Job ID = 10320336 sra ファイルのダウンロード中... Completed: 934450K bytes transferred in 12 seconds (633472K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 57825933 spots for /home/okishinya/chipatlas/results/dm3/SRX3166312/SRR6012929.sra Written 57825933 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:44 57825933 reads; of these: 57825933 (100.00%) were unpaired; of these: 2133315 (3.69%) aligned 0 times 45558997 (78.79%) aligned exactly 1 time 10133621 (17.52%) aligned >1 times 96.31% overall alignment rate Time searching: 00:17:44 Overall time: 00:17:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 7141626 / 55692618 = 0.1282 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 11 Jan 2018 02:37:07: # Command line: callpeak -t SRX3166312.bam -f BAM -g dm -n SRX3166312.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3166312.10 # format = BAM # ChIP-seq file = ['SRX3166312.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:37:07: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:37:07: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:37:07: # Command line: callpeak -t SRX3166312.bam -f BAM -g dm -n SRX3166312.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3166312.05 # format = BAM # ChIP-seq file = ['SRX3166312.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:37:07: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:37:07: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:37:07: # Command line: callpeak -t SRX3166312.bam -f BAM -g dm -n SRX3166312.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3166312.20 # format = BAM # ChIP-seq file = ['SRX3166312.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 11 Jan 2018 02:37:07: #1 read tag files... INFO @ Thu, 11 Jan 2018 02:37:07: #1 read treatment tags... INFO @ Thu, 11 Jan 2018 02:37:13: 1000000 INFO @ Thu, 11 Jan 2018 02:37:14: 1000000 INFO @ Thu, 11 Jan 2018 02:37:14: 1000000 INFO @ Thu, 11 Jan 2018 02:37:20: 2000000 INFO @ Thu, 11 Jan 2018 02:37:21: 2000000 INFO @ Thu, 11 Jan 2018 02:37:21: 2000000 INFO @ Thu, 11 Jan 2018 02:37:26: 3000000 INFO @ Thu, 11 Jan 2018 02:37:28: 3000000 INFO @ Thu, 11 Jan 2018 02:37:28: 3000000 INFO @ Thu, 11 Jan 2018 02:37:32: 4000000 INFO @ Thu, 11 Jan 2018 02:37:35: 4000000 INFO @ Thu, 11 Jan 2018 02:37:35: 4000000 INFO @ Thu, 11 Jan 2018 02:37:38: 5000000 INFO @ Thu, 11 Jan 2018 02:37:42: 5000000 INFO @ Thu, 11 Jan 2018 02:37:42: 5000000 INFO @ Thu, 11 Jan 2018 02:37:44: 6000000 INFO @ Thu, 11 Jan 2018 02:37:49: 6000000 INFO @ Thu, 11 Jan 2018 02:37:49: 6000000 INFO @ Thu, 11 Jan 2018 02:37:51: 7000000 INFO @ Thu, 11 Jan 2018 02:37:56: 7000000 INFO @ Thu, 11 Jan 2018 02:37:56: 7000000 INFO @ Thu, 11 Jan 2018 02:37:57: 8000000 INFO @ Thu, 11 Jan 2018 02:38:03: 8000000 INFO @ Thu, 11 Jan 2018 02:38:03: 8000000 INFO @ Thu, 11 Jan 2018 02:38:03: 9000000 INFO @ Thu, 11 Jan 2018 02:38:09: 10000000 INFO @ Thu, 11 Jan 2018 02:38:10: 9000000 INFO @ Thu, 11 Jan 2018 02:38:10: 9000000 INFO @ Thu, 11 Jan 2018 02:38:15: 11000000 INFO @ Thu, 11 Jan 2018 02:38:17: 10000000 INFO @ Thu, 11 Jan 2018 02:38:17: 10000000 INFO @ Thu, 11 Jan 2018 02:38:22: 12000000 INFO @ Thu, 11 Jan 2018 02:38:24: 11000000 INFO @ Thu, 11 Jan 2018 02:38:24: 11000000 INFO @ Thu, 11 Jan 2018 02:38:28: 13000000 INFO @ Thu, 11 Jan 2018 02:38:31: 12000000 INFO @ Thu, 11 Jan 2018 02:38:31: 12000000 INFO @ Thu, 11 Jan 2018 02:38:34: 14000000 INFO @ Thu, 11 Jan 2018 02:38:38: 13000000 INFO @ Thu, 11 Jan 2018 02:38:38: 13000000 INFO @ Thu, 11 Jan 2018 02:38:40: 15000000 INFO @ Thu, 11 Jan 2018 02:38:44: 14000000 INFO @ Thu, 11 Jan 2018 02:38:44: 14000000 INFO @ Thu, 11 Jan 2018 02:38:46: 16000000 INFO @ Thu, 11 Jan 2018 02:38:51: 15000000 INFO @ Thu, 11 Jan 2018 02:38:51: 15000000 INFO @ Thu, 11 Jan 2018 02:38:52: 17000000 INFO @ Thu, 11 Jan 2018 02:38:58: 16000000 INFO @ Thu, 11 Jan 2018 02:38:58: 16000000 INFO @ Thu, 11 Jan 2018 02:38:58: 18000000 INFO @ Thu, 11 Jan 2018 02:39:04: 17000000 INFO @ Thu, 11 Jan 2018 02:39:04: 19000000 INFO @ Thu, 11 Jan 2018 02:39:04: 17000000 INFO @ Thu, 11 Jan 2018 02:39:10: 20000000 INFO @ Thu, 11 Jan 2018 02:39:11: 18000000 INFO @ Thu, 11 Jan 2018 02:39:11: 18000000 INFO @ Thu, 11 Jan 2018 02:39:16: 21000000 INFO @ Thu, 11 Jan 2018 02:39:17: 19000000 INFO @ Thu, 11 Jan 2018 02:39:17: 19000000 INFO @ Thu, 11 Jan 2018 02:39:22: 22000000 INFO @ Thu, 11 Jan 2018 02:39:23: 20000000 INFO @ Thu, 11 Jan 2018 02:39:24: 20000000 INFO @ Thu, 11 Jan 2018 02:39:28: 23000000 INFO @ Thu, 11 Jan 2018 02:39:29: 21000000 INFO @ Thu, 11 Jan 2018 02:39:30: 21000000 INFO @ Thu, 11 Jan 2018 02:39:34: 24000000 INFO @ Thu, 11 Jan 2018 02:39:35: 22000000 INFO @ Thu, 11 Jan 2018 02:39:36: 22000000 INFO @ Thu, 11 Jan 2018 02:39:39: 25000000 INFO @ Thu, 11 Jan 2018 02:39:41: 23000000 INFO @ Thu, 11 Jan 2018 02:39:42: 23000000 INFO @ Thu, 11 Jan 2018 02:39:45: 26000000 INFO @ Thu, 11 Jan 2018 02:39:47: 24000000 INFO @ Thu, 11 Jan 2018 02:39:49: 24000000 INFO @ Thu, 11 Jan 2018 02:39:51: 27000000 INFO @ Thu, 11 Jan 2018 02:39:54: 25000000 INFO @ Thu, 11 Jan 2018 02:39:55: 25000000 INFO @ Thu, 11 Jan 2018 02:39:56: 28000000 INFO @ Thu, 11 Jan 2018 02:40:00: 26000000 INFO @ Thu, 11 Jan 2018 02:40:01: 26000000 INFO @ Thu, 11 Jan 2018 02:40:02: 29000000 INFO @ Thu, 11 Jan 2018 02:40:06: 27000000 INFO @ Thu, 11 Jan 2018 02:40:07: 27000000 INFO @ Thu, 11 Jan 2018 02:40:08: 30000000 INFO @ Thu, 11 Jan 2018 02:40:12: 28000000 INFO @ Thu, 11 Jan 2018 02:40:13: 31000000 INFO @ Thu, 11 Jan 2018 02:40:14: 28000000 INFO @ Thu, 11 Jan 2018 02:40:18: 29000000 INFO @ Thu, 11 Jan 2018 02:40:19: 32000000 INFO @ Thu, 11 Jan 2018 02:40:20: 29000000 INFO @ Thu, 11 Jan 2018 02:40:24: 30000000 INFO @ Thu, 11 Jan 2018 02:40:25: 33000000 INFO @ Thu, 11 Jan 2018 02:40:26: 30000000 INFO @ Thu, 11 Jan 2018 02:40:30: 34000000 INFO @ Thu, 11 Jan 2018 02:40:31: 31000000 INFO @ Thu, 11 Jan 2018 02:40:32: 31000000 INFO @ Thu, 11 Jan 2018 02:40:36: 35000000 INFO @ Thu, 11 Jan 2018 02:40:37: 32000000 INFO @ Thu, 11 Jan 2018 02:40:39: 32000000 INFO @ Thu, 11 Jan 2018 02:40:42: 36000000 INFO @ Thu, 11 Jan 2018 02:40:43: 33000000 INFO @ Thu, 11 Jan 2018 02:40:45: 33000000 INFO @ Thu, 11 Jan 2018 02:40:47: 37000000 INFO @ Thu, 11 Jan 2018 02:40:49: 34000000 INFO @ Thu, 11 Jan 2018 02:40:51: 34000000 INFO @ Thu, 11 Jan 2018 02:40:53: 38000000 INFO @ Thu, 11 Jan 2018 02:40:55: 35000000 INFO @ Thu, 11 Jan 2018 02:40:57: 35000000 INFO @ Thu, 11 Jan 2018 02:40:59: 39000000 INFO @ Thu, 11 Jan 2018 02:41:01: 36000000 INFO @ Thu, 11 Jan 2018 02:41:04: 36000000 INFO @ Thu, 11 Jan 2018 02:41:05: 40000000 INFO @ Thu, 11 Jan 2018 02:41:07: 37000000 INFO @ Thu, 11 Jan 2018 02:41:10: 37000000 INFO @ Thu, 11 Jan 2018 02:41:10: 41000000 INFO @ Thu, 11 Jan 2018 02:41:14: 38000000 INFO @ Thu, 11 Jan 2018 02:41:16: 42000000 INFO @ Thu, 11 Jan 2018 02:41:16: 38000000 INFO @ Thu, 11 Jan 2018 02:41:20: 39000000 INFO @ Thu, 11 Jan 2018 02:41:22: 43000000 INFO @ Thu, 11 Jan 2018 02:41:23: 39000000 INFO @ Thu, 11 Jan 2018 02:41:26: 40000000 INFO @ Thu, 11 Jan 2018 02:41:27: 44000000 INFO @ Thu, 11 Jan 2018 02:41:29: 40000000 INFO @ Thu, 11 Jan 2018 02:41:32: 41000000 INFO @ Thu, 11 Jan 2018 02:41:33: 45000000 INFO @ Thu, 11 Jan 2018 02:41:35: 41000000 INFO @ Thu, 11 Jan 2018 02:41:38: 42000000 INFO @ Thu, 11 Jan 2018 02:41:39: 46000000 INFO @ Thu, 11 Jan 2018 02:41:42: 42000000 INFO @ Thu, 11 Jan 2018 02:41:44: 47000000 INFO @ Thu, 11 Jan 2018 02:41:44: 43000000 INFO @ Thu, 11 Jan 2018 02:41:48: 43000000 INFO @ Thu, 11 Jan 2018 02:41:50: 48000000 INFO @ Thu, 11 Jan 2018 02:41:51: 44000000 INFO @ Thu, 11 Jan 2018 02:41:53: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:41:53: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:41:53: #1 total tags in treatment: 48550992 INFO @ Thu, 11 Jan 2018 02:41:53: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:41:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:41:54: 44000000 INFO @ Thu, 11 Jan 2018 02:41:55: #1 tags after filtering in treatment: 48550992 INFO @ Thu, 11 Jan 2018 02:41:55: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:41:55: #1 finished! INFO @ Thu, 11 Jan 2018 02:41:55: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:41:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:41:57: 45000000 INFO @ Thu, 11 Jan 2018 02:41:58: #2 number of paired peaks: 0 WARNING @ Thu, 11 Jan 2018 02:41:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:41:58: Process for pairing-model is terminated! cat: SRX3166312.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3166312.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166312.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166312.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 11 Jan 2018 02:42:00: 45000000 INFO @ Thu, 11 Jan 2018 02:42:03: 46000000 INFO @ Thu, 11 Jan 2018 02:42:07: 46000000 INFO @ Thu, 11 Jan 2018 02:42:09: 47000000 INFO @ Thu, 11 Jan 2018 02:42:13: 47000000 INFO @ Thu, 11 Jan 2018 02:42:15: 48000000 INFO @ Thu, 11 Jan 2018 02:42:19: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:42:19: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:42:19: #1 total tags in treatment: 48550992 INFO @ Thu, 11 Jan 2018 02:42:19: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:42:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:42:19: 48000000 INFO @ Thu, 11 Jan 2018 02:42:20: #1 tags after filtering in treatment: 48550992 INFO @ Thu, 11 Jan 2018 02:42:20: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:42:20: #1 finished! INFO @ Thu, 11 Jan 2018 02:42:20: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:42:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:42:23: #1 tag size is determined as 51 bps INFO @ Thu, 11 Jan 2018 02:42:23: #1 tag size = 51 INFO @ Thu, 11 Jan 2018 02:42:23: #1 total tags in treatment: 48550992 INFO @ Thu, 11 Jan 2018 02:42:23: #1 user defined the maximum tags... INFO @ Thu, 11 Jan 2018 02:42:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 11 Jan 2018 02:42:23: #2 number of paired peaks: 0 WARNING @ Thu, 11 Jan 2018 02:42:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:42:23: Process for pairing-model is terminated! cat: SRX3166312.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3166312.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166312.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166312.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 11 Jan 2018 02:42:24: #1 tags after filtering in treatment: 48550992 INFO @ Thu, 11 Jan 2018 02:42:24: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 11 Jan 2018 02:42:24: #1 finished! INFO @ Thu, 11 Jan 2018 02:42:24: #2 Build Peak Model... INFO @ Thu, 11 Jan 2018 02:42:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 11 Jan 2018 02:42:27: #2 number of paired peaks: 0 WARNING @ Thu, 11 Jan 2018 02:42:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 11 Jan 2018 02:42:27: Process for pairing-model is terminated! cat: SRX3166312.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3166312.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166312.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3166312.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。