Job ID = 6527888 SRX = SRX3068968 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:43:44 prefetch.2.10.7: 1) Downloading 'SRR5907444'... 2020-06-29T13:43:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:49:10 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:49:10 prefetch.2.10.7: 1) 'SRR5907444' was downloaded successfully Read 21016576 spots for SRR5907444/SRR5907444.sra Written 21016576 spots for SRR5907444/SRR5907444.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:49 21016576 reads; of these: 21016576 (100.00%) were unpaired; of these: 1581171 (7.52%) aligned 0 times 16297704 (77.55%) aligned exactly 1 time 3137701 (14.93%) aligned >1 times 92.48% overall alignment rate Time searching: 00:05:49 Overall time: 00:05:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5019892 / 19435405 = 0.2583 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:33: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:33: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:40: 1000000 INFO @ Mon, 29 Jun 2020 23:05:47: 2000000 INFO @ Mon, 29 Jun 2020 23:05:53: 3000000 INFO @ Mon, 29 Jun 2020 23:06:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:03: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:03: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:07: 5000000 INFO @ Mon, 29 Jun 2020 23:06:11: 1000000 INFO @ Mon, 29 Jun 2020 23:06:14: 6000000 INFO @ Mon, 29 Jun 2020 23:06:18: 2000000 INFO @ Mon, 29 Jun 2020 23:06:21: 7000000 INFO @ Mon, 29 Jun 2020 23:06:25: 3000000 INFO @ Mon, 29 Jun 2020 23:06:28: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:33: 4000000 INFO @ Mon, 29 Jun 2020 23:06:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:33: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:33: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:35: 9000000 INFO @ Mon, 29 Jun 2020 23:06:40: 5000000 INFO @ Mon, 29 Jun 2020 23:06:40: 1000000 INFO @ Mon, 29 Jun 2020 23:06:43: 10000000 INFO @ Mon, 29 Jun 2020 23:06:47: 2000000 INFO @ Mon, 29 Jun 2020 23:06:47: 6000000 INFO @ Mon, 29 Jun 2020 23:06:50: 11000000 INFO @ Mon, 29 Jun 2020 23:06:54: 3000000 INFO @ Mon, 29 Jun 2020 23:06:55: 7000000 INFO @ Mon, 29 Jun 2020 23:06:57: 12000000 INFO @ Mon, 29 Jun 2020 23:07:01: 4000000 INFO @ Mon, 29 Jun 2020 23:07:03: 8000000 INFO @ Mon, 29 Jun 2020 23:07:05: 13000000 INFO @ Mon, 29 Jun 2020 23:07:08: 5000000 INFO @ Mon, 29 Jun 2020 23:07:10: 9000000 INFO @ Mon, 29 Jun 2020 23:07:12: 14000000 INFO @ Mon, 29 Jun 2020 23:07:14: 6000000 INFO @ Mon, 29 Jun 2020 23:07:15: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:07:15: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:07:15: #1 total tags in treatment: 14415513 INFO @ Mon, 29 Jun 2020 23:07:15: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:15: #1 tags after filtering in treatment: 14415513 INFO @ Mon, 29 Jun 2020 23:07:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:15: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:15: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:16: #2 number of paired peaks: 79 WARNING @ Mon, 29 Jun 2020 23:07:16: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:07:18: 10000000 INFO @ Mon, 29 Jun 2020 23:07:21: 7000000 INFO @ Mon, 29 Jun 2020 23:07:25: 11000000 INFO @ Mon, 29 Jun 2020 23:07:28: 8000000 INFO @ Mon, 29 Jun 2020 23:07:32: 12000000 INFO @ Mon, 29 Jun 2020 23:07:35: 9000000 INFO @ Mon, 29 Jun 2020 23:07:39: 13000000 INFO @ Mon, 29 Jun 2020 23:07:41: 10000000 INFO @ Mon, 29 Jun 2020 23:07:47: 14000000 INFO @ Mon, 29 Jun 2020 23:07:48: 11000000 INFO @ Mon, 29 Jun 2020 23:07:50: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:07:50: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:07:50: #1 total tags in treatment: 14415513 INFO @ Mon, 29 Jun 2020 23:07:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:50: #1 tags after filtering in treatment: 14415513 INFO @ Mon, 29 Jun 2020 23:07:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:50: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:51: #2 number of paired peaks: 79 WARNING @ Mon, 29 Jun 2020 23:07:51: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:07:54: 12000000 INFO @ Mon, 29 Jun 2020 23:08:01: 13000000 INFO @ Mon, 29 Jun 2020 23:08:07: 14000000 INFO @ Mon, 29 Jun 2020 23:08:09: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:08:09: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:08:09: #1 total tags in treatment: 14415513 INFO @ Mon, 29 Jun 2020 23:08:09: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:09: #1 tags after filtering in treatment: 14415513 INFO @ Mon, 29 Jun 2020 23:08:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:09: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:09: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:10: #2 number of paired peaks: 79 WARNING @ Mon, 29 Jun 2020 23:08:10: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068968/SRX3068968.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。