Job ID = 1294944 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-06-03T01:50:29 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-03T01:50:29 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra11/SRR/000849/SRR870197' 2019-06-03T01:50:29 fasterq-dump.2.9.6 err: invalid accession 'SRR870197' 2019-06-03T01:50:56 fasterq-dump.2.9.6 sys: connection not found while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download-internal.ncbi.nlm.nih.gov:443' 2019-06-03T01:50:56 fasterq-dump.2.9.6 err: connection not found while validating within network system module - error with https open 'https://sra-download-internal.ncbi.nlm.nih.gov/traces/sra11/SRR/000849/SRR870197' 2019-06-03T01:51:06 fasterq-dump.2.9.6 err: cmn_iter.c cmn_get_acc_type( 'SRR870197', 'NAME' ).VDBManagerOpenTableRead() -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) 2019-06-03T01:57:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T01:57:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 18,953,572 reads read : 18,953,572 reads written : 18,953,572 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:27 18953572 reads; of these: 18953572 (100.00%) were unpaired; of these: 973870 (5.14%) aligned 0 times 11627733 (61.35%) aligned exactly 1 time 6351969 (33.51%) aligned >1 times 94.86% overall alignment rate Time searching: 00:09:27 Overall time: 00:09:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3038541 / 17979702 = 0.1690 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 11:16:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 11:16:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 11:16:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 11:16:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 11:16:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 11:16:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 11:16:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 11:16:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 11:16:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 11:17:03: 1000000 INFO @ Mon, 03 Jun 2019 11:17:03: 1000000 INFO @ Mon, 03 Jun 2019 11:17:03: 1000000 INFO @ Mon, 03 Jun 2019 11:17:11: 2000000 INFO @ Mon, 03 Jun 2019 11:17:12: 2000000 INFO @ Mon, 03 Jun 2019 11:17:12: 2000000 INFO @ Mon, 03 Jun 2019 11:17:20: 3000000 INFO @ Mon, 03 Jun 2019 11:17:21: 3000000 INFO @ Mon, 03 Jun 2019 11:17:21: 3000000 INFO @ Mon, 03 Jun 2019 11:17:28: 4000000 INFO @ Mon, 03 Jun 2019 11:17:29: 4000000 INFO @ Mon, 03 Jun 2019 11:17:29: 4000000 INFO @ Mon, 03 Jun 2019 11:17:37: 5000000 INFO @ Mon, 03 Jun 2019 11:17:38: 5000000 INFO @ Mon, 03 Jun 2019 11:17:38: 5000000 INFO @ Mon, 03 Jun 2019 11:17:45: 6000000 INFO @ Mon, 03 Jun 2019 11:17:46: 6000000 INFO @ Mon, 03 Jun 2019 11:17:46: 6000000 INFO @ Mon, 03 Jun 2019 11:17:53: 7000000 INFO @ Mon, 03 Jun 2019 11:17:55: 7000000 INFO @ Mon, 03 Jun 2019 11:17:55: 7000000 INFO @ Mon, 03 Jun 2019 11:18:02: 8000000 INFO @ Mon, 03 Jun 2019 11:18:03: 8000000 INFO @ Mon, 03 Jun 2019 11:18:03: 8000000 INFO @ Mon, 03 Jun 2019 11:18:10: 9000000 INFO @ Mon, 03 Jun 2019 11:18:12: 9000000 INFO @ Mon, 03 Jun 2019 11:18:12: 9000000 INFO @ Mon, 03 Jun 2019 11:18:18: 10000000 INFO @ Mon, 03 Jun 2019 11:18:21: 10000000 INFO @ Mon, 03 Jun 2019 11:18:21: 10000000 INFO @ Mon, 03 Jun 2019 11:18:27: 11000000 INFO @ Mon, 03 Jun 2019 11:18:29: 11000000 INFO @ Mon, 03 Jun 2019 11:18:29: 11000000 INFO @ Mon, 03 Jun 2019 11:18:35: 12000000 INFO @ Mon, 03 Jun 2019 11:18:37: 12000000 INFO @ Mon, 03 Jun 2019 11:18:37: 12000000 INFO @ Mon, 03 Jun 2019 11:18:43: 13000000 INFO @ Mon, 03 Jun 2019 11:18:46: 13000000 INFO @ Mon, 03 Jun 2019 11:18:46: 13000000 INFO @ Mon, 03 Jun 2019 11:18:51: 14000000 INFO @ Mon, 03 Jun 2019 11:18:54: 14000000 INFO @ Mon, 03 Jun 2019 11:18:54: 14000000 INFO @ Mon, 03 Jun 2019 11:18:59: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 11:18:59: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 11:18:59: #1 total tags in treatment: 14941161 INFO @ Mon, 03 Jun 2019 11:18:59: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:18:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:18:59: #1 tags after filtering in treatment: 14941161 INFO @ Mon, 03 Jun 2019 11:18:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 11:18:59: #1 finished! INFO @ Mon, 03 Jun 2019 11:18:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:18:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:19:01: #2 number of paired peaks: 427 WARNING @ Mon, 03 Jun 2019 11:19:01: Fewer paired peaks (427) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 427 pairs to build model! INFO @ Mon, 03 Jun 2019 11:19:01: start model_add_line... INFO @ Mon, 03 Jun 2019 11:19:01: start X-correlation... INFO @ Mon, 03 Jun 2019 11:19:01: end of X-cor INFO @ Mon, 03 Jun 2019 11:19:01: #2 finished! INFO @ Mon, 03 Jun 2019 11:19:01: #2 predicted fragment length is 44 bps INFO @ Mon, 03 Jun 2019 11:19:01: #2 alternative fragment length(s) may be 44 bps INFO @ Mon, 03 Jun 2019 11:19:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.05_model.r WARNING @ Mon, 03 Jun 2019 11:19:01: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:19:01: #2 You may need to consider one of the other alternative d(s): 44 WARNING @ Mon, 03 Jun 2019 11:19:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:19:01: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:19:01: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:19:02: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 11:19:02: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 11:19:02: #1 total tags in treatment: 14941161 INFO @ Mon, 03 Jun 2019 11:19:02: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:19:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:19:02: #1 tags after filtering in treatment: 14941161 INFO @ Mon, 03 Jun 2019 11:19:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 11:19:02: #1 finished! INFO @ Mon, 03 Jun 2019 11:19:02: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:19:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:19:02: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 11:19:02: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 11:19:02: #1 total tags in treatment: 14941161 INFO @ Mon, 03 Jun 2019 11:19:02: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:19:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:19:02: #1 tags after filtering in treatment: 14941161 INFO @ Mon, 03 Jun 2019 11:19:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 11:19:02: #1 finished! INFO @ Mon, 03 Jun 2019 11:19:02: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:19:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:19:03: #2 number of paired peaks: 427 WARNING @ Mon, 03 Jun 2019 11:19:03: Fewer paired peaks (427) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 427 pairs to build model! INFO @ Mon, 03 Jun 2019 11:19:03: start model_add_line... INFO @ Mon, 03 Jun 2019 11:19:04: start X-correlation... INFO @ Mon, 03 Jun 2019 11:19:04: end of X-cor INFO @ Mon, 03 Jun 2019 11:19:04: #2 finished! INFO @ Mon, 03 Jun 2019 11:19:04: #2 predicted fragment length is 44 bps INFO @ Mon, 03 Jun 2019 11:19:04: #2 alternative fragment length(s) may be 44 bps INFO @ Mon, 03 Jun 2019 11:19:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.20_model.r WARNING @ Mon, 03 Jun 2019 11:19:04: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:19:04: #2 You may need to consider one of the other alternative d(s): 44 WARNING @ Mon, 03 Jun 2019 11:19:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:19:04: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:19:04: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:19:04: #2 number of paired peaks: 427 WARNING @ Mon, 03 Jun 2019 11:19:04: Fewer paired peaks (427) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 427 pairs to build model! INFO @ Mon, 03 Jun 2019 11:19:04: start model_add_line... INFO @ Mon, 03 Jun 2019 11:19:04: start X-correlation... INFO @ Mon, 03 Jun 2019 11:19:04: end of X-cor INFO @ Mon, 03 Jun 2019 11:19:04: #2 finished! INFO @ Mon, 03 Jun 2019 11:19:04: #2 predicted fragment length is 44 bps INFO @ Mon, 03 Jun 2019 11:19:04: #2 alternative fragment length(s) may be 44 bps INFO @ Mon, 03 Jun 2019 11:19:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.10_model.r WARNING @ Mon, 03 Jun 2019 11:19:04: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:19:04: #2 You may need to consider one of the other alternative d(s): 44 WARNING @ Mon, 03 Jun 2019 11:19:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:19:04: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:19:04: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:19:40: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:19:43: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:19:43: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:19:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.05_peaks.xls INFO @ Mon, 03 Jun 2019 11:19:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:19:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.05_summits.bed INFO @ Mon, 03 Jun 2019 11:19:59: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (2210 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 11:20:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.20_peaks.xls INFO @ Mon, 03 Jun 2019 11:20:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:20:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.20_summits.bed INFO @ Mon, 03 Jun 2019 11:20:02: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (1550 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 11:20:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.10_peaks.xls INFO @ Mon, 03 Jun 2019 11:20:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:20:03: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX288008/SRX288008.10_summits.bed INFO @ Mon, 03 Jun 2019 11:20:03: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (1938 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。