Job ID = 12264854 SRX = SRX3032253 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 17477777 spots for SRR5863943/SRR5863943.sra Written 17477777 spots for SRR5863943/SRR5863943.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265040 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:11 17477777 reads; of these: 17477777 (100.00%) were unpaired; of these: 7037543 (40.27%) aligned 0 times 6112423 (34.97%) aligned exactly 1 time 4327811 (24.76%) aligned >1 times 59.73% overall alignment rate Time searching: 00:03:11 Overall time: 00:03:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 4719710 / 10440234 = 0.4521 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 05:59:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 05:59:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 05:59:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:00:01: 1000000 INFO @ Sat, 03 Apr 2021 06:00:07: 2000000 INFO @ Sat, 03 Apr 2021 06:00:14: 3000000 INFO @ Sat, 03 Apr 2021 06:00:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:00:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:00:24: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:00:24: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:00:27: 5000000 INFO @ Sat, 03 Apr 2021 06:00:30: 1000000 INFO @ Sat, 03 Apr 2021 06:00:32: #1 tag size is determined as 20 bps INFO @ Sat, 03 Apr 2021 06:00:32: #1 tag size = 20 INFO @ Sat, 03 Apr 2021 06:00:32: #1 total tags in treatment: 5720524 INFO @ Sat, 03 Apr 2021 06:00:32: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:00:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:00:32: #1 tags after filtering in treatment: 5720524 INFO @ Sat, 03 Apr 2021 06:00:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:00:32: #1 finished! INFO @ Sat, 03 Apr 2021 06:00:32: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:00:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:00:33: #2 number of paired peaks: 1169 INFO @ Sat, 03 Apr 2021 06:00:33: start model_add_line... INFO @ Sat, 03 Apr 2021 06:00:33: start X-correlation... INFO @ Sat, 03 Apr 2021 06:00:33: end of X-cor INFO @ Sat, 03 Apr 2021 06:00:33: #2 finished! INFO @ Sat, 03 Apr 2021 06:00:33: #2 predicted fragment length is 63 bps INFO @ Sat, 03 Apr 2021 06:00:33: #2 alternative fragment length(s) may be 63 bps INFO @ Sat, 03 Apr 2021 06:00:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.05_model.r INFO @ Sat, 03 Apr 2021 06:00:33: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:00:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:00:37: 2000000 INFO @ Sat, 03 Apr 2021 06:00:43: 3000000 INFO @ Sat, 03 Apr 2021 06:00:50: 4000000 INFO @ Sat, 03 Apr 2021 06:00:50: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 06:00:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 06:00:54: #1 read tag files... INFO @ Sat, 03 Apr 2021 06:00:54: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 06:00:56: 5000000 INFO @ Sat, 03 Apr 2021 06:01:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.05_peaks.xls INFO @ Sat, 03 Apr 2021 06:01:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:01:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.05_summits.bed INFO @ Sat, 03 Apr 2021 06:01:00: Done! pass1 - making usageList (14 chroms): 6 millis pass2 - checking and writing primary data (9655 records, 4 fields): 14 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 06:01:01: 1000000 INFO @ Sat, 03 Apr 2021 06:01:01: #1 tag size is determined as 20 bps INFO @ Sat, 03 Apr 2021 06:01:01: #1 tag size = 20 INFO @ Sat, 03 Apr 2021 06:01:01: #1 total tags in treatment: 5720524 INFO @ Sat, 03 Apr 2021 06:01:01: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:01:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:01:01: #1 tags after filtering in treatment: 5720524 INFO @ Sat, 03 Apr 2021 06:01:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:01:01: #1 finished! INFO @ Sat, 03 Apr 2021 06:01:01: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:01:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:01:02: #2 number of paired peaks: 1169 INFO @ Sat, 03 Apr 2021 06:01:02: start model_add_line... INFO @ Sat, 03 Apr 2021 06:01:02: start X-correlation... INFO @ Sat, 03 Apr 2021 06:01:02: end of X-cor INFO @ Sat, 03 Apr 2021 06:01:02: #2 finished! INFO @ Sat, 03 Apr 2021 06:01:02: #2 predicted fragment length is 63 bps INFO @ Sat, 03 Apr 2021 06:01:02: #2 alternative fragment length(s) may be 63 bps INFO @ Sat, 03 Apr 2021 06:01:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.10_model.r INFO @ Sat, 03 Apr 2021 06:01:02: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:01:02: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 06:01:07: 2000000 INFO @ Sat, 03 Apr 2021 06:01:13: 3000000 INFO @ Sat, 03 Apr 2021 06:01:19: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:01:20: 4000000 INFO @ Sat, 03 Apr 2021 06:01:26: 5000000 INFO @ Sat, 03 Apr 2021 06:01:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.10_peaks.xls INFO @ Sat, 03 Apr 2021 06:01:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:01:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.10_summits.bed INFO @ Sat, 03 Apr 2021 06:01:29: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (3884 records, 4 fields): 7 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 06:01:31: #1 tag size is determined as 20 bps INFO @ Sat, 03 Apr 2021 06:01:31: #1 tag size = 20 INFO @ Sat, 03 Apr 2021 06:01:31: #1 total tags in treatment: 5720524 INFO @ Sat, 03 Apr 2021 06:01:31: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 06:01:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 06:01:31: #1 tags after filtering in treatment: 5720524 INFO @ Sat, 03 Apr 2021 06:01:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Apr 2021 06:01:31: #1 finished! INFO @ Sat, 03 Apr 2021 06:01:31: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 06:01:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 06:01:32: #2 number of paired peaks: 1169 INFO @ Sat, 03 Apr 2021 06:01:32: start model_add_line... INFO @ Sat, 03 Apr 2021 06:01:32: start X-correlation... INFO @ Sat, 03 Apr 2021 06:01:32: end of X-cor INFO @ Sat, 03 Apr 2021 06:01:32: #2 finished! INFO @ Sat, 03 Apr 2021 06:01:32: #2 predicted fragment length is 63 bps INFO @ Sat, 03 Apr 2021 06:01:32: #2 alternative fragment length(s) may be 63 bps INFO @ Sat, 03 Apr 2021 06:01:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.20_model.r INFO @ Sat, 03 Apr 2021 06:01:32: #3 Call peaks... INFO @ Sat, 03 Apr 2021 06:01:32: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 06:01:49: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 06:01:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.20_peaks.xls INFO @ Sat, 03 Apr 2021 06:01:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 06:01:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3032253/SRX3032253.20_summits.bed INFO @ Sat, 03 Apr 2021 06:01:58: Done! pass1 - making usageList (9 chroms): 2 millis pass2 - checking and writing primary data (410 records, 4 fields): 4 millis CompletedMACS2peakCalling