Job ID = 10609088 sra ファイルのダウンロード中... Completed: 677906K bytes transferred in 51 seconds (108207K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 29094987 spots for /home/okishinya/chipatlas/results/dm3/SRX3011226/SRR5834703.sra Written 29094987 spots for /home/okishinya/chipatlas/results/dm3/SRX3011226/SRR5834703.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:35 29094987 reads; of these: 29094987 (100.00%) were unpaired; of these: 426824 (1.47%) aligned 0 times 27601056 (94.87%) aligned exactly 1 time 1067107 (3.67%) aligned >1 times 98.53% overall alignment rate Time searching: 00:05:36 Overall time: 00:05:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9680494 / 28668163 = 0.3377 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 04 May 2018 07:15:45: # Command line: callpeak -t SRX3011226.bam -f BAM -g dm -n SRX3011226.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3011226.05 # format = BAM # ChIP-seq file = ['SRX3011226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:15:45: #1 read tag files... INFO @ Fri, 04 May 2018 07:15:45: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:15:45: # Command line: callpeak -t SRX3011226.bam -f BAM -g dm -n SRX3011226.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3011226.10 # format = BAM # ChIP-seq file = ['SRX3011226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:15:45: #1 read tag files... INFO @ Fri, 04 May 2018 07:15:45: # Command line: callpeak -t SRX3011226.bam -f BAM -g dm -n SRX3011226.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3011226.20 # format = BAM # ChIP-seq file = ['SRX3011226.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:15:45: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:15:45: #1 read tag files... INFO @ Fri, 04 May 2018 07:15:45: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:15:52: 1000000 INFO @ Fri, 04 May 2018 07:15:52: 1000000 INFO @ Fri, 04 May 2018 07:15:52: 1000000 INFO @ Fri, 04 May 2018 07:15:59: 2000000 INFO @ Fri, 04 May 2018 07:15:59: 2000000 INFO @ Fri, 04 May 2018 07:15:59: 2000000 INFO @ Fri, 04 May 2018 07:16:06: 3000000 INFO @ Fri, 04 May 2018 07:16:06: 3000000 INFO @ Fri, 04 May 2018 07:16:06: 3000000 INFO @ Fri, 04 May 2018 07:16:13: 4000000 INFO @ Fri, 04 May 2018 07:16:13: 4000000 INFO @ Fri, 04 May 2018 07:16:14: 4000000 INFO @ Fri, 04 May 2018 07:16:20: 5000000 INFO @ Fri, 04 May 2018 07:16:20: 5000000 INFO @ Fri, 04 May 2018 07:16:21: 5000000 INFO @ Fri, 04 May 2018 07:16:26: 6000000 INFO @ Fri, 04 May 2018 07:16:27: 6000000 INFO @ Fri, 04 May 2018 07:16:28: 6000000 INFO @ Fri, 04 May 2018 07:16:33: 7000000 INFO @ Fri, 04 May 2018 07:16:34: 7000000 INFO @ Fri, 04 May 2018 07:16:35: 7000000 INFO @ Fri, 04 May 2018 07:16:40: 8000000 INFO @ Fri, 04 May 2018 07:16:41: 8000000 INFO @ Fri, 04 May 2018 07:16:42: 8000000 INFO @ Fri, 04 May 2018 07:16:47: 9000000 INFO @ Fri, 04 May 2018 07:16:47: 9000000 INFO @ Fri, 04 May 2018 07:16:49: 9000000 INFO @ Fri, 04 May 2018 07:16:54: 10000000 INFO @ Fri, 04 May 2018 07:16:54: 10000000 INFO @ Fri, 04 May 2018 07:16:57: 10000000 INFO @ Fri, 04 May 2018 07:17:01: 11000000 INFO @ Fri, 04 May 2018 07:17:01: 11000000 INFO @ Fri, 04 May 2018 07:17:04: 11000000 INFO @ Fri, 04 May 2018 07:17:08: 12000000 INFO @ Fri, 04 May 2018 07:17:08: 12000000 INFO @ Fri, 04 May 2018 07:17:11: 12000000 INFO @ Fri, 04 May 2018 07:17:15: 13000000 INFO @ Fri, 04 May 2018 07:17:15: 13000000 INFO @ Fri, 04 May 2018 07:17:18: 13000000 INFO @ Fri, 04 May 2018 07:17:21: 14000000 INFO @ Fri, 04 May 2018 07:17:22: 14000000 INFO @ Fri, 04 May 2018 07:17:25: 14000000 INFO @ Fri, 04 May 2018 07:17:28: 15000000 INFO @ Fri, 04 May 2018 07:17:28: 15000000 INFO @ Fri, 04 May 2018 07:17:32: 15000000 INFO @ Fri, 04 May 2018 07:17:35: 16000000 INFO @ Fri, 04 May 2018 07:17:35: 16000000 INFO @ Fri, 04 May 2018 07:17:39: 16000000 INFO @ Fri, 04 May 2018 07:17:42: 17000000 INFO @ Fri, 04 May 2018 07:17:42: 17000000 INFO @ Fri, 04 May 2018 07:17:47: 17000000 INFO @ Fri, 04 May 2018 07:17:49: 18000000 INFO @ Fri, 04 May 2018 07:17:49: 18000000 INFO @ Fri, 04 May 2018 07:17:54: 18000000 INFO @ Fri, 04 May 2018 07:17:56: #1 tag size is determined as 50 bps INFO @ Fri, 04 May 2018 07:17:56: #1 tag size = 50 INFO @ Fri, 04 May 2018 07:17:56: #1 total tags in treatment: 18987669 INFO @ Fri, 04 May 2018 07:17:56: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:17:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:17:56: #1 tag size is determined as 50 bps INFO @ Fri, 04 May 2018 07:17:56: #1 tag size = 50 INFO @ Fri, 04 May 2018 07:17:56: #1 total tags in treatment: 18987669 INFO @ Fri, 04 May 2018 07:17:56: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:17:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:17:56: #1 tags after filtering in treatment: 18987669 INFO @ Fri, 04 May 2018 07:17:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:17:56: #1 finished! INFO @ Fri, 04 May 2018 07:17:56: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:17:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:17:56: #1 tags after filtering in treatment: 18987669 INFO @ Fri, 04 May 2018 07:17:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:17:56: #1 finished! INFO @ Fri, 04 May 2018 07:17:56: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:17:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:17:57: #2 number of paired peaks: 10 WARNING @ Fri, 04 May 2018 07:17:57: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:17:57: Process for pairing-model is terminated! cat: SRX3011226.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3011226.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011226.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011226.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 04 May 2018 07:17:57: #2 number of paired peaks: 10 WARNING @ Fri, 04 May 2018 07:17:57: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:17:57: Process for pairing-model is terminated! cat: SRX3011226.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3011226.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011226.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011226.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 04 May 2018 07:18:01: #1 tag size is determined as 50 bps INFO @ Fri, 04 May 2018 07:18:01: #1 tag size = 50 INFO @ Fri, 04 May 2018 07:18:01: #1 total tags in treatment: 18987669 INFO @ Fri, 04 May 2018 07:18:01: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:18:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:18:01: #1 tags after filtering in treatment: 18987669 INFO @ Fri, 04 May 2018 07:18:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:18:01: #1 finished! INFO @ Fri, 04 May 2018 07:18:01: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:18:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:18:02: #2 number of paired peaks: 10 WARNING @ Fri, 04 May 2018 07:18:02: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:18:02: Process for pairing-model is terminated! cat: SRX3011226.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3011226.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011226.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3011226.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。