Job ID = 12265176 SRX = SRX3141076 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 75627865 spots for SRR5985082/SRR5985082.sra Written 75627865 spots for SRR5985082/SRR5985082.sra fastq に変換しました。 bowtie でマッピング中... Your job 12266912 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:39:47 75627865 reads; of these: 75627865 (100.00%) were paired; of these: 25620315 (33.88%) aligned concordantly 0 times 22356093 (29.56%) aligned concordantly exactly 1 time 27651457 (36.56%) aligned concordantly >1 times ---- 25620315 pairs aligned concordantly 0 times; of these: 5375427 (20.98%) aligned discordantly 1 time ---- 20244888 pairs aligned 0 times concordantly or discordantly; of these: 40489776 mates make up the pairs; of these: 28063912 (69.31%) aligned 0 times 2050833 (5.07%) aligned exactly 1 time 10375031 (25.62%) aligned >1 times 81.45% overall alignment rate Time searching: 02:39:47 Overall time: 02:39:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 27109243 / 54736095 = 0.4953 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:07:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:07:29: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:07:29: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:07:38: 1000000 INFO @ Sat, 03 Apr 2021 10:07:48: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:07:57: 3000000 INFO @ Sat, 03 Apr 2021 10:07:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:07:59: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:07:59: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:08:06: 1000000 INFO @ Sat, 03 Apr 2021 10:08:07: 4000000 INFO @ Sat, 03 Apr 2021 10:08:13: 2000000 INFO @ Sat, 03 Apr 2021 10:08:16: 5000000 INFO @ Sat, 03 Apr 2021 10:08:20: 3000000 INFO @ Sat, 03 Apr 2021 10:08:26: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 10:08:28: 4000000 INFO @ Sat, 03 Apr 2021 10:08:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 10:08:29: #1 read tag files... INFO @ Sat, 03 Apr 2021 10:08:29: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 10:08:35: 5000000 INFO @ Sat, 03 Apr 2021 10:08:36: 7000000 INFO @ Sat, 03 Apr 2021 10:08:39: 1000000 INFO @ Sat, 03 Apr 2021 10:08:43: 6000000 INFO @ Sat, 03 Apr 2021 10:08:46: 8000000 INFO @ Sat, 03 Apr 2021 10:08:49: 2000000 INFO @ Sat, 03 Apr 2021 10:08:50: 7000000 INFO @ Sat, 03 Apr 2021 10:08:56: 9000000 INFO @ Sat, 03 Apr 2021 10:08:57: 8000000 INFO @ Sat, 03 Apr 2021 10:08:59: 3000000 INFO @ Sat, 03 Apr 2021 10:09:04: 9000000 INFO @ Sat, 03 Apr 2021 10:09:06: 10000000 INFO @ Sat, 03 Apr 2021 10:09:09: 4000000 INFO @ Sat, 03 Apr 2021 10:09:11: 10000000 INFO @ Sat, 03 Apr 2021 10:09:16: 11000000 INFO @ Sat, 03 Apr 2021 10:09:18: 11000000 INFO @ Sat, 03 Apr 2021 10:09:19: 5000000 INFO @ Sat, 03 Apr 2021 10:09:26: 12000000 INFO @ Sat, 03 Apr 2021 10:09:26: 12000000 INFO @ Sat, 03 Apr 2021 10:09:29: 6000000 INFO @ Sat, 03 Apr 2021 10:09:33: 13000000 INFO @ Sat, 03 Apr 2021 10:09:37: 13000000 INFO @ Sat, 03 Apr 2021 10:09:40: 7000000 INFO @ Sat, 03 Apr 2021 10:09:40: 14000000 INFO @ Sat, 03 Apr 2021 10:09:47: 14000000 INFO @ Sat, 03 Apr 2021 10:09:48: 15000000 INFO @ Sat, 03 Apr 2021 10:09:50: 8000000 INFO @ Sat, 03 Apr 2021 10:09:55: 16000000 INFO @ Sat, 03 Apr 2021 10:09:57: 15000000 INFO @ Sat, 03 Apr 2021 10:10:00: 9000000 INFO @ Sat, 03 Apr 2021 10:10:02: 17000000 INFO @ Sat, 03 Apr 2021 10:10:07: 16000000 INFO @ Sat, 03 Apr 2021 10:10:11: 10000000 INFO @ Sat, 03 Apr 2021 10:10:16: 18000000 INFO @ Sat, 03 Apr 2021 10:10:18: 17000000 INFO @ Sat, 03 Apr 2021 10:10:21: 11000000 INFO @ Sat, 03 Apr 2021 10:10:24: 19000000 INFO @ Sat, 03 Apr 2021 10:10:28: 18000000 INFO @ Sat, 03 Apr 2021 10:10:31: 20000000 INFO @ Sat, 03 Apr 2021 10:10:31: 12000000 INFO @ Sat, 03 Apr 2021 10:10:38: 21000000 INFO @ Sat, 03 Apr 2021 10:10:38: 19000000 INFO @ Sat, 03 Apr 2021 10:10:42: 13000000 INFO @ Sat, 03 Apr 2021 10:10:46: 22000000 INFO @ Sat, 03 Apr 2021 10:10:49: 20000000 INFO @ Sat, 03 Apr 2021 10:10:52: 14000000 INFO @ Sat, 03 Apr 2021 10:10:53: 23000000 INFO @ Sat, 03 Apr 2021 10:10:59: 21000000 INFO @ Sat, 03 Apr 2021 10:11:00: 24000000 INFO @ Sat, 03 Apr 2021 10:11:03: 15000000 INFO @ Sat, 03 Apr 2021 10:11:07: 25000000 INFO @ Sat, 03 Apr 2021 10:11:09: 22000000 INFO @ Sat, 03 Apr 2021 10:11:13: 16000000 INFO @ Sat, 03 Apr 2021 10:11:15: 26000000 INFO @ Sat, 03 Apr 2021 10:11:19: 23000000 INFO @ Sat, 03 Apr 2021 10:11:22: 27000000 INFO @ Sat, 03 Apr 2021 10:11:23: 17000000 INFO @ Sat, 03 Apr 2021 10:11:29: 24000000 INFO @ Sat, 03 Apr 2021 10:11:29: 28000000 INFO @ Sat, 03 Apr 2021 10:11:33: 18000000 INFO @ Sat, 03 Apr 2021 10:11:36: 29000000 INFO @ Sat, 03 Apr 2021 10:11:38: 25000000 INFO @ Sat, 03 Apr 2021 10:11:41: 19000000 INFO @ Sat, 03 Apr 2021 10:11:43: 30000000 INFO @ Sat, 03 Apr 2021 10:11:47: 26000000 INFO @ Sat, 03 Apr 2021 10:11:50: 20000000 INFO @ Sat, 03 Apr 2021 10:11:50: 31000000 INFO @ Sat, 03 Apr 2021 10:11:56: 27000000 INFO @ Sat, 03 Apr 2021 10:11:57: 32000000 INFO @ Sat, 03 Apr 2021 10:11:59: 21000000 INFO @ Sat, 03 Apr 2021 10:12:04: 33000000 INFO @ Sat, 03 Apr 2021 10:12:05: 28000000 INFO @ Sat, 03 Apr 2021 10:12:08: 22000000 INFO @ Sat, 03 Apr 2021 10:12:10: 34000000 INFO @ Sat, 03 Apr 2021 10:12:13: 29000000 INFO @ Sat, 03 Apr 2021 10:12:17: 23000000 INFO @ Sat, 03 Apr 2021 10:12:17: 35000000 INFO @ Sat, 03 Apr 2021 10:12:22: 30000000 INFO @ Sat, 03 Apr 2021 10:12:23: 36000000 INFO @ Sat, 03 Apr 2021 10:12:25: 24000000 INFO @ Sat, 03 Apr 2021 10:12:30: 31000000 INFO @ Sat, 03 Apr 2021 10:12:30: 37000000 INFO @ Sat, 03 Apr 2021 10:12:34: 25000000 INFO @ Sat, 03 Apr 2021 10:12:37: 38000000 INFO @ Sat, 03 Apr 2021 10:12:38: 32000000 INFO @ Sat, 03 Apr 2021 10:12:43: 26000000 INFO @ Sat, 03 Apr 2021 10:12:44: 39000000 INFO @ Sat, 03 Apr 2021 10:12:46: 33000000 INFO @ Sat, 03 Apr 2021 10:12:51: 40000000 INFO @ Sat, 03 Apr 2021 10:12:52: 27000000 INFO @ Sat, 03 Apr 2021 10:12:55: 34000000 INFO @ Sat, 03 Apr 2021 10:12:57: 41000000 INFO @ Sat, 03 Apr 2021 10:13:01: 28000000 INFO @ Sat, 03 Apr 2021 10:13:03: 35000000 INFO @ Sat, 03 Apr 2021 10:13:04: 42000000 INFO @ Sat, 03 Apr 2021 10:13:10: 29000000 INFO @ Sat, 03 Apr 2021 10:13:10: 43000000 INFO @ Sat, 03 Apr 2021 10:13:12: 36000000 INFO @ Sat, 03 Apr 2021 10:13:17: 44000000 INFO @ Sat, 03 Apr 2021 10:13:18: 30000000 INFO @ Sat, 03 Apr 2021 10:13:20: 37000000 INFO @ Sat, 03 Apr 2021 10:13:23: 45000000 INFO @ Sat, 03 Apr 2021 10:13:27: 31000000 INFO @ Sat, 03 Apr 2021 10:13:29: 38000000 INFO @ Sat, 03 Apr 2021 10:13:30: 46000000 INFO @ Sat, 03 Apr 2021 10:13:35: 32000000 INFO @ Sat, 03 Apr 2021 10:13:37: 47000000 INFO @ Sat, 03 Apr 2021 10:13:38: 39000000 INFO @ Sat, 03 Apr 2021 10:13:44: 48000000 INFO @ Sat, 03 Apr 2021 10:13:45: 33000000 INFO @ Sat, 03 Apr 2021 10:13:47: 40000000 INFO @ Sat, 03 Apr 2021 10:13:52: 49000000 INFO @ Sat, 03 Apr 2021 10:13:54: 34000000 INFO @ Sat, 03 Apr 2021 10:13:56: 41000000 INFO @ Sat, 03 Apr 2021 10:14:00: 50000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 10:14:03: 35000000 INFO @ Sat, 03 Apr 2021 10:14:05: 42000000 INFO @ Sat, 03 Apr 2021 10:14:08: 51000000 INFO @ Sat, 03 Apr 2021 10:14:12: 36000000 INFO @ Sat, 03 Apr 2021 10:14:15: 43000000 INFO @ Sat, 03 Apr 2021 10:14:15: 52000000 INFO @ Sat, 03 Apr 2021 10:14:21: 37000000 INFO @ Sat, 03 Apr 2021 10:14:23: 44000000 INFO @ Sat, 03 Apr 2021 10:14:23: 53000000 INFO @ Sat, 03 Apr 2021 10:14:30: 38000000 INFO @ Sat, 03 Apr 2021 10:14:31: 54000000 INFO @ Sat, 03 Apr 2021 10:14:31: 45000000 INFO @ Sat, 03 Apr 2021 10:14:39: 55000000 INFO @ Sat, 03 Apr 2021 10:14:39: 39000000 INFO @ Sat, 03 Apr 2021 10:14:40: 46000000 INFO @ Sat, 03 Apr 2021 10:14:48: 56000000 INFO @ Sat, 03 Apr 2021 10:14:48: 40000000 INFO @ Sat, 03 Apr 2021 10:14:49: 47000000 INFO @ Sat, 03 Apr 2021 10:14:56: 57000000 INFO @ Sat, 03 Apr 2021 10:14:57: 48000000 INFO @ Sat, 03 Apr 2021 10:14:57: 41000000 INFO @ Sat, 03 Apr 2021 10:15:05: 58000000 INFO @ Sat, 03 Apr 2021 10:15:06: 49000000 INFO @ Sat, 03 Apr 2021 10:15:07: 42000000 INFO @ Sat, 03 Apr 2021 10:15:13: 59000000 INFO @ Sat, 03 Apr 2021 10:15:16: 50000000 INFO @ Sat, 03 Apr 2021 10:15:17: 43000000 INFO @ Sat, 03 Apr 2021 10:15:22: 60000000 INFO @ Sat, 03 Apr 2021 10:15:25: 51000000 INFO @ Sat, 03 Apr 2021 10:15:26: 44000000 INFO @ Sat, 03 Apr 2021 10:15:32: 61000000 INFO @ Sat, 03 Apr 2021 10:15:34: 52000000 INFO @ Sat, 03 Apr 2021 10:15:35: 45000000 INFO @ Sat, 03 Apr 2021 10:15:41: 62000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 10:15:43: 53000000 INFO @ Sat, 03 Apr 2021 10:15:45: 46000000 INFO @ Sat, 03 Apr 2021 10:15:50: 63000000 INFO @ Sat, 03 Apr 2021 10:15:52: 54000000 INFO @ Sat, 03 Apr 2021 10:15:54: 47000000 INFO @ Sat, 03 Apr 2021 10:15:59: 64000000 INFO @ Sat, 03 Apr 2021 10:16:02: 55000000 INFO @ Sat, 03 Apr 2021 10:16:03: 48000000 INFO @ Sat, 03 Apr 2021 10:16:07: 65000000 INFO @ Sat, 03 Apr 2021 10:16:12: 56000000 INFO @ Sat, 03 Apr 2021 10:16:12: 49000000 INFO @ Sat, 03 Apr 2021 10:16:16: 66000000 INFO @ Sat, 03 Apr 2021 10:16:21: 57000000 INFO @ Sat, 03 Apr 2021 10:16:21: 50000000 INFO @ Sat, 03 Apr 2021 10:16:25: 67000000 INFO @ Sat, 03 Apr 2021 10:16:30: 58000000 INFO @ Sat, 03 Apr 2021 10:16:30: 51000000 INFO @ Sat, 03 Apr 2021 10:16:34: 68000000 INFO @ Sat, 03 Apr 2021 10:16:38: 52000000 INFO @ Sat, 03 Apr 2021 10:16:38: 59000000 INFO @ Sat, 03 Apr 2021 10:16:43: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 10:16:43: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 10:16:43: #1 total tags in treatment: 24936766 INFO @ Sat, 03 Apr 2021 10:16:43: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:16:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:16:43: #1 tags after filtering in treatment: 17498431 INFO @ Sat, 03 Apr 2021 10:16:43: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 03 Apr 2021 10:16:43: #1 finished! INFO @ Sat, 03 Apr 2021 10:16:43: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:16:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:16:44: #2 number of paired peaks: 814 WARNING @ Sat, 03 Apr 2021 10:16:44: Fewer paired peaks (814) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 814 pairs to build model! INFO @ Sat, 03 Apr 2021 10:16:44: start model_add_line... INFO @ Sat, 03 Apr 2021 10:16:45: start X-correlation... INFO @ Sat, 03 Apr 2021 10:16:45: end of X-cor INFO @ Sat, 03 Apr 2021 10:16:45: #2 finished! INFO @ Sat, 03 Apr 2021 10:16:45: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 10:16:45: #2 alternative fragment length(s) may be 4,100 bps INFO @ Sat, 03 Apr 2021 10:16:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.10_model.r WARNING @ Sat, 03 Apr 2021 10:16:45: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:16:45: #2 You may need to consider one of the other alternative d(s): 4,100 WARNING @ Sat, 03 Apr 2021 10:16:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:16:45: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:16:45: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:16:46: 53000000 INFO @ Sat, 03 Apr 2021 10:16:47: 60000000 INFO @ Sat, 03 Apr 2021 10:16:54: 54000000 INFO @ Sat, 03 Apr 2021 10:16:55: 61000000 INFO @ Sat, 03 Apr 2021 10:17:03: 55000000 INFO @ Sat, 03 Apr 2021 10:17:04: 62000000 INFO @ Sat, 03 Apr 2021 10:17:12: 56000000 INFO @ Sat, 03 Apr 2021 10:17:12: 63000000 INFO @ Sat, 03 Apr 2021 10:17:19: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:17:20: 57000000 INFO @ Sat, 03 Apr 2021 10:17:21: 64000000 INFO @ Sat, 03 Apr 2021 10:17:29: 58000000 INFO @ Sat, 03 Apr 2021 10:17:30: 65000000 INFO @ Sat, 03 Apr 2021 10:17:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.10_peaks.xls INFO @ Sat, 03 Apr 2021 10:17:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:17:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.10_summits.bed INFO @ Sat, 03 Apr 2021 10:17:37: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (4226 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:17:38: 59000000 INFO @ Sat, 03 Apr 2021 10:17:38: 66000000 INFO @ Sat, 03 Apr 2021 10:17:46: 60000000 INFO @ Sat, 03 Apr 2021 10:17:47: 67000000 INFO @ Sat, 03 Apr 2021 10:17:55: 61000000 INFO @ Sat, 03 Apr 2021 10:17:56: 68000000 INFO @ Sat, 03 Apr 2021 10:18:04: 62000000 INFO @ Sat, 03 Apr 2021 10:18:04: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 10:18:04: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 10:18:04: #1 total tags in treatment: 24936766 INFO @ Sat, 03 Apr 2021 10:18:04: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:18:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:18:05: #1 tags after filtering in treatment: 17498431 INFO @ Sat, 03 Apr 2021 10:18:05: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 03 Apr 2021 10:18:05: #1 finished! INFO @ Sat, 03 Apr 2021 10:18:05: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:18:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:18:06: #2 number of paired peaks: 814 WARNING @ Sat, 03 Apr 2021 10:18:06: Fewer paired peaks (814) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 814 pairs to build model! INFO @ Sat, 03 Apr 2021 10:18:06: start model_add_line... INFO @ Sat, 03 Apr 2021 10:18:06: start X-correlation... INFO @ Sat, 03 Apr 2021 10:18:06: end of X-cor INFO @ Sat, 03 Apr 2021 10:18:06: #2 finished! INFO @ Sat, 03 Apr 2021 10:18:06: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 10:18:06: #2 alternative fragment length(s) may be 4,100 bps INFO @ Sat, 03 Apr 2021 10:18:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.05_model.r WARNING @ Sat, 03 Apr 2021 10:18:06: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:18:06: #2 You may need to consider one of the other alternative d(s): 4,100 WARNING @ Sat, 03 Apr 2021 10:18:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:18:06: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:18:06: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:18:12: 63000000 INFO @ Sat, 03 Apr 2021 10:18:20: 64000000 INFO @ Sat, 03 Apr 2021 10:18:28: 65000000 INFO @ Sat, 03 Apr 2021 10:18:36: 66000000 INFO @ Sat, 03 Apr 2021 10:18:40: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:18:44: 67000000 INFO @ Sat, 03 Apr 2021 10:18:53: 68000000 INFO @ Sat, 03 Apr 2021 10:18:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.05_peaks.xls INFO @ Sat, 03 Apr 2021 10:18:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:18:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.05_summits.bed INFO @ Sat, 03 Apr 2021 10:18:58: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7807 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 10:19:02: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 10:19:02: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 10:19:02: #1 total tags in treatment: 24936766 INFO @ Sat, 03 Apr 2021 10:19:02: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 10:19:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 10:19:03: #1 tags after filtering in treatment: 17498431 INFO @ Sat, 03 Apr 2021 10:19:03: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 03 Apr 2021 10:19:03: #1 finished! INFO @ Sat, 03 Apr 2021 10:19:03: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 10:19:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 10:19:04: #2 number of paired peaks: 814 WARNING @ Sat, 03 Apr 2021 10:19:04: Fewer paired peaks (814) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 814 pairs to build model! INFO @ Sat, 03 Apr 2021 10:19:04: start model_add_line... INFO @ Sat, 03 Apr 2021 10:19:04: start X-correlation... INFO @ Sat, 03 Apr 2021 10:19:04: end of X-cor INFO @ Sat, 03 Apr 2021 10:19:04: #2 finished! INFO @ Sat, 03 Apr 2021 10:19:04: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 10:19:04: #2 alternative fragment length(s) may be 4,100 bps INFO @ Sat, 03 Apr 2021 10:19:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.20_model.r WARNING @ Sat, 03 Apr 2021 10:19:04: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 10:19:04: #2 You may need to consider one of the other alternative d(s): 4,100 WARNING @ Sat, 03 Apr 2021 10:19:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 10:19:04: #3 Call peaks... INFO @ Sat, 03 Apr 2021 10:19:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 10:19:38: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 10:19:55: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.20_peaks.xls INFO @ Sat, 03 Apr 2021 10:19:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 10:19:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3141076/SRX3141076.20_summits.bed INFO @ Sat, 03 Apr 2021 10:19:55: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1895 records, 4 fields): 4 millis CompletedMACS2peakCalling