Job ID = 1294928 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,570,684 reads read : 17,570,684 reads written : 17,570,684 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:38 17570684 reads; of these: 17570684 (100.00%) were unpaired; of these: 849997 (4.84%) aligned 0 times 11024874 (62.75%) aligned exactly 1 time 5695813 (32.42%) aligned >1 times 95.16% overall alignment rate Time searching: 00:08:38 Overall time: 00:08:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2898147 / 16720687 = 0.1733 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 11:05:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 11:05:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 11:05:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 11:05:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 11:05:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 11:05:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 11:05:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 11:05:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 11:05:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 11:05:50: 1000000 INFO @ Mon, 03 Jun 2019 11:05:51: 1000000 INFO @ Mon, 03 Jun 2019 11:05:53: 1000000 INFO @ Mon, 03 Jun 2019 11:05:58: 2000000 INFO @ Mon, 03 Jun 2019 11:06:00: 2000000 INFO @ Mon, 03 Jun 2019 11:06:03: 2000000 INFO @ Mon, 03 Jun 2019 11:06:06: 3000000 INFO @ Mon, 03 Jun 2019 11:06:08: 3000000 INFO @ Mon, 03 Jun 2019 11:06:14: 3000000 INFO @ Mon, 03 Jun 2019 11:06:14: 4000000 INFO @ Mon, 03 Jun 2019 11:06:17: 4000000 INFO @ Mon, 03 Jun 2019 11:06:21: 5000000 INFO @ Mon, 03 Jun 2019 11:06:24: 4000000 INFO @ Mon, 03 Jun 2019 11:06:25: 5000000 INFO @ Mon, 03 Jun 2019 11:06:29: 6000000 INFO @ Mon, 03 Jun 2019 11:06:34: 6000000 INFO @ Mon, 03 Jun 2019 11:06:34: 5000000 INFO @ Mon, 03 Jun 2019 11:06:37: 7000000 INFO @ Mon, 03 Jun 2019 11:06:42: 7000000 INFO @ Mon, 03 Jun 2019 11:06:44: 6000000 INFO @ Mon, 03 Jun 2019 11:06:45: 8000000 INFO @ Mon, 03 Jun 2019 11:06:51: 8000000 INFO @ Mon, 03 Jun 2019 11:06:52: 9000000 INFO @ Mon, 03 Jun 2019 11:06:54: 7000000 INFO @ Mon, 03 Jun 2019 11:06:59: 9000000 INFO @ Mon, 03 Jun 2019 11:07:00: 10000000 INFO @ Mon, 03 Jun 2019 11:07:04: 8000000 INFO @ Mon, 03 Jun 2019 11:07:07: 10000000 INFO @ Mon, 03 Jun 2019 11:07:08: 11000000 INFO @ Mon, 03 Jun 2019 11:07:15: 9000000 INFO @ Mon, 03 Jun 2019 11:07:16: 11000000 INFO @ Mon, 03 Jun 2019 11:07:16: 12000000 INFO @ Mon, 03 Jun 2019 11:07:25: 10000000 INFO @ Mon, 03 Jun 2019 11:07:25: 12000000 INFO @ Mon, 03 Jun 2019 11:07:25: 13000000 INFO @ Mon, 03 Jun 2019 11:07:31: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 11:07:31: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 11:07:31: #1 total tags in treatment: 13822540 INFO @ Mon, 03 Jun 2019 11:07:31: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:07:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:07:32: #1 tags after filtering in treatment: 13822540 INFO @ Mon, 03 Jun 2019 11:07:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 11:07:32: #1 finished! INFO @ Mon, 03 Jun 2019 11:07:32: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:07:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:07:33: 13000000 INFO @ Mon, 03 Jun 2019 11:07:33: #2 number of paired peaks: 467 WARNING @ Mon, 03 Jun 2019 11:07:33: Fewer paired peaks (467) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 467 pairs to build model! INFO @ Mon, 03 Jun 2019 11:07:33: start model_add_line... INFO @ Mon, 03 Jun 2019 11:07:33: start X-correlation... INFO @ Mon, 03 Jun 2019 11:07:33: end of X-cor INFO @ Mon, 03 Jun 2019 11:07:33: #2 finished! INFO @ Mon, 03 Jun 2019 11:07:33: #2 predicted fragment length is 41 bps INFO @ Mon, 03 Jun 2019 11:07:33: #2 alternative fragment length(s) may be 41 bps INFO @ Mon, 03 Jun 2019 11:07:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.10_model.r WARNING @ Mon, 03 Jun 2019 11:07:33: #2 Since the d (41) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:07:33: #2 You may need to consider one of the other alternative d(s): 41 WARNING @ Mon, 03 Jun 2019 11:07:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:07:33: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:07:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:07:35: 11000000 INFO @ Mon, 03 Jun 2019 11:07:40: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 11:07:40: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 11:07:40: #1 total tags in treatment: 13822540 INFO @ Mon, 03 Jun 2019 11:07:40: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:07:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:07:40: #1 tags after filtering in treatment: 13822540 INFO @ Mon, 03 Jun 2019 11:07:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 11:07:40: #1 finished! INFO @ Mon, 03 Jun 2019 11:07:40: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:07:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:07:41: #2 number of paired peaks: 467 WARNING @ Mon, 03 Jun 2019 11:07:41: Fewer paired peaks (467) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 467 pairs to build model! INFO @ Mon, 03 Jun 2019 11:07:41: start model_add_line... INFO @ Mon, 03 Jun 2019 11:07:41: start X-correlation... INFO @ Mon, 03 Jun 2019 11:07:41: end of X-cor INFO @ Mon, 03 Jun 2019 11:07:41: #2 finished! INFO @ Mon, 03 Jun 2019 11:07:41: #2 predicted fragment length is 41 bps INFO @ Mon, 03 Jun 2019 11:07:41: #2 alternative fragment length(s) may be 41 bps INFO @ Mon, 03 Jun 2019 11:07:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.05_model.r WARNING @ Mon, 03 Jun 2019 11:07:42: #2 Since the d (41) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:07:42: #2 You may need to consider one of the other alternative d(s): 41 WARNING @ Mon, 03 Jun 2019 11:07:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:07:42: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:07:42: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:07:45: 12000000 INFO @ Mon, 03 Jun 2019 11:07:56: 13000000 INFO @ Mon, 03 Jun 2019 11:08:04: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 11:08:04: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 11:08:04: #1 total tags in treatment: 13822540 INFO @ Mon, 03 Jun 2019 11:08:04: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 11:08:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 11:08:04: #1 tags after filtering in treatment: 13822540 INFO @ Mon, 03 Jun 2019 11:08:04: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 11:08:04: #1 finished! INFO @ Mon, 03 Jun 2019 11:08:04: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 11:08:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 11:08:05: #2 number of paired peaks: 467 WARNING @ Mon, 03 Jun 2019 11:08:05: Fewer paired peaks (467) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 467 pairs to build model! INFO @ Mon, 03 Jun 2019 11:08:05: start model_add_line... INFO @ Mon, 03 Jun 2019 11:08:05: start X-correlation... INFO @ Mon, 03 Jun 2019 11:08:05: end of X-cor INFO @ Mon, 03 Jun 2019 11:08:05: #2 finished! INFO @ Mon, 03 Jun 2019 11:08:05: #2 predicted fragment length is 41 bps INFO @ Mon, 03 Jun 2019 11:08:05: #2 alternative fragment length(s) may be 41 bps INFO @ Mon, 03 Jun 2019 11:08:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.20_model.r WARNING @ Mon, 03 Jun 2019 11:08:05: #2 Since the d (41) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 11:08:05: #2 You may need to consider one of the other alternative d(s): 41 WARNING @ Mon, 03 Jun 2019 11:08:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 11:08:05: #3 Call peaks... INFO @ Mon, 03 Jun 2019 11:08:05: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 11:08:10: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:08:18: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:08:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.10_peaks.xls INFO @ Mon, 03 Jun 2019 11:08:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:08:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.10_summits.bed INFO @ Mon, 03 Jun 2019 11:08:28: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1899 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 11:08:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.05_peaks.xls INFO @ Mon, 03 Jun 2019 11:08:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:08:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.05_summits.bed INFO @ Mon, 03 Jun 2019 11:08:36: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (2172 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 11:08:42: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 11:09:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.20_peaks.xls INFO @ Mon, 03 Jun 2019 11:09:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 11:09:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287995/SRX287995.20_summits.bed INFO @ Mon, 03 Jun 2019 11:09:00: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (1509 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。