Job ID = 10924623 sra ファイルのダウンロード中... Completed: 3955234K bytes transferred in 200 seconds (161704K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 96848614 spots for /home/okishinya/chipatlas/results/dm3/SRX2998860/SRR5820982.sra Written 96848614 spots for /home/okishinya/chipatlas/results/dm3/SRX2998860/SRR5820982.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:39:33 96848614 reads; of these: 96848614 (100.00%) were unpaired; of these: 2545463 (2.63%) aligned 0 times 66070637 (68.22%) aligned exactly 1 time 28232514 (29.15%) aligned >1 times 97.37% overall alignment rate Time searching: 00:39:33 Overall time: 00:39:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 39746742 / 94303151 = 0.4215 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 06 Aug 2018 11:32:45: # Command line: callpeak -t SRX2998860.bam -f BAM -g dm -n SRX2998860.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2998860.10 # format = BAM # ChIP-seq file = ['SRX2998860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 11:32:45: # Command line: callpeak -t SRX2998860.bam -f BAM -g dm -n SRX2998860.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2998860.05 # format = BAM # ChIP-seq file = ['SRX2998860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 11:32:45: # Command line: callpeak -t SRX2998860.bam -f BAM -g dm -n SRX2998860.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2998860.20 # format = BAM # ChIP-seq file = ['SRX2998860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 06 Aug 2018 11:32:45: #1 read tag files... INFO @ Mon, 06 Aug 2018 11:32:45: #1 read tag files... INFO @ Mon, 06 Aug 2018 11:32:45: #1 read tag files... INFO @ Mon, 06 Aug 2018 11:32:45: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 11:32:45: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 11:32:45: #1 read treatment tags... INFO @ Mon, 06 Aug 2018 11:32:51: 1000000 INFO @ Mon, 06 Aug 2018 11:32:52: 1000000 INFO @ Mon, 06 Aug 2018 11:32:52: 1000000 INFO @ Mon, 06 Aug 2018 11:32:58: 2000000 INFO @ Mon, 06 Aug 2018 11:32:58: 2000000 INFO @ Mon, 06 Aug 2018 11:32:58: 2000000 INFO @ Mon, 06 Aug 2018 11:33:04: 3000000 INFO @ Mon, 06 Aug 2018 11:33:05: 3000000 INFO @ Mon, 06 Aug 2018 11:33:05: 3000000 INFO @ Mon, 06 Aug 2018 11:33:11: 4000000 INFO @ Mon, 06 Aug 2018 11:33:12: 4000000 INFO @ Mon, 06 Aug 2018 11:33:12: 4000000 INFO @ Mon, 06 Aug 2018 11:33:17: 5000000 INFO @ Mon, 06 Aug 2018 11:33:19: 5000000 INFO @ Mon, 06 Aug 2018 11:33:19: 5000000 INFO @ Mon, 06 Aug 2018 11:33:24: 6000000 INFO @ Mon, 06 Aug 2018 11:33:25: 6000000 INFO @ Mon, 06 Aug 2018 11:33:25: 6000000 INFO @ Mon, 06 Aug 2018 11:33:30: 7000000 INFO @ Mon, 06 Aug 2018 11:33:32: 7000000 INFO @ Mon, 06 Aug 2018 11:33:32: 7000000 INFO @ Mon, 06 Aug 2018 11:33:37: 8000000 INFO @ Mon, 06 Aug 2018 11:33:39: 8000000 INFO @ Mon, 06 Aug 2018 11:33:39: 8000000 INFO @ Mon, 06 Aug 2018 11:33:43: 9000000 INFO @ Mon, 06 Aug 2018 11:33:45: 9000000 INFO @ Mon, 06 Aug 2018 11:33:45: 9000000 INFO @ Mon, 06 Aug 2018 11:33:50: 10000000 INFO @ Mon, 06 Aug 2018 11:33:52: 10000000 INFO @ Mon, 06 Aug 2018 11:33:52: 10000000 INFO @ Mon, 06 Aug 2018 11:33:56: 11000000 INFO @ Mon, 06 Aug 2018 11:33:59: 11000000 INFO @ Mon, 06 Aug 2018 11:33:59: 11000000 INFO @ Mon, 06 Aug 2018 11:34:03: 12000000 INFO @ Mon, 06 Aug 2018 11:34:06: 12000000 INFO @ Mon, 06 Aug 2018 11:34:06: 12000000 INFO @ Mon, 06 Aug 2018 11:34:09: 13000000 INFO @ Mon, 06 Aug 2018 11:34:12: 13000000 INFO @ Mon, 06 Aug 2018 11:34:13: 13000000 INFO @ Mon, 06 Aug 2018 11:34:16: 14000000 INFO @ Mon, 06 Aug 2018 11:34:19: 14000000 INFO @ Mon, 06 Aug 2018 11:34:19: 14000000 INFO @ Mon, 06 Aug 2018 11:34:22: 15000000 INFO @ Mon, 06 Aug 2018 11:34:26: 15000000 INFO @ Mon, 06 Aug 2018 11:34:26: 15000000 INFO @ Mon, 06 Aug 2018 11:34:29: 16000000 INFO @ Mon, 06 Aug 2018 11:34:33: 16000000 INFO @ Mon, 06 Aug 2018 11:34:33: 16000000 INFO @ Mon, 06 Aug 2018 11:34:36: 17000000 INFO @ Mon, 06 Aug 2018 11:34:39: 17000000 INFO @ Mon, 06 Aug 2018 11:34:40: 17000000 INFO @ Mon, 06 Aug 2018 11:34:43: 18000000 INFO @ Mon, 06 Aug 2018 11:34:46: 18000000 INFO @ Mon, 06 Aug 2018 11:34:47: 18000000 INFO @ Mon, 06 Aug 2018 11:34:50: 19000000 INFO @ Mon, 06 Aug 2018 11:34:52: 19000000 INFO @ Mon, 06 Aug 2018 11:34:54: 19000000 INFO @ Mon, 06 Aug 2018 11:34:57: 20000000 INFO @ Mon, 06 Aug 2018 11:34:59: 20000000 INFO @ Mon, 06 Aug 2018 11:35:01: 20000000 INFO @ Mon, 06 Aug 2018 11:35:04: 21000000 INFO @ Mon, 06 Aug 2018 11:35:06: 21000000 INFO @ Mon, 06 Aug 2018 11:35:08: 21000000 INFO @ Mon, 06 Aug 2018 11:35:10: 22000000 INFO @ Mon, 06 Aug 2018 11:35:13: 22000000 INFO @ Mon, 06 Aug 2018 11:35:15: 22000000 INFO @ Mon, 06 Aug 2018 11:35:17: 23000000 INFO @ Mon, 06 Aug 2018 11:35:20: 23000000 INFO @ Mon, 06 Aug 2018 11:35:22: 23000000 INFO @ Mon, 06 Aug 2018 11:35:23: 24000000 INFO @ Mon, 06 Aug 2018 11:35:28: 24000000 INFO @ Mon, 06 Aug 2018 11:35:28: 24000000 INFO @ Mon, 06 Aug 2018 11:35:30: 25000000 INFO @ Mon, 06 Aug 2018 11:35:35: 25000000 INFO @ Mon, 06 Aug 2018 11:35:35: 25000000 INFO @ Mon, 06 Aug 2018 11:35:36: 26000000 INFO @ Mon, 06 Aug 2018 11:35:42: 26000000 INFO @ Mon, 06 Aug 2018 11:35:42: 26000000 INFO @ Mon, 06 Aug 2018 11:35:43: 27000000 INFO @ Mon, 06 Aug 2018 11:35:49: 27000000 INFO @ Mon, 06 Aug 2018 11:35:49: 27000000 INFO @ Mon, 06 Aug 2018 11:35:49: 28000000 INFO @ Mon, 06 Aug 2018 11:35:55: 28000000 INFO @ Mon, 06 Aug 2018 11:35:55: 28000000 INFO @ Mon, 06 Aug 2018 11:35:56: 29000000 INFO @ Mon, 06 Aug 2018 11:36:02: 29000000 INFO @ Mon, 06 Aug 2018 11:36:02: 30000000 INFO @ Mon, 06 Aug 2018 11:36:02: 29000000 INFO @ Mon, 06 Aug 2018 11:36:09: 31000000 INFO @ Mon, 06 Aug 2018 11:36:09: 30000000 INFO @ Mon, 06 Aug 2018 11:36:09: 30000000 INFO @ Mon, 06 Aug 2018 11:36:15: 31000000 INFO @ Mon, 06 Aug 2018 11:36:16: 32000000 INFO @ Mon, 06 Aug 2018 11:36:16: 31000000 INFO @ Mon, 06 Aug 2018 11:36:22: 33000000 INFO @ Mon, 06 Aug 2018 11:36:22: 32000000 INFO @ Mon, 06 Aug 2018 11:36:23: 32000000 INFO @ Mon, 06 Aug 2018 11:36:29: 34000000 INFO @ Mon, 06 Aug 2018 11:36:29: 33000000 INFO @ Mon, 06 Aug 2018 11:36:30: 33000000 INFO @ Mon, 06 Aug 2018 11:36:35: 35000000 INFO @ Mon, 06 Aug 2018 11:36:36: 34000000 INFO @ Mon, 06 Aug 2018 11:36:37: 34000000 INFO @ Mon, 06 Aug 2018 11:36:42: 36000000 INFO @ Mon, 06 Aug 2018 11:36:43: 35000000 INFO @ Mon, 06 Aug 2018 11:36:45: 35000000 INFO @ Mon, 06 Aug 2018 11:36:48: 37000000 INFO @ Mon, 06 Aug 2018 11:36:50: 36000000 INFO @ Mon, 06 Aug 2018 11:36:52: 36000000 INFO @ Mon, 06 Aug 2018 11:36:55: 38000000 INFO @ Mon, 06 Aug 2018 11:36:57: 37000000 INFO @ Mon, 06 Aug 2018 11:36:59: 37000000 INFO @ Mon, 06 Aug 2018 11:37:01: 39000000 INFO @ Mon, 06 Aug 2018 11:37:04: 38000000 INFO @ Mon, 06 Aug 2018 11:37:06: 38000000 INFO @ Mon, 06 Aug 2018 11:37:08: 40000000 INFO @ Mon, 06 Aug 2018 11:37:11: 39000000 INFO @ Mon, 06 Aug 2018 11:37:14: 39000000 INFO @ Mon, 06 Aug 2018 11:37:14: 41000000 INFO @ Mon, 06 Aug 2018 11:37:17: 40000000 INFO @ Mon, 06 Aug 2018 11:37:21: 42000000 INFO @ Mon, 06 Aug 2018 11:37:21: 40000000 INFO @ Mon, 06 Aug 2018 11:37:24: 41000000 INFO @ Mon, 06 Aug 2018 11:37:27: 43000000 INFO @ Mon, 06 Aug 2018 11:37:28: 41000000 INFO @ Mon, 06 Aug 2018 11:37:31: 42000000 INFO @ Mon, 06 Aug 2018 11:37:34: 44000000 INFO @ Mon, 06 Aug 2018 11:37:35: 42000000 INFO @ Mon, 06 Aug 2018 11:37:38: 43000000 INFO @ Mon, 06 Aug 2018 11:37:40: 45000000 INFO @ Mon, 06 Aug 2018 11:37:42: 43000000 INFO @ Mon, 06 Aug 2018 11:37:45: 44000000 INFO @ Mon, 06 Aug 2018 11:37:47: 46000000 INFO @ Mon, 06 Aug 2018 11:37:49: 44000000 INFO @ Mon, 06 Aug 2018 11:37:52: 45000000 INFO @ Mon, 06 Aug 2018 11:37:53: 47000000 INFO @ Mon, 06 Aug 2018 11:37:56: 45000000 INFO @ Mon, 06 Aug 2018 11:37:59: 46000000 INFO @ Mon, 06 Aug 2018 11:38:00: 48000000 INFO @ Mon, 06 Aug 2018 11:38:02: 46000000 INFO @ Mon, 06 Aug 2018 11:38:06: 47000000 INFO @ Mon, 06 Aug 2018 11:38:08: 49000000 INFO @ Mon, 06 Aug 2018 11:38:09: 47000000 INFO @ Mon, 06 Aug 2018 11:38:13: 48000000 INFO @ Mon, 06 Aug 2018 11:38:15: 50000000 INFO @ Mon, 06 Aug 2018 11:38:15: 48000000 INFO @ Mon, 06 Aug 2018 11:38:20: 49000000 INFO @ Mon, 06 Aug 2018 11:38:22: 49000000 INFO @ Mon, 06 Aug 2018 11:38:22: 51000000 INFO @ Mon, 06 Aug 2018 11:38:27: 50000000 INFO @ Mon, 06 Aug 2018 11:38:28: 50000000 INFO @ Mon, 06 Aug 2018 11:38:29: 52000000 INFO @ Mon, 06 Aug 2018 11:38:34: 51000000 INFO @ Mon, 06 Aug 2018 11:38:35: 51000000 INFO @ Mon, 06 Aug 2018 11:38:35: 53000000 INFO @ Mon, 06 Aug 2018 11:38:41: 52000000 INFO @ Mon, 06 Aug 2018 11:38:42: 54000000 INFO @ Mon, 06 Aug 2018 11:38:43: 52000000 INFO @ Mon, 06 Aug 2018 11:38:46: #1 tag size is determined as 49 bps INFO @ Mon, 06 Aug 2018 11:38:46: #1 tag size = 49 INFO @ Mon, 06 Aug 2018 11:38:46: #1 total tags in treatment: 54556409 INFO @ Mon, 06 Aug 2018 11:38:46: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 11:38:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 11:38:47: #1 tags after filtering in treatment: 54556409 INFO @ Mon, 06 Aug 2018 11:38:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 11:38:47: #1 finished! INFO @ Mon, 06 Aug 2018 11:38:47: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 11:38:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 11:38:47: 53000000 INFO @ Mon, 06 Aug 2018 11:38:50: 53000000 INFO @ Mon, 06 Aug 2018 11:38:51: #2 number of paired peaks: 0 WARNING @ Mon, 06 Aug 2018 11:38:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Aug 2018 11:38:51: Process for pairing-model is terminated! cat: SRX2998860.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2998860.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2998860.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2998860.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 06 Aug 2018 11:38:54: 54000000 INFO @ Mon, 06 Aug 2018 11:38:56: 54000000 INFO @ Mon, 06 Aug 2018 11:38:58: #1 tag size is determined as 49 bps INFO @ Mon, 06 Aug 2018 11:38:58: #1 tag size = 49 INFO @ Mon, 06 Aug 2018 11:38:58: #1 total tags in treatment: 54556409 INFO @ Mon, 06 Aug 2018 11:38:58: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 11:38:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 11:38:59: #1 tags after filtering in treatment: 54556409 INFO @ Mon, 06 Aug 2018 11:38:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 11:38:59: #1 finished! INFO @ Mon, 06 Aug 2018 11:38:59: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 11:38:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 11:39:00: #1 tag size is determined as 49 bps INFO @ Mon, 06 Aug 2018 11:39:00: #1 tag size = 49 INFO @ Mon, 06 Aug 2018 11:39:00: #1 total tags in treatment: 54556409 INFO @ Mon, 06 Aug 2018 11:39:00: #1 user defined the maximum tags... INFO @ Mon, 06 Aug 2018 11:39:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 06 Aug 2018 11:39:02: #1 tags after filtering in treatment: 54556409 INFO @ Mon, 06 Aug 2018 11:39:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 06 Aug 2018 11:39:02: #1 finished! INFO @ Mon, 06 Aug 2018 11:39:02: #2 Build Peak Model... INFO @ Mon, 06 Aug 2018 11:39:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 06 Aug 2018 11:39:03: #2 number of paired peaks: 0 WARNING @ Mon, 06 Aug 2018 11:39:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Aug 2018 11:39:03: Process for pairing-model is terminated! cat: SRX2998860.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2998860.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2998860.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2998860.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 06 Aug 2018 11:39:05: #2 number of paired peaks: 0 WARNING @ Mon, 06 Aug 2018 11:39:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 06 Aug 2018 11:39:05: Process for pairing-model is terminated! cat: SRX2998860.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2998860.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2998860.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2998860.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。