Job ID = 12264931 SRX = SRX3088381 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 19815 spots for SRR5928059/SRR5928059.sra Written 19815 spots for SRR5928059/SRR5928059.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265074 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 19815 reads; of these: 19815 (100.00%) were unpaired; of these: 12128 (61.21%) aligned 0 times 5862 (29.58%) aligned exactly 1 time 1825 (9.21%) aligned >1 times 38.79% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 2187 / 7687 = 0.2845 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:00:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:00:46: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:00:46: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:00:46: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:00:46: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:00:46: #1 total tags in treatment: 5500 INFO @ Sat, 03 Apr 2021 06:00:46: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:00:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:00:46: #1 tags after filtering in treatment: 5496 INFO @ Sat, 03 Apr 2021 06:00:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:00:46: #1 finished! INFO @ Sat, 03 Apr 2021 06:00:46: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:00:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:00:46: #2 number of paired peaks: 195 WARNING @ Sat, 03 Apr 2021 06:00:46: Fewer paired peaks (195) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 195 pairs to build model! INFO @ Sat, 03 Apr 2021 06:00:46: start model_add_line... INFO @ Sat, 03 Apr 2021 06:00:46: start X-correlation... INFO @ Sat, 03 Apr 2021 06:00:46: end of X-cor INFO @ Sat, 03 Apr 2021 06:00:46: #2 finished! INFO @ Sat, 03 Apr 2021 06:00:46: #2 predicted fragment length is 8 bps INFO @ Sat, 03 Apr 2021 06:00:46: #2 alternative fragment length(s) may be 8,34,49,81,124,149,172,220,255,270,286,333,478,485 bps INFO @ Sat, 03 Apr 2021 06:00:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.05_model.r WARNING @ Sat, 03 Apr 2021 06:00:46: #2 Since the d (8) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:00:46: #2 You may need to consider one of the other alternative d(s): 8,34,49,81,124,149,172,220,255,270,286,333,478,485 WARNING @ Sat, 03 Apr 2021 06:00:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:00:46: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:00:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:00:46: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:00:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:00:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:00:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.05_summits.bed INFO @ Sat, 03 Apr 2021 06:00:46: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:01:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:01:16: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:01:16: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:01:16: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:01:16: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:01:16: #1 total tags in treatment: 5500 INFO @ Sat, 03 Apr 2021 06:01:16: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:01:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:01:16: #1 tags after filtering in treatment: 5496 INFO @ Sat, 03 Apr 2021 06:01:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:01:16: #1 finished! INFO @ Sat, 03 Apr 2021 06:01:16: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:01:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:01:16: #2 number of paired peaks: 195 WARNING @ Sat, 03 Apr 2021 06:01:16: Fewer paired peaks (195) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 195 pairs to build model! INFO @ Sat, 03 Apr 2021 06:01:16: start model_add_line... INFO @ Sat, 03 Apr 2021 06:01:16: start X-correlation... INFO @ Sat, 03 Apr 2021 06:01:16: end of X-cor INFO @ Sat, 03 Apr 2021 06:01:16: #2 finished! INFO @ Sat, 03 Apr 2021 06:01:16: #2 predicted fragment length is 8 bps INFO @ Sat, 03 Apr 2021 06:01:16: #2 alternative fragment length(s) may be 8,34,49,81,124,149,172,220,255,270,286,333,478,485 bps INFO @ Sat, 03 Apr 2021 06:01:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.10_model.r WARNING @ Sat, 03 Apr 2021 06:01:16: #2 Since the d (8) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:01:16: #2 You may need to consider one of the other alternative d(s): 8,34,49,81,124,149,172,220,255,270,286,333,478,485 WARNING @ Sat, 03 Apr 2021 06:01:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:01:16: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:01:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:01:16: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:01:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:01:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:01:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.10_summits.bed INFO @ Sat, 03 Apr 2021 06:01:16: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:01:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:01:46: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:01:46: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:01:46: #1 tag size is determined as 74 bps INFO @ Sat, 03 Apr 2021 06:01:46: #1 tag size = 74 INFO @ Sat, 03 Apr 2021 06:01:46: #1 total tags in treatment: 5500 INFO @ Sat, 03 Apr 2021 06:01:46: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:01:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:01:46: #1 tags after filtering in treatment: 5496 INFO @ Sat, 03 Apr 2021 06:01:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:01:46: #1 finished! INFO @ Sat, 03 Apr 2021 06:01:46: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:01:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:01:46: #2 number of paired peaks: 195 WARNING @ Sat, 03 Apr 2021 06:01:46: Fewer paired peaks (195) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 195 pairs to build model! INFO @ Sat, 03 Apr 2021 06:01:46: start model_add_line... INFO @ Sat, 03 Apr 2021 06:01:46: start X-correlation... INFO @ Sat, 03 Apr 2021 06:01:46: end of X-cor INFO @ Sat, 03 Apr 2021 06:01:46: #2 finished! INFO @ Sat, 03 Apr 2021 06:01:46: #2 predicted fragment length is 8 bps INFO @ Sat, 03 Apr 2021 06:01:46: #2 alternative fragment length(s) may be 8,34,49,81,124,149,172,220,255,270,286,333,478,485 bps INFO @ Sat, 03 Apr 2021 06:01:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.20_model.r WARNING @ Sat, 03 Apr 2021 06:01:46: #2 Since the d (8) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 06:01:46: #2 You may need to consider one of the other alternative d(s): 8,34,49,81,124,149,172,220,255,270,286,333,478,485 WARNING @ Sat, 03 Apr 2021 06:01:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 06:01:46: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:01:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:01:46: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:01:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:01:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:01:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3088381/SRX3088381.20_summits.bed INFO @ Sat, 03 Apr 2021 06:01:46: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling