Job ID = 6527912 SRX = SRX318785 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:08:20 prefetch.2.10.7: 1) Downloading 'SRR927101'... 2020-06-29T14:08:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:11:52 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:11:52 prefetch.2.10.7: 1) 'SRR927101' was downloaded successfully Read 18006243 spots for SRR927101/SRR927101.sra Written 18006243 spots for SRR927101/SRR927101.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:45 18006243 reads; of these: 18006243 (100.00%) were unpaired; of these: 1173878 (6.52%) aligned 0 times 13082278 (72.65%) aligned exactly 1 time 3750087 (20.83%) aligned >1 times 93.48% overall alignment rate Time searching: 00:05:45 Overall time: 00:05:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1444300 / 16832365 = 0.0858 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:28:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:28:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:28:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:28:07: 1000000 INFO @ Mon, 29 Jun 2020 23:28:13: 2000000 INFO @ Mon, 29 Jun 2020 23:28:19: 3000000 INFO @ Mon, 29 Jun 2020 23:28:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:28:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:28:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:28:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:28:32: 5000000 INFO @ Mon, 29 Jun 2020 23:28:39: 1000000 INFO @ Mon, 29 Jun 2020 23:28:40: 6000000 INFO @ Mon, 29 Jun 2020 23:28:47: 2000000 INFO @ Mon, 29 Jun 2020 23:28:49: 7000000 INFO @ Mon, 29 Jun 2020 23:28:55: 3000000 INFO @ Mon, 29 Jun 2020 23:28:57: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:29:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:29:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:29:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:29:03: 4000000 INFO @ Mon, 29 Jun 2020 23:29:05: 9000000 INFO @ Mon, 29 Jun 2020 23:29:09: 1000000 INFO @ Mon, 29 Jun 2020 23:29:12: 5000000 INFO @ Mon, 29 Jun 2020 23:29:13: 10000000 INFO @ Mon, 29 Jun 2020 23:29:18: 2000000 INFO @ Mon, 29 Jun 2020 23:29:21: 6000000 INFO @ Mon, 29 Jun 2020 23:29:22: 11000000 INFO @ Mon, 29 Jun 2020 23:29:26: 3000000 INFO @ Mon, 29 Jun 2020 23:29:29: 7000000 INFO @ Mon, 29 Jun 2020 23:29:31: 12000000 INFO @ Mon, 29 Jun 2020 23:29:35: 4000000 INFO @ Mon, 29 Jun 2020 23:29:38: 8000000 INFO @ Mon, 29 Jun 2020 23:29:39: 13000000 INFO @ Mon, 29 Jun 2020 23:29:44: 5000000 INFO @ Mon, 29 Jun 2020 23:29:46: 9000000 INFO @ Mon, 29 Jun 2020 23:29:48: 14000000 INFO @ Mon, 29 Jun 2020 23:29:52: 6000000 INFO @ Mon, 29 Jun 2020 23:29:55: 10000000 INFO @ Mon, 29 Jun 2020 23:29:56: 15000000 INFO @ Mon, 29 Jun 2020 23:30:00: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:30:00: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:30:00: #1 total tags in treatment: 15388065 INFO @ Mon, 29 Jun 2020 23:30:00: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:30:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:30:00: #1 tags after filtering in treatment: 15388065 INFO @ Mon, 29 Jun 2020 23:30:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:30:00: #1 finished! INFO @ Mon, 29 Jun 2020 23:30:00: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:30:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:30:01: 7000000 INFO @ Mon, 29 Jun 2020 23:30:01: #2 number of paired peaks: 18 WARNING @ Mon, 29 Jun 2020 23:30:01: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:30:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:30:04: 11000000 INFO @ Mon, 29 Jun 2020 23:30:10: 8000000 INFO @ Mon, 29 Jun 2020 23:30:13: 12000000 INFO @ Mon, 29 Jun 2020 23:30:18: 9000000 INFO @ Mon, 29 Jun 2020 23:30:21: 13000000 INFO @ Mon, 29 Jun 2020 23:30:27: 10000000 INFO @ Mon, 29 Jun 2020 23:30:30: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:30:36: 11000000 INFO @ Mon, 29 Jun 2020 23:30:39: 15000000 INFO @ Mon, 29 Jun 2020 23:30:42: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:30:42: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:30:42: #1 total tags in treatment: 15388065 INFO @ Mon, 29 Jun 2020 23:30:42: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:30:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:30:42: #1 tags after filtering in treatment: 15388065 INFO @ Mon, 29 Jun 2020 23:30:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:30:42: #1 finished! INFO @ Mon, 29 Jun 2020 23:30:42: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:30:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:30:43: #2 number of paired peaks: 18 WARNING @ Mon, 29 Jun 2020 23:30:43: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:30:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:30:44: 12000000 INFO @ Mon, 29 Jun 2020 23:30:52: 13000000 INFO @ Mon, 29 Jun 2020 23:31:00: 14000000 INFO @ Mon, 29 Jun 2020 23:31:08: 15000000 INFO @ Mon, 29 Jun 2020 23:31:11: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:31:11: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:31:11: #1 total tags in treatment: 15388065 INFO @ Mon, 29 Jun 2020 23:31:11: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:31:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:31:11: #1 tags after filtering in treatment: 15388065 INFO @ Mon, 29 Jun 2020 23:31:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:31:11: #1 finished! INFO @ Mon, 29 Jun 2020 23:31:11: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:31:11: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:31:12: #2 number of paired peaks: 18 WARNING @ Mon, 29 Jun 2020 23:31:12: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:31:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX318785/SRX318785.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling