INFO @ Mon, 29 Jun 2020 23:07:53: 8000000 INFO @ Mon, 29 Jun 2020 23:07:56: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:07:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:07:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:07:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:08:01: 9000000 INFO @ Mon, 29 Jun 2020 23:08:03: 5000000 INFO @ Mon, 29 Jun 2020 23:08:07: 1000000 INFO @ Mon, 29 Jun 2020 23:08:08: 10000000 INFO @ Mon, 29 Jun 2020 23:08:09: 6000000 INFO @ Mon, 29 Jun 2020 23:08:15: 2000000 INFO @ Mon, 29 Jun 2020 23:08:16: 11000000 INFO @ Mon, 29 Jun 2020 23:08:16: 7000000 INFO @ Mon, 29 Jun 2020 23:08:23: 3000000 INFO @ Mon, 29 Jun 2020 23:08:23: 12000000 INFO @ Mon, 29 Jun 2020 23:08:23: 8000000 INFO @ Mon, 29 Jun 2020 23:08:30: 9000000 INFO @ Mon, 29 Jun 2020 23:08:31: 4000000 INFO @ Mon, 29 Jun 2020 23:08:31: 13000000 INFO @ Mon, 29 Jun 2020 23:08:37: 10000000 INFO @ Mon, 29 Jun 2020 23:08:38: 5000000 INFO @ Mon, 29 Jun 2020 23:08:39: 14000000 INFO @ Mon, 29 Jun 2020 23:08:45: 11000000 INFO @ Mon, 29 Jun 2020 23:08:46: 6000000 INFO @ Mon, 29 Jun 2020 23:08:47: 15000000 INFO @ Mon, 29 Jun 2020 23:08:52: 12000000 INFO @ Mon, 29 Jun 2020 23:08:54: 7000000 INFO @ Mon, 29 Jun 2020 23:08:54: 16000000 INFO @ Mon, 29 Jun 2020 23:08:56: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:56: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:56: #1 total tags in treatment: 16220730 INFO @ Mon, 29 Jun 2020 23:08:56: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:57: #1 tags after filtering in treatment: 16220730 INFO @ Mon, 29 Jun 2020 23:08:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:57: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:57: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:58: #2 number of paired peaks: 52 WARNING @ Mon, 29 Jun 2020 23:08:58: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:08:59: 13000000 INFO @ Mon, 29 Jun 2020 23:09:01: 8000000 INFO @ Mon, 29 Jun 2020 23:09:06: 14000000 INFO @ Mon, 29 Jun 2020 23:09:08: 9000000 INFO @ Mon, 29 Jun 2020 23:09:12: 15000000 INFO @ Mon, 29 Jun 2020 23:09:15: 10000000 INFO @ Mon, 29 Jun 2020 23:09:19: 16000000 INFO @ Mon, 29 Jun 2020 23:09:21: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:09:21: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:09:21: #1 total tags in treatment: 16220730 INFO @ Mon, 29 Jun 2020 23:09:21: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:09:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:09:21: #1 tags after filtering in treatment: 16220730 INFO @ Mon, 29 Jun 2020 23:09:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:09:21: #1 finished! INFO @ Mon, 29 Jun 2020 23:09:21: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:09:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:09:22: #2 number of paired peaks: 52 WARNING @ Mon, 29 Jun 2020 23:09:22: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:09:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:09:22: 11000000 INFO @ Mon, 29 Jun 2020 23:09:29: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:09:36: 13000000 INFO @ Mon, 29 Jun 2020 23:09:42: 14000000 INFO @ Mon, 29 Jun 2020 23:09:49: 15000000 INFO @ Mon, 29 Jun 2020 23:09:56: 16000000 INFO @ Mon, 29 Jun 2020 23:09:57: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:09:57: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:09:57: #1 total tags in treatment: 16220730 INFO @ Mon, 29 Jun 2020 23:09:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:09:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:09:58: #1 tags after filtering in treatment: 16220730 INFO @ Mon, 29 Jun 2020 23:09:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:09:58: #1 finished! INFO @ Mon, 29 Jun 2020 23:09:58: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:09:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:09:59: #2 number of paired peaks: 52 WARNING @ Mon, 29 Jun 2020 23:09:59: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:09:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318786/SRX318786.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。