Job ID = 1295005 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 57,437,977 reads read : 114,875,954 reads written : 114,875,954 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:29:33 57437977 reads; of these: 57437977 (100.00%) were paired; of these: 9425920 (16.41%) aligned concordantly 0 times 36505232 (63.56%) aligned concordantly exactly 1 time 11506825 (20.03%) aligned concordantly >1 times ---- 9425920 pairs aligned concordantly 0 times; of these: 1301107 (13.80%) aligned discordantly 1 time ---- 8124813 pairs aligned 0 times concordantly or discordantly; of these: 16249626 mates make up the pairs; of these: 9992015 (61.49%) aligned 0 times 3998819 (24.61%) aligned exactly 1 time 2258792 (13.90%) aligned >1 times 91.30% overall alignment rate Time searching: 01:29:33 Overall time: 01:29:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 10168095 / 48254246 = 0.2107 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:32:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:32:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:32:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:32:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:32:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:32:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:32:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:32:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:32:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:32:26: 1000000 INFO @ Mon, 03 Jun 2019 13:32:27: 1000000 INFO @ Mon, 03 Jun 2019 13:32:27: 1000000 INFO @ Mon, 03 Jun 2019 13:32:32: 2000000 INFO @ Mon, 03 Jun 2019 13:32:36: 2000000 INFO @ Mon, 03 Jun 2019 13:32:37: 2000000 INFO @ Mon, 03 Jun 2019 13:32:40: 3000000 INFO @ Mon, 03 Jun 2019 13:32:45: 3000000 INFO @ Mon, 03 Jun 2019 13:32:45: 3000000 INFO @ Mon, 03 Jun 2019 13:32:47: 4000000 INFO @ Mon, 03 Jun 2019 13:32:54: 5000000 INFO @ Mon, 03 Jun 2019 13:32:54: 4000000 INFO @ Mon, 03 Jun 2019 13:32:54: 4000000 INFO @ Mon, 03 Jun 2019 13:33:01: 6000000 INFO @ Mon, 03 Jun 2019 13:33:03: 5000000 INFO @ Mon, 03 Jun 2019 13:33:03: 5000000 INFO @ Mon, 03 Jun 2019 13:33:08: 7000000 INFO @ Mon, 03 Jun 2019 13:33:10: 6000000 INFO @ Mon, 03 Jun 2019 13:33:11: 6000000 INFO @ Mon, 03 Jun 2019 13:33:15: 8000000 INFO @ Mon, 03 Jun 2019 13:33:18: 7000000 INFO @ Mon, 03 Jun 2019 13:33:19: 7000000 INFO @ Mon, 03 Jun 2019 13:33:22: 9000000 INFO @ Mon, 03 Jun 2019 13:33:26: 8000000 INFO @ Mon, 03 Jun 2019 13:33:28: 8000000 INFO @ Mon, 03 Jun 2019 13:33:29: 10000000 INFO @ Mon, 03 Jun 2019 13:33:34: 9000000 INFO @ Mon, 03 Jun 2019 13:33:36: 11000000 INFO @ Mon, 03 Jun 2019 13:33:37: 9000000 INFO @ Mon, 03 Jun 2019 13:33:42: 10000000 INFO @ Mon, 03 Jun 2019 13:33:43: 12000000 INFO @ Mon, 03 Jun 2019 13:33:45: 10000000 INFO @ Mon, 03 Jun 2019 13:33:49: 11000000 INFO @ Mon, 03 Jun 2019 13:33:49: 13000000 INFO @ Mon, 03 Jun 2019 13:33:53: 11000000 INFO @ Mon, 03 Jun 2019 13:33:56: 14000000 INFO @ Mon, 03 Jun 2019 13:33:57: 12000000 INFO @ Mon, 03 Jun 2019 13:34:01: 12000000 INFO @ Mon, 03 Jun 2019 13:34:03: 15000000 INFO @ Mon, 03 Jun 2019 13:34:05: 13000000 INFO @ Mon, 03 Jun 2019 13:34:10: 16000000 INFO @ Mon, 03 Jun 2019 13:34:10: 13000000 INFO @ Mon, 03 Jun 2019 13:34:13: 14000000 INFO @ Mon, 03 Jun 2019 13:34:16: 17000000 INFO @ Mon, 03 Jun 2019 13:34:18: 14000000 INFO @ Mon, 03 Jun 2019 13:34:20: 15000000 INFO @ Mon, 03 Jun 2019 13:34:24: 18000000 INFO @ Mon, 03 Jun 2019 13:34:26: 15000000 INFO @ Mon, 03 Jun 2019 13:34:28: 16000000 INFO @ Mon, 03 Jun 2019 13:34:31: 19000000 INFO @ Mon, 03 Jun 2019 13:34:34: 16000000 INFO @ Mon, 03 Jun 2019 13:34:36: 17000000 INFO @ Mon, 03 Jun 2019 13:34:38: 20000000 INFO @ Mon, 03 Jun 2019 13:34:43: 17000000 INFO @ Mon, 03 Jun 2019 13:34:43: 18000000 INFO @ Mon, 03 Jun 2019 13:34:44: 21000000 INFO @ Mon, 03 Jun 2019 13:34:50: 18000000 INFO @ Mon, 03 Jun 2019 13:34:51: 19000000 INFO @ Mon, 03 Jun 2019 13:34:51: 22000000 INFO @ Mon, 03 Jun 2019 13:34:56: 19000000 INFO @ Mon, 03 Jun 2019 13:34:58: 23000000 INFO @ Mon, 03 Jun 2019 13:34:59: 20000000 INFO @ Mon, 03 Jun 2019 13:35:04: 20000000 INFO @ Mon, 03 Jun 2019 13:35:04: 24000000 INFO @ Mon, 03 Jun 2019 13:35:06: 21000000 INFO @ Mon, 03 Jun 2019 13:35:11: 25000000 INFO @ Mon, 03 Jun 2019 13:35:12: 21000000 INFO @ Mon, 03 Jun 2019 13:35:14: 22000000 INFO @ Mon, 03 Jun 2019 13:35:17: 26000000 INFO @ Mon, 03 Jun 2019 13:35:20: 22000000 INFO @ Mon, 03 Jun 2019 13:35:21: 23000000 INFO @ Mon, 03 Jun 2019 13:35:24: 27000000 INFO @ Mon, 03 Jun 2019 13:35:29: 23000000 INFO @ Mon, 03 Jun 2019 13:35:29: 24000000 INFO @ Mon, 03 Jun 2019 13:35:30: 28000000 INFO @ Mon, 03 Jun 2019 13:35:36: 24000000 INFO @ Mon, 03 Jun 2019 13:35:36: 25000000 INFO @ Mon, 03 Jun 2019 13:35:36: 29000000 INFO @ Mon, 03 Jun 2019 13:35:43: 25000000 INFO @ Mon, 03 Jun 2019 13:35:43: 26000000 INFO @ Mon, 03 Jun 2019 13:35:44: 30000000 INFO @ Mon, 03 Jun 2019 13:35:50: 31000000 INFO @ Mon, 03 Jun 2019 13:35:51: 27000000 INFO @ Mon, 03 Jun 2019 13:35:51: 26000000 INFO @ Mon, 03 Jun 2019 13:35:57: 32000000 INFO @ Mon, 03 Jun 2019 13:35:58: 28000000 INFO @ Mon, 03 Jun 2019 13:35:59: 27000000 INFO @ Mon, 03 Jun 2019 13:36:03: 33000000 INFO @ Mon, 03 Jun 2019 13:36:05: 29000000 INFO @ Mon, 03 Jun 2019 13:36:07: 28000000 INFO @ Mon, 03 Jun 2019 13:36:10: 34000000 INFO @ Mon, 03 Jun 2019 13:36:13: 30000000 INFO @ Mon, 03 Jun 2019 13:36:14: 29000000 INFO @ Mon, 03 Jun 2019 13:36:16: 35000000 INFO @ Mon, 03 Jun 2019 13:36:20: 31000000 INFO @ Mon, 03 Jun 2019 13:36:22: 30000000 INFO @ Mon, 03 Jun 2019 13:36:23: 36000000 INFO @ Mon, 03 Jun 2019 13:36:28: 32000000 INFO @ Mon, 03 Jun 2019 13:36:29: 37000000 INFO @ Mon, 03 Jun 2019 13:36:30: 31000000 INFO @ Mon, 03 Jun 2019 13:36:35: 33000000 INFO @ Mon, 03 Jun 2019 13:36:36: 38000000 INFO @ Mon, 03 Jun 2019 13:36:38: 32000000 INFO @ Mon, 03 Jun 2019 13:36:42: 39000000 INFO @ Mon, 03 Jun 2019 13:36:43: 34000000 INFO @ Mon, 03 Jun 2019 13:36:46: 33000000 INFO @ Mon, 03 Jun 2019 13:36:48: 40000000 INFO @ Mon, 03 Jun 2019 13:36:49: 35000000 INFO @ Mon, 03 Jun 2019 13:36:53: 34000000 INFO @ Mon, 03 Jun 2019 13:36:55: 41000000 INFO @ Mon, 03 Jun 2019 13:36:56: 36000000 INFO @ Mon, 03 Jun 2019 13:37:01: 35000000 INFO @ Mon, 03 Jun 2019 13:37:02: 42000000 INFO @ Mon, 03 Jun 2019 13:37:03: 37000000 INFO @ Mon, 03 Jun 2019 13:37:08: 36000000 INFO @ Mon, 03 Jun 2019 13:37:09: 43000000 INFO @ Mon, 03 Jun 2019 13:37:10: 38000000 INFO @ Mon, 03 Jun 2019 13:37:15: 44000000 INFO @ Mon, 03 Jun 2019 13:37:16: 37000000 INFO @ Mon, 03 Jun 2019 13:37:18: 39000000 INFO @ Mon, 03 Jun 2019 13:37:22: 45000000 INFO @ Mon, 03 Jun 2019 13:37:25: 38000000 INFO @ Mon, 03 Jun 2019 13:37:25: 40000000 INFO @ Mon, 03 Jun 2019 13:37:28: 46000000 INFO @ Mon, 03 Jun 2019 13:37:33: 41000000 INFO @ Mon, 03 Jun 2019 13:37:33: 39000000 INFO @ Mon, 03 Jun 2019 13:37:34: 47000000 INFO @ Mon, 03 Jun 2019 13:37:40: 42000000 INFO @ Mon, 03 Jun 2019 13:37:40: 40000000 INFO @ Mon, 03 Jun 2019 13:37:41: 48000000 INFO @ Mon, 03 Jun 2019 13:37:47: 49000000 INFO @ Mon, 03 Jun 2019 13:37:47: 43000000 INFO @ Mon, 03 Jun 2019 13:37:48: 41000000 INFO @ Mon, 03 Jun 2019 13:37:54: 50000000 INFO @ Mon, 03 Jun 2019 13:37:55: 44000000 INFO @ Mon, 03 Jun 2019 13:37:56: 42000000 INFO @ Mon, 03 Jun 2019 13:38:00: 51000000 INFO @ Mon, 03 Jun 2019 13:38:02: 45000000 INFO @ Mon, 03 Jun 2019 13:38:03: 43000000 INFO @ Mon, 03 Jun 2019 13:38:06: 52000000 INFO @ Mon, 03 Jun 2019 13:38:10: 46000000 INFO @ Mon, 03 Jun 2019 13:38:11: 44000000 INFO @ Mon, 03 Jun 2019 13:38:12: 53000000 INFO @ Mon, 03 Jun 2019 13:38:17: 47000000 INFO @ Mon, 03 Jun 2019 13:38:19: 45000000 INFO @ Mon, 03 Jun 2019 13:38:19: 54000000 INFO @ Mon, 03 Jun 2019 13:38:25: 48000000 INFO @ Mon, 03 Jun 2019 13:38:26: 55000000 INFO @ Mon, 03 Jun 2019 13:38:26: 46000000 INFO @ Mon, 03 Jun 2019 13:38:32: 49000000 INFO @ Mon, 03 Jun 2019 13:38:32: 56000000 INFO @ Mon, 03 Jun 2019 13:38:34: 47000000 INFO @ Mon, 03 Jun 2019 13:38:39: 57000000 INFO @ Mon, 03 Jun 2019 13:38:39: 50000000 INFO @ Mon, 03 Jun 2019 13:38:42: 48000000 INFO @ Mon, 03 Jun 2019 13:38:45: 58000000 INFO @ Mon, 03 Jun 2019 13:38:46: 51000000 INFO @ Mon, 03 Jun 2019 13:38:50: 49000000 INFO @ Mon, 03 Jun 2019 13:38:51: 59000000 INFO @ Mon, 03 Jun 2019 13:38:54: 52000000 INFO @ Mon, 03 Jun 2019 13:38:58: 60000000 INFO @ Mon, 03 Jun 2019 13:38:58: 50000000 INFO @ Mon, 03 Jun 2019 13:39:01: 53000000 INFO @ Mon, 03 Jun 2019 13:39:04: 61000000 INFO @ Mon, 03 Jun 2019 13:39:05: 51000000 INFO @ Mon, 03 Jun 2019 13:39:08: 54000000 INFO @ Mon, 03 Jun 2019 13:39:11: 62000000 INFO @ Mon, 03 Jun 2019 13:39:13: 52000000 INFO @ Mon, 03 Jun 2019 13:39:15: 55000000 INFO @ Mon, 03 Jun 2019 13:39:17: 63000000 INFO @ Mon, 03 Jun 2019 13:39:20: 53000000 INFO @ Mon, 03 Jun 2019 13:39:23: 56000000 INFO @ Mon, 03 Jun 2019 13:39:23: 64000000 INFO @ Mon, 03 Jun 2019 13:39:28: 54000000 INFO @ Mon, 03 Jun 2019 13:39:29: 65000000 INFO @ Mon, 03 Jun 2019 13:39:30: 57000000 INFO @ Mon, 03 Jun 2019 13:39:36: 55000000 INFO @ Mon, 03 Jun 2019 13:39:36: 66000000 INFO @ Mon, 03 Jun 2019 13:39:37: 58000000 INFO @ Mon, 03 Jun 2019 13:39:43: 56000000 INFO @ Mon, 03 Jun 2019 13:39:43: 67000000 INFO @ Mon, 03 Jun 2019 13:39:45: 59000000 INFO @ Mon, 03 Jun 2019 13:39:50: 68000000 INFO @ Mon, 03 Jun 2019 13:39:51: 57000000 INFO @ Mon, 03 Jun 2019 13:39:52: 60000000 INFO @ Mon, 03 Jun 2019 13:39:56: 69000000 INFO @ Mon, 03 Jun 2019 13:39:58: 58000000 INFO @ Mon, 03 Jun 2019 13:39:59: 61000000 INFO @ Mon, 03 Jun 2019 13:40:02: 70000000 INFO @ Mon, 03 Jun 2019 13:40:06: 62000000 INFO @ Mon, 03 Jun 2019 13:40:07: 59000000 INFO @ Mon, 03 Jun 2019 13:40:09: 71000000 INFO @ Mon, 03 Jun 2019 13:40:14: 63000000 INFO @ Mon, 03 Jun 2019 13:40:15: 60000000 INFO @ Mon, 03 Jun 2019 13:40:15: 72000000 INFO @ Mon, 03 Jun 2019 13:40:21: 64000000 INFO @ Mon, 03 Jun 2019 13:40:21: 73000000 INFO @ Mon, 03 Jun 2019 13:40:23: 61000000 INFO @ Mon, 03 Jun 2019 13:40:28: 74000000 INFO @ Mon, 03 Jun 2019 13:40:28: 65000000 INFO @ Mon, 03 Jun 2019 13:40:31: 62000000 INFO @ Mon, 03 Jun 2019 13:40:34: 75000000 INFO @ Mon, 03 Jun 2019 13:40:36: 66000000 INFO @ Mon, 03 Jun 2019 13:40:39: 63000000 INFO @ Mon, 03 Jun 2019 13:40:40: 76000000 INFO @ Mon, 03 Jun 2019 13:40:43: 67000000 INFO @ Mon, 03 Jun 2019 13:40:47: 77000000 INFO @ Mon, 03 Jun 2019 13:40:47: 64000000 INFO @ Mon, 03 Jun 2019 13:40:51: 68000000 INFO @ Mon, 03 Jun 2019 13:40:53: 78000000 INFO @ Mon, 03 Jun 2019 13:40:55: 65000000 INFO @ Mon, 03 Jun 2019 13:40:58: 69000000 INFO @ Mon, 03 Jun 2019 13:41:00: 79000000 INFO @ Mon, 03 Jun 2019 13:41:03: 66000000 INFO @ Mon, 03 Jun 2019 13:41:06: 70000000 INFO @ Mon, 03 Jun 2019 13:41:07: 80000000 INFO @ Mon, 03 Jun 2019 13:41:11: 67000000 INFO @ Mon, 03 Jun 2019 13:41:13: 71000000 INFO @ Mon, 03 Jun 2019 13:41:13: 81000000 INFO @ Mon, 03 Jun 2019 13:41:19: 68000000 INFO @ Mon, 03 Jun 2019 13:41:19: 82000000 INFO @ Mon, 03 Jun 2019 13:41:20: 72000000 INFO @ Mon, 03 Jun 2019 13:41:26: 83000000 INFO @ Mon, 03 Jun 2019 13:41:27: 69000000 INFO @ Mon, 03 Jun 2019 13:41:28: 73000000 INFO @ Mon, 03 Jun 2019 13:41:32: 84000000 INFO @ Mon, 03 Jun 2019 13:41:35: 74000000 INFO @ Mon, 03 Jun 2019 13:41:36: 70000000 INFO @ Mon, 03 Jun 2019 13:41:36: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 13:41:36: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 13:41:36: #1 total tags in treatment: 37851470 INFO @ Mon, 03 Jun 2019 13:41:36: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:41:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:41:37: #1 tags after filtering in treatment: 22287398 INFO @ Mon, 03 Jun 2019 13:41:37: #1 Redundant rate of treatment: 0.41 INFO @ Mon, 03 Jun 2019 13:41:37: #1 finished! INFO @ Mon, 03 Jun 2019 13:41:37: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:41:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:41:39: #2 number of paired peaks: 18 WARNING @ Mon, 03 Jun 2019 13:41:39: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:41:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:41:42: 75000000 INFO @ Mon, 03 Jun 2019 13:41:44: 71000000 INFO @ Mon, 03 Jun 2019 13:41:49: 76000000 INFO @ Mon, 03 Jun 2019 13:41:52: 72000000 INFO @ Mon, 03 Jun 2019 13:41:57: 77000000 INFO @ Mon, 03 Jun 2019 13:42:00: 73000000 INFO @ Mon, 03 Jun 2019 13:42:04: 78000000 INFO @ Mon, 03 Jun 2019 13:42:08: 74000000 INFO @ Mon, 03 Jun 2019 13:42:11: 79000000 INFO @ Mon, 03 Jun 2019 13:42:16: 75000000 INFO @ Mon, 03 Jun 2019 13:42:18: 80000000 INFO @ Mon, 03 Jun 2019 13:42:23: 76000000 INFO @ Mon, 03 Jun 2019 13:42:25: 81000000 INFO @ Mon, 03 Jun 2019 13:42:31: 77000000 INFO @ Mon, 03 Jun 2019 13:42:33: 82000000 INFO @ Mon, 03 Jun 2019 13:42:39: 78000000 INFO @ Mon, 03 Jun 2019 13:42:40: 83000000 INFO @ Mon, 03 Jun 2019 13:42:46: 79000000 INFO @ Mon, 03 Jun 2019 13:42:47: 84000000 INFO @ Mon, 03 Jun 2019 13:42:52: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 13:42:52: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 13:42:52: #1 total tags in treatment: 37851470 INFO @ Mon, 03 Jun 2019 13:42:52: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:42:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:42:53: #1 tags after filtering in treatment: 22287398 INFO @ Mon, 03 Jun 2019 13:42:53: #1 Redundant rate of treatment: 0.41 INFO @ Mon, 03 Jun 2019 13:42:53: #1 finished! INFO @ Mon, 03 Jun 2019 13:42:53: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:42:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:42:54: 80000000 INFO @ Mon, 03 Jun 2019 13:42:54: #2 number of paired peaks: 18 WARNING @ Mon, 03 Jun 2019 13:42:54: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:42:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:43:02: 81000000 INFO @ Mon, 03 Jun 2019 13:43:10: 82000000 INFO @ Mon, 03 Jun 2019 13:43:18: 83000000 INFO @ Mon, 03 Jun 2019 13:43:26: 84000000 INFO @ Mon, 03 Jun 2019 13:43:31: #1 tag size is determined as 24 bps INFO @ Mon, 03 Jun 2019 13:43:31: #1 tag size = 24 INFO @ Mon, 03 Jun 2019 13:43:31: #1 total tags in treatment: 37851470 INFO @ Mon, 03 Jun 2019 13:43:31: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:43:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:43:32: #1 tags after filtering in treatment: 22287398 INFO @ Mon, 03 Jun 2019 13:43:32: #1 Redundant rate of treatment: 0.41 INFO @ Mon, 03 Jun 2019 13:43:32: #1 finished! INFO @ Mon, 03 Jun 2019 13:43:32: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:43:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:43:33: #2 number of paired peaks: 18 WARNING @ Mon, 03 Jun 2019 13:43:33: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:43:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX300939/SRX300939.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。