Job ID = 6527887 SRX = SRX3068967 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:59:48 prefetch.2.10.7: 1) Downloading 'SRR5907443'... 2020-06-29T13:59:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:02:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:02:58 prefetch.2.10.7: 1) 'SRR5907443' was downloaded successfully Read 21069300 spots for SRR5907443/SRR5907443.sra Written 21069300 spots for SRR5907443/SRR5907443.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:39 21069300 reads; of these: 21069300 (100.00%) were unpaired; of these: 990442 (4.70%) aligned 0 times 15916805 (75.55%) aligned exactly 1 time 4162053 (19.75%) aligned >1 times 95.30% overall alignment rate Time searching: 00:06:39 Overall time: 00:06:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2599727 / 20078858 = 0.1295 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:20:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:20:20: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:20:20: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:20:26: 1000000 INFO @ Mon, 29 Jun 2020 23:20:31: 2000000 INFO @ Mon, 29 Jun 2020 23:20:36: 3000000 INFO @ Mon, 29 Jun 2020 23:20:41: 4000000 INFO @ Mon, 29 Jun 2020 23:20:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:20:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:20:50: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:20:50: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:20:52: 6000000 INFO @ Mon, 29 Jun 2020 23:20:57: 1000000 INFO @ Mon, 29 Jun 2020 23:20:58: 7000000 INFO @ Mon, 29 Jun 2020 23:21:03: 2000000 INFO @ Mon, 29 Jun 2020 23:21:03: 8000000 INFO @ Mon, 29 Jun 2020 23:21:09: 9000000 INFO @ Mon, 29 Jun 2020 23:21:09: 3000000 INFO @ Mon, 29 Jun 2020 23:21:15: 10000000 INFO @ Mon, 29 Jun 2020 23:21:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:21:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:21:20: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:21:20: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:21:21: 11000000 INFO @ Mon, 29 Jun 2020 23:21:22: 5000000 INFO @ Mon, 29 Jun 2020 23:21:26: 1000000 INFO @ Mon, 29 Jun 2020 23:21:27: 12000000 INFO @ Mon, 29 Jun 2020 23:21:28: 6000000 INFO @ Mon, 29 Jun 2020 23:21:32: 2000000 INFO @ Mon, 29 Jun 2020 23:21:33: 13000000 INFO @ Mon, 29 Jun 2020 23:21:35: 7000000 INFO @ Mon, 29 Jun 2020 23:21:37: 3000000 INFO @ Mon, 29 Jun 2020 23:21:38: 14000000 INFO @ Mon, 29 Jun 2020 23:21:41: 8000000 INFO @ Mon, 29 Jun 2020 23:21:43: 4000000 INFO @ Mon, 29 Jun 2020 23:21:44: 15000000 INFO @ Mon, 29 Jun 2020 23:21:47: 9000000 INFO @ Mon, 29 Jun 2020 23:21:49: 5000000 INFO @ Mon, 29 Jun 2020 23:21:50: 16000000 INFO @ Mon, 29 Jun 2020 23:21:54: 10000000 INFO @ Mon, 29 Jun 2020 23:21:54: 6000000 INFO @ Mon, 29 Jun 2020 23:21:56: 17000000 INFO @ Mon, 29 Jun 2020 23:21:58: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:21:58: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:21:58: #1 total tags in treatment: 17479131 INFO @ Mon, 29 Jun 2020 23:21:58: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:21:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:21:59: #1 tags after filtering in treatment: 17479131 INFO @ Mon, 29 Jun 2020 23:21:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:21:59: #1 finished! INFO @ Mon, 29 Jun 2020 23:21:59: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:21:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:22:00: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:22:00: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:22:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:22:00: 11000000 INFO @ Mon, 29 Jun 2020 23:22:00: 7000000 INFO @ Mon, 29 Jun 2020 23:22:06: 8000000 INFO @ Mon, 29 Jun 2020 23:22:06: 12000000 INFO @ Mon, 29 Jun 2020 23:22:11: 9000000 INFO @ Mon, 29 Jun 2020 23:22:12: 13000000 INFO @ Mon, 29 Jun 2020 23:22:17: 10000000 INFO @ Mon, 29 Jun 2020 23:22:19: 14000000 INFO @ Mon, 29 Jun 2020 23:22:23: 11000000 INFO @ Mon, 29 Jun 2020 23:22:25: 15000000 INFO @ Mon, 29 Jun 2020 23:22:28: 12000000 INFO @ Mon, 29 Jun 2020 23:22:31: 16000000 INFO @ Mon, 29 Jun 2020 23:22:34: 13000000 INFO @ Mon, 29 Jun 2020 23:22:37: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:22:40: 14000000 INFO @ Mon, 29 Jun 2020 23:22:40: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:22:40: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:22:40: #1 total tags in treatment: 17479131 INFO @ Mon, 29 Jun 2020 23:22:40: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:22:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:22:41: #1 tags after filtering in treatment: 17479131 INFO @ Mon, 29 Jun 2020 23:22:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:22:41: #1 finished! INFO @ Mon, 29 Jun 2020 23:22:41: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:22:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:22:42: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:22:42: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:22:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:22:45: 15000000 INFO @ Mon, 29 Jun 2020 23:22:50: 16000000 INFO @ Mon, 29 Jun 2020 23:22:56: 17000000 INFO @ Mon, 29 Jun 2020 23:22:59: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:22:59: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:22:59: #1 total tags in treatment: 17479131 INFO @ Mon, 29 Jun 2020 23:22:59: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:22:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:22:59: #1 tags after filtering in treatment: 17479131 INFO @ Mon, 29 Jun 2020 23:22:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:22:59: #1 finished! INFO @ Mon, 29 Jun 2020 23:22:59: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:22:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:23:00: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:23:00: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:23:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3068967/SRX3068967.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。