Job ID = 6527889 SRX = SRX3068983 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:42:11 prefetch.2.10.7: 1) Downloading 'SRR5907459'... 2020-06-29T13:42:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:45:16 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:45:16 prefetch.2.10.7: 1) 'SRR5907459' was downloaded successfully Read 18188488 spots for SRR5907459/SRR5907459.sra Written 18188488 spots for SRR5907459/SRR5907459.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:47 18188488 reads; of these: 18188488 (100.00%) were unpaired; of these: 1133574 (6.23%) aligned 0 times 13648805 (75.04%) aligned exactly 1 time 3406109 (18.73%) aligned >1 times 93.77% overall alignment rate Time searching: 00:05:48 Overall time: 00:05:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4898557 / 17054914 = 0.2872 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:04:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:04:04: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:04:04: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:04:13: 1000000 INFO @ Mon, 29 Jun 2020 23:04:21: 2000000 INFO @ Mon, 29 Jun 2020 23:04:30: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:04:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:04:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:04:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:04:39: 4000000 INFO @ Mon, 29 Jun 2020 23:04:44: 1000000 INFO @ Mon, 29 Jun 2020 23:04:47: 5000000 INFO @ Mon, 29 Jun 2020 23:04:52: 2000000 INFO @ Mon, 29 Jun 2020 23:04:56: 6000000 INFO @ Mon, 29 Jun 2020 23:05:01: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:05: 7000000 INFO @ Mon, 29 Jun 2020 23:05:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:11: 4000000 INFO @ Mon, 29 Jun 2020 23:05:13: 8000000 INFO @ Mon, 29 Jun 2020 23:05:15: 1000000 INFO @ Mon, 29 Jun 2020 23:05:20: 5000000 INFO @ Mon, 29 Jun 2020 23:05:22: 9000000 INFO @ Mon, 29 Jun 2020 23:05:24: 2000000 INFO @ Mon, 29 Jun 2020 23:05:28: 6000000 INFO @ Mon, 29 Jun 2020 23:05:31: 10000000 INFO @ Mon, 29 Jun 2020 23:05:32: 3000000 INFO @ Mon, 29 Jun 2020 23:05:37: 7000000 INFO @ Mon, 29 Jun 2020 23:05:39: 11000000 INFO @ Mon, 29 Jun 2020 23:05:42: 4000000 INFO @ Mon, 29 Jun 2020 23:05:46: 8000000 INFO @ Mon, 29 Jun 2020 23:05:48: 12000000 INFO @ Mon, 29 Jun 2020 23:05:50: 5000000 INFO @ Mon, 29 Jun 2020 23:05:50: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:05:50: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:05:50: #1 total tags in treatment: 12156357 INFO @ Mon, 29 Jun 2020 23:05:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:05:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:05:51: #1 tags after filtering in treatment: 12156357 INFO @ Mon, 29 Jun 2020 23:05:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:05:51: #1 finished! INFO @ Mon, 29 Jun 2020 23:05:51: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:05:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:05:51: #2 number of paired peaks: 97 WARNING @ Mon, 29 Jun 2020 23:05:51: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:05:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:05:54: 9000000 INFO @ Mon, 29 Jun 2020 23:05:57: 6000000 INFO @ Mon, 29 Jun 2020 23:06:01: 10000000 INFO @ Mon, 29 Jun 2020 23:06:04: 7000000 INFO @ Mon, 29 Jun 2020 23:06:08: 11000000 INFO @ Mon, 29 Jun 2020 23:06:10: 8000000 INFO @ Mon, 29 Jun 2020 23:06:14: 12000000 INFO @ Mon, 29 Jun 2020 23:06:15: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:06:15: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:06:15: #1 total tags in treatment: 12156357 INFO @ Mon, 29 Jun 2020 23:06:15: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:06:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:06:16: #1 tags after filtering in treatment: 12156357 INFO @ Mon, 29 Jun 2020 23:06:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:06:16: #1 finished! INFO @ Mon, 29 Jun 2020 23:06:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:06:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:06:17: #2 number of paired peaks: 97 WARNING @ Mon, 29 Jun 2020 23:06:17: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:06:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:06:17: 9000000 INFO @ Mon, 29 Jun 2020 23:06:24: 10000000 INFO @ Mon, 29 Jun 2020 23:06:31: 11000000 INFO @ Mon, 29 Jun 2020 23:06:37: 12000000 INFO @ Mon, 29 Jun 2020 23:06:38: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:06:38: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:06:38: #1 total tags in treatment: 12156357 INFO @ Mon, 29 Jun 2020 23:06:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:06:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:06:38: #1 tags after filtering in treatment: 12156357 INFO @ Mon, 29 Jun 2020 23:06:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:06:38: #1 finished! INFO @ Mon, 29 Jun 2020 23:06:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:06:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:06:39: #2 number of paired peaks: 97 WARNING @ Mon, 29 Jun 2020 23:06:39: Too few paired peaks (97) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:06:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068983/SRX3068983.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。