Job ID = 10609115 sra ファイルのダウンロード中... Completed: 1019426K bytes transferred in 106 seconds (78460K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 41350758 spots for /home/okishinya/chipatlas/results/dm3/SRX3011253/SRR5834730.sra Written 41350758 spots for /home/okishinya/chipatlas/results/dm3/SRX3011253/SRR5834730.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:07 41350758 reads; of these: 41350758 (100.00%) were unpaired; of these: 1205228 (2.91%) aligned 0 times 38194852 (92.37%) aligned exactly 1 time 1950678 (4.72%) aligned >1 times 97.09% overall alignment rate Time searching: 00:08:07 Overall time: 00:08:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 18369081 / 40145530 = 0.4576 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 04 May 2018 07:20:44: # Command line: callpeak -t SRX3011253.bam -f BAM -g dm -n SRX3011253.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3011253.05 # format = BAM # ChIP-seq file = ['SRX3011253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:20:44: #1 read tag files... INFO @ Fri, 04 May 2018 07:20:44: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:20:44: # Command line: callpeak -t SRX3011253.bam -f BAM -g dm -n SRX3011253.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3011253.20 # format = BAM # ChIP-seq file = ['SRX3011253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:20:44: #1 read tag files... INFO @ Fri, 04 May 2018 07:20:44: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:20:44: # Command line: callpeak -t SRX3011253.bam -f BAM -g dm -n SRX3011253.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3011253.10 # format = BAM # ChIP-seq file = ['SRX3011253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:20:44: #1 read tag files... INFO @ Fri, 04 May 2018 07:20:44: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:20:50: 1000000 INFO @ Fri, 04 May 2018 07:20:50: 1000000 INFO @ Fri, 04 May 2018 07:20:50: 1000000 INFO @ Fri, 04 May 2018 07:20:56: 2000000 INFO @ Fri, 04 May 2018 07:20:57: 2000000 INFO @ Fri, 04 May 2018 07:20:57: 2000000 INFO @ Fri, 04 May 2018 07:21:03: 3000000 INFO @ Fri, 04 May 2018 07:21:03: 3000000 INFO @ Fri, 04 May 2018 07:21:03: 3000000 INFO @ Fri, 04 May 2018 07:21:09: 4000000 INFO @ Fri, 04 May 2018 07:21:09: 4000000 INFO @ Fri, 04 May 2018 07:21:10: 4000000 INFO @ Fri, 04 May 2018 07:21:16: 5000000 INFO @ Fri, 04 May 2018 07:21:16: 5000000 INFO @ Fri, 04 May 2018 07:21:16: 5000000 INFO @ Fri, 04 May 2018 07:21:22: 6000000 INFO @ Fri, 04 May 2018 07:21:22: 6000000 INFO @ Fri, 04 May 2018 07:21:23: 6000000 INFO @ Fri, 04 May 2018 07:21:28: 7000000 INFO @ Fri, 04 May 2018 07:21:29: 7000000 INFO @ Fri, 04 May 2018 07:21:29: 7000000 INFO @ Fri, 04 May 2018 07:21:35: 8000000 INFO @ Fri, 04 May 2018 07:21:35: 8000000 INFO @ Fri, 04 May 2018 07:21:36: 8000000 INFO @ Fri, 04 May 2018 07:21:41: 9000000 INFO @ Fri, 04 May 2018 07:21:42: 9000000 INFO @ Fri, 04 May 2018 07:21:43: 9000000 INFO @ Fri, 04 May 2018 07:21:47: 10000000 INFO @ Fri, 04 May 2018 07:21:48: 10000000 INFO @ Fri, 04 May 2018 07:21:50: 10000000 INFO @ Fri, 04 May 2018 07:21:53: 11000000 INFO @ Fri, 04 May 2018 07:21:55: 11000000 INFO @ Fri, 04 May 2018 07:21:56: 11000000 INFO @ Fri, 04 May 2018 07:22:00: 12000000 INFO @ Fri, 04 May 2018 07:22:01: 12000000 INFO @ Fri, 04 May 2018 07:22:03: 12000000 INFO @ Fri, 04 May 2018 07:22:06: 13000000 INFO @ Fri, 04 May 2018 07:22:08: 13000000 INFO @ Fri, 04 May 2018 07:22:09: 13000000 INFO @ Fri, 04 May 2018 07:22:12: 14000000 INFO @ Fri, 04 May 2018 07:22:14: 14000000 INFO @ Fri, 04 May 2018 07:22:16: 14000000 INFO @ Fri, 04 May 2018 07:22:18: 15000000 INFO @ Fri, 04 May 2018 07:22:20: 15000000 INFO @ Fri, 04 May 2018 07:22:22: 15000000 INFO @ Fri, 04 May 2018 07:22:25: 16000000 INFO @ Fri, 04 May 2018 07:22:27: 16000000 INFO @ Fri, 04 May 2018 07:22:29: 16000000 INFO @ Fri, 04 May 2018 07:22:31: 17000000 INFO @ Fri, 04 May 2018 07:22:33: 17000000 INFO @ Fri, 04 May 2018 07:22:36: 17000000 INFO @ Fri, 04 May 2018 07:22:37: 18000000 INFO @ Fri, 04 May 2018 07:22:39: 18000000 INFO @ Fri, 04 May 2018 07:22:42: 18000000 INFO @ Fri, 04 May 2018 07:22:43: 19000000 INFO @ Fri, 04 May 2018 07:22:46: 19000000 INFO @ Fri, 04 May 2018 07:22:49: 19000000 INFO @ Fri, 04 May 2018 07:22:50: 20000000 INFO @ Fri, 04 May 2018 07:22:53: 20000000 INFO @ Fri, 04 May 2018 07:22:56: 21000000 INFO @ Fri, 04 May 2018 07:22:56: 20000000 INFO @ Fri, 04 May 2018 07:22:59: 21000000 INFO @ Fri, 04 May 2018 07:23:01: #1 tag size is determined as 50 bps INFO @ Fri, 04 May 2018 07:23:01: #1 tag size = 50 INFO @ Fri, 04 May 2018 07:23:01: #1 total tags in treatment: 21776449 INFO @ Fri, 04 May 2018 07:23:01: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:23:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:23:01: #1 tags after filtering in treatment: 21776449 INFO @ Fri, 04 May 2018 07:23:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:23:01: #1 finished! INFO @ Fri, 04 May 2018 07:23:01: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:23:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:23:03: #2 number of paired peaks: 9 WARNING @ Fri, 04 May 2018 07:23:03: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:23:03: Process for pairing-model is terminated! cat: SRX3011253.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3011253.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011253.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011253.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 04 May 2018 07:23:03: 21000000 INFO @ Fri, 04 May 2018 07:23:05: #1 tag size is determined as 50 bps INFO @ Fri, 04 May 2018 07:23:05: #1 tag size = 50 INFO @ Fri, 04 May 2018 07:23:05: #1 total tags in treatment: 21776449 INFO @ Fri, 04 May 2018 07:23:05: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:23:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:23:05: #1 tags after filtering in treatment: 21776449 INFO @ Fri, 04 May 2018 07:23:05: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:23:05: #1 finished! INFO @ Fri, 04 May 2018 07:23:05: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:23:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:23:06: #2 number of paired peaks: 9 WARNING @ Fri, 04 May 2018 07:23:06: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:23:06: Process for pairing-model is terminated! cat: SRX3011253.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3011253.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011253.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011253.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 04 May 2018 07:23:08: #1 tag size is determined as 50 bps INFO @ Fri, 04 May 2018 07:23:08: #1 tag size = 50 INFO @ Fri, 04 May 2018 07:23:08: #1 total tags in treatment: 21776449 INFO @ Fri, 04 May 2018 07:23:08: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:23:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:23:09: #1 tags after filtering in treatment: 21776449 INFO @ Fri, 04 May 2018 07:23:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:23:09: #1 finished! INFO @ Fri, 04 May 2018 07:23:09: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:23:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:23:10: #2 number of paired peaks: 9 WARNING @ Fri, 04 May 2018 07:23:10: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:23:10: Process for pairing-model is terminated! cat: SRX3011253.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3011253.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011253.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011253.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。