Job ID = 10320337 sra ファイルのダウンロード中... Completed: 853455K bytes transferred in 12 seconds (574427K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 52409949 spots for /home/okishinya/chipatlas/results/dm3/SRX3166313/SRR6012930.sra Written 52409949 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:10 52409949 reads; of these: 52409949 (100.00%) were unpaired; of these: 1947782 (3.72%) aligned 0 times 41127787 (78.47%) aligned exactly 1 time 9334380 (17.81%) aligned >1 times 96.28% overall alignment rate Time searching: 00:16:11 Overall time: 00:16:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 6401197 / 50462167 = 0.1269 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 11 Jan 2018 02:34:15: # Command line: callpeak -t SRX3166313.bam -f BAM -g dm -n SRX3166313.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3166313.05 # format = BAM # ChIP-seq file = ['SRX3166313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:34:15: # Command line: callpeak -t SRX3166313.bam -f BAM -g dm -n SRX3166313.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3166313.20 # format = BAM # ChIP-seq file = ['SRX3166313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:34:15: # Command line: callpeak -t SRX3166313.bam -f BAM -g dm -n SRX3166313.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3166313.10 # format = BAM # ChIP-seq file = ['SRX3166313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:34:15: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:34:15: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:34:15: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:34:15: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:34:15: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:34:15: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:34:21: 1000000 INFO @ Thu, 11 Jan 2018 02:34:21: 1000000 INFO @ Thu, 11 Jan 2018 02:34:21: 1000000 INFO @ Thu, 11 Jan 2018 02:34:27: 2000000 INFO @ Thu, 11 Jan 2018 02:34:27: 2000000 INFO @ Thu, 11 Jan 2018 02:34:27: 2000000 INFO @ Thu, 11 Jan 2018 02:34:33: 3000000 INFO @ Thu, 11 Jan 2018 02:34:33: 3000000 INFO @ Thu, 11 Jan 2018 02:34:33: 3000000 INFO @ Thu, 11 Jan 2018 02:34:39: 4000000 INFO @ Thu, 11 Jan 2018 02:34:39: 4000000 INFO @ Thu, 11 Jan 2018 02:34:40: 4000000 INFO @ Thu, 11 Jan 2018 02:34:45: 5000000 INFO @ Thu, 11 Jan 2018 02:34:46: 5000000 INFO @ Thu, 11 Jan 2018 02:34:46: 5000000 INFO @ Thu, 11 Jan 2018 02:34:51: 6000000 INFO @ Thu, 11 Jan 2018 02:34:52: 6000000 INFO @ Thu, 11 Jan 2018 02:34:53: 6000000 INFO @ Thu, 11 Jan 2018 02:34:57: 7000000 INFO @ Thu, 11 Jan 2018 02:34:58: 7000000 INFO @ Thu, 11 Jan 2018 02:35:00: 7000000 INFO @ Thu, 11 Jan 2018 02:35:03: 8000000 INFO @ Thu, 11 Jan 2018 02:35:05: 8000000 INFO @ Thu, 11 Jan 2018 02:35:06: 8000000 INFO @ Thu, 11 Jan 2018 02:35:09: 9000000 INFO @ Thu, 11 Jan 2018 02:35:11: 9000000 INFO @ Thu, 11 Jan 2018 02:35:13: 9000000 INFO @ Thu, 11 Jan 2018 02:35:15: 10000000 INFO @ Thu, 11 Jan 2018 02:35:17: 10000000 INFO @ Thu, 11 Jan 2018 02:35:20: 10000000 INFO @ Thu, 11 Jan 2018 02:35:21: 11000000 INFO @ Thu, 11 Jan 2018 02:35:24: 11000000 INFO @ Thu, 11 Jan 2018 02:35:26: 11000000 INFO @ Thu, 11 Jan 2018 02:35:27: 12000000 INFO @ Thu, 11 Jan 2018 02:35:30: 12000000 INFO @ Thu, 11 Jan 2018 02:35:33: 12000000 INFO @ Thu, 11 Jan 2018 02:35:33: 13000000 INFO @ Thu, 11 Jan 2018 02:35:36: 13000000 INFO @ Thu, 11 Jan 2018 02:35:39: 14000000 INFO @ Thu, 11 Jan 2018 02:35:40: 13000000 INFO @ Thu, 11 Jan 2018 02:35:43: 14000000 INFO @ Thu, 11 Jan 2018 02:35:45: 15000000 INFO @ Thu, 11 Jan 2018 02:35:47: 14000000 INFO @ Thu, 11 Jan 2018 02:35:50: 15000000 INFO @ Thu, 11 Jan 2018 02:35:51: 16000000 INFO @ Thu, 11 Jan 2018 02:35:54: 15000000 INFO @ Thu, 11 Jan 2018 02:35:56: 16000000 INFO @ Thu, 11 Jan 2018 02:35:57: 17000000 INFO @ Thu, 11 Jan 2018 02:36:00: 16000000 INFO @ Thu, 11 Jan 2018 02:36:02: 17000000 INFO @ Thu, 11 Jan 2018 02:36:03: 18000000 INFO @ Thu, 11 Jan 2018 02:36:07: 17000000 INFO @ Thu, 11 Jan 2018 02:36:09: 18000000 INFO @ Thu, 11 Jan 2018 02:36:09: 19000000 INFO @ Thu, 11 Jan 2018 02:36:14: 18000000 INFO @ Thu, 11 Jan 2018 02:36:15: 20000000 INFO @ Thu, 11 Jan 2018 02:36:15: 19000000 INFO @ Thu, 11 Jan 2018 02:36:20: 19000000 INFO @ Thu, 11 Jan 2018 02:36:21: 21000000 INFO @ Thu, 11 Jan 2018 02:36:21: 20000000 INFO @ Thu, 11 Jan 2018 02:36:27: 20000000 INFO @ Thu, 11 Jan 2018 02:36:27: 22000000 INFO @ Thu, 11 Jan 2018 02:36:28: 21000000 INFO @ Thu, 11 Jan 2018 02:36:33: 23000000 INFO @ Thu, 11 Jan 2018 02:36:33: 21000000 INFO @ Thu, 11 Jan 2018 02:36:34: 22000000 INFO @ Thu, 11 Jan 2018 02:36:39: 24000000 INFO @ Thu, 11 Jan 2018 02:36:40: 22000000 INFO @ Thu, 11 Jan 2018 02:36:40: 23000000 INFO @ Thu, 11 Jan 2018 02:36:45: 25000000 INFO @ Thu, 11 Jan 2018 02:36:46: 23000000 INFO @ Thu, 11 Jan 2018 02:36:46: 24000000 INFO @ Thu, 11 Jan 2018 02:36:51: 26000000 INFO @ Thu, 11 Jan 2018 02:36:53: 25000000 INFO @ Thu, 11 Jan 2018 02:36:53: 24000000 INFO @ Thu, 11 Jan 2018 02:36:57: 27000000 INFO @ Thu, 11 Jan 2018 02:36:59: 26000000 INFO @ Thu, 11 Jan 2018 02:36:59: 25000000 INFO @ Thu, 11 Jan 2018 02:37:03: 28000000 INFO @ Thu, 11 Jan 2018 02:37:05: 27000000 INFO @ Thu, 11 Jan 2018 02:37:06: 26000000 INFO @ Thu, 11 Jan 2018 02:37:09: 29000000 INFO @ Thu, 11 Jan 2018 02:37:12: 28000000 INFO @ Thu, 11 Jan 2018 02:37:14: 27000000 INFO @ Thu, 11 Jan 2018 02:37:15: 30000000 INFO @ Thu, 11 Jan 2018 02:37:19: 29000000 INFO @ Thu, 11 Jan 2018 02:37:21: 31000000 INFO @ Thu, 11 Jan 2018 02:37:22: 28000000 INFO @ Thu, 11 Jan 2018 02:37:27: 30000000 INFO @ Thu, 11 Jan 2018 02:37:28: 32000000 INFO @ Thu, 11 Jan 2018 02:37:30: 29000000 INFO @ Thu, 11 Jan 2018 02:37:34: 33000000 INFO @ Thu, 11 Jan 2018 02:37:34: 31000000 INFO @ Thu, 11 Jan 2018 02:37:38: 30000000 INFO @ Thu, 11 Jan 2018 02:37:40: 34000000 INFO @ Thu, 11 Jan 2018 02:37:41: 32000000 INFO @ Thu, 11 Jan 2018 02:37:46: 31000000 INFO @ Thu, 11 Jan 2018 02:37:46: 35000000 INFO @ Thu, 11 Jan 2018 02:37:49: 33000000 INFO @ Thu, 11 Jan 2018 02:37:53: 36000000 INFO @ Thu, 11 Jan 2018 02:37:54: 32000000 INFO @ Thu, 11 Jan 2018 02:37:56: 34000000 INFO @ Thu, 11 Jan 2018 02:37:59: 37000000 INFO @ Thu, 11 Jan 2018 02:38:02: 33000000 INFO @ Thu, 11 Jan 2018 02:38:04: 35000000 INFO @ Thu, 11 Jan 2018 02:38:05: 38000000 INFO @ Thu, 11 Jan 2018 02:38:10: 34000000 INFO @ Thu, 11 Jan 2018 02:38:11: 36000000 INFO @ Thu, 11 Jan 2018 02:38:12: 39000000 INFO @ Thu, 11 Jan 2018 02:38:18: 40000000 INFO @ Thu, 11 Jan 2018 02:38:18: 35000000 INFO @ Thu, 11 Jan 2018 02:38:19: 37000000 INFO @ Thu, 11 Jan 2018 02:38:25: 41000000 INFO @ Thu, 11 Jan 2018 02:38:26: 38000000 INFO @ Thu, 11 Jan 2018 02:38:27: 36000000 INFO @ Thu, 11 Jan 2018 02:38:31: 42000000 INFO @ Thu, 11 Jan 2018 02:38:34: 39000000 INFO @ Thu, 11 Jan 2018 02:38:35: 37000000 INFO @ Thu, 11 Jan 2018 02:38:37: 43000000 INFO @ Thu, 11 Jan 2018 02:38:41: 40000000 INFO @ Thu, 11 Jan 2018 02:38:43: 38000000 INFO @ Thu, 11 Jan 2018 02:38:44: 44000000 INFO @ Thu, 11 Jan 2018 02:38:44: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:38:44: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:38:44: #1 total tags in treatment: 44060970 INFO @ Thu, 11 Jan 2018 02:38:44: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:38:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:38:45: #1 tags after filtering in treatment: 44060970 INFO @ Thu, 11 Jan 2018 02:38:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:38:45: #1 finished! INFO @ Thu, 11 Jan 2018 02:38:45: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:38:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:38:48: #2 number of paired peaks: 33 WARNING @ Thu, 11 Jan 2018 02:38:48: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:38:48: Process for pairing-model is terminated! cat: SRX3166313.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3166313.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166313.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166313.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 11 Jan 2018 02:38:49: 41000000 INFO @ Thu, 11 Jan 2018 02:38:51: 39000000 INFO @ Thu, 11 Jan 2018 02:38:56: 42000000 INFO @ Thu, 11 Jan 2018 02:38:57: 40000000 INFO @ Thu, 11 Jan 2018 02:39:03: 43000000 INFO @ Thu, 11 Jan 2018 02:39:04: 41000000 INFO @ Thu, 11 Jan 2018 02:39:10: 44000000 INFO @ Thu, 11 Jan 2018 02:39:10: 42000000 INFO @ Thu, 11 Jan 2018 02:39:10: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:39:10: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:39:10: #1 total tags in treatment: 44060970 INFO @ Thu, 11 Jan 2018 02:39:10: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:39:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:39:11: #1 tags after filtering in treatment: 44060970 INFO @ Thu, 11 Jan 2018 02:39:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:39:11: #1 finished! INFO @ Thu, 11 Jan 2018 02:39:11: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:39:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:39:14: #2 number of paired peaks: 33 WARNING @ Thu, 11 Jan 2018 02:39:14: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:39:14: Process for pairing-model is terminated! cat: SRX3166313.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3166313.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166313.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166313.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 11 Jan 2018 02:39:16: 43000000 INFO @ Thu, 11 Jan 2018 02:39:24: 44000000 INFO @ Thu, 11 Jan 2018 02:39:24: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:39:24: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:39:24: #1 total tags in treatment: 44060970 INFO @ Thu, 11 Jan 2018 02:39:24: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:39:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:39:25: #1 tags after filtering in treatment: 44060970 INFO @ Thu, 11 Jan 2018 02:39:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:39:25: #1 finished! INFO @ Thu, 11 Jan 2018 02:39:25: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:39:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:39:28: #2 number of paired peaks: 33 WARNING @ Thu, 11 Jan 2018 02:39:28: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:39:28: Process for pairing-model is terminated! cat: SRX3166313.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3166313.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166313.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166313.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。