Job ID = 1294910 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,809,114 reads read : 13,809,114 reads written : 13,809,114 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:32 13809114 reads; of these: 13809114 (100.00%) were unpaired; of these: 547885 (3.97%) aligned 0 times 8994325 (65.13%) aligned exactly 1 time 4266904 (30.90%) aligned >1 times 96.03% overall alignment rate Time searching: 00:06:32 Overall time: 00:06:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1852328 / 13261229 = 0.1397 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 10:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:55:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:55:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:55:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:55:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 10:55:30: #1 read tag files... INFO @ Mon, 03 Jun 2019 10:55:30: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 10:55:39: 1000000 INFO @ Mon, 03 Jun 2019 10:55:40: 1000000 INFO @ Mon, 03 Jun 2019 10:55:41: 1000000 INFO @ Mon, 03 Jun 2019 10:55:48: 2000000 INFO @ Mon, 03 Jun 2019 10:55:50: 2000000 INFO @ Mon, 03 Jun 2019 10:55:51: 2000000 INFO @ Mon, 03 Jun 2019 10:55:57: 3000000 INFO @ Mon, 03 Jun 2019 10:55:59: 3000000 INFO @ Mon, 03 Jun 2019 10:56:02: 3000000 INFO @ Mon, 03 Jun 2019 10:56:06: 4000000 INFO @ Mon, 03 Jun 2019 10:56:08: 4000000 INFO @ Mon, 03 Jun 2019 10:56:12: 4000000 INFO @ Mon, 03 Jun 2019 10:56:14: 5000000 INFO @ Mon, 03 Jun 2019 10:56:17: 5000000 INFO @ Mon, 03 Jun 2019 10:56:22: 5000000 INFO @ Mon, 03 Jun 2019 10:56:23: 6000000 INFO @ Mon, 03 Jun 2019 10:56:26: 6000000 INFO @ Mon, 03 Jun 2019 10:56:32: 7000000 INFO @ Mon, 03 Jun 2019 10:56:33: 6000000 INFO @ Mon, 03 Jun 2019 10:56:34: 7000000 INFO @ Mon, 03 Jun 2019 10:56:42: 8000000 INFO @ Mon, 03 Jun 2019 10:56:43: 7000000 INFO @ Mon, 03 Jun 2019 10:56:44: 8000000 INFO @ Mon, 03 Jun 2019 10:56:51: 9000000 INFO @ Mon, 03 Jun 2019 10:56:53: 9000000 INFO @ Mon, 03 Jun 2019 10:56:54: 8000000 INFO @ Mon, 03 Jun 2019 10:56:59: 10000000 INFO @ Mon, 03 Jun 2019 10:57:02: 10000000 INFO @ Mon, 03 Jun 2019 10:57:04: 9000000 INFO @ Mon, 03 Jun 2019 10:57:08: 11000000 INFO @ Mon, 03 Jun 2019 10:57:11: 11000000 INFO @ Mon, 03 Jun 2019 10:57:12: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 10:57:12: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 10:57:12: #1 total tags in treatment: 11408901 INFO @ Mon, 03 Jun 2019 10:57:12: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 10:57:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 10:57:12: #1 tags after filtering in treatment: 11408901 INFO @ Mon, 03 Jun 2019 10:57:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 10:57:12: #1 finished! INFO @ Mon, 03 Jun 2019 10:57:12: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 10:57:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 10:57:13: #2 number of paired peaks: 452 WARNING @ Mon, 03 Jun 2019 10:57:13: Fewer paired peaks (452) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 452 pairs to build model! INFO @ Mon, 03 Jun 2019 10:57:13: start model_add_line... INFO @ Mon, 03 Jun 2019 10:57:13: start X-correlation... INFO @ Mon, 03 Jun 2019 10:57:13: end of X-cor INFO @ Mon, 03 Jun 2019 10:57:13: #2 finished! INFO @ Mon, 03 Jun 2019 10:57:13: #2 predicted fragment length is 45 bps INFO @ Mon, 03 Jun 2019 10:57:13: #2 alternative fragment length(s) may be 45 bps INFO @ Mon, 03 Jun 2019 10:57:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.05_model.r WARNING @ Mon, 03 Jun 2019 10:57:13: #2 Since the d (45) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 10:57:13: #2 You may need to consider one of the other alternative d(s): 45 WARNING @ Mon, 03 Jun 2019 10:57:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 10:57:13: #3 Call peaks... INFO @ Mon, 03 Jun 2019 10:57:13: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 10:57:14: 10000000 INFO @ Mon, 03 Jun 2019 10:57:15: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 10:57:15: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 10:57:15: #1 total tags in treatment: 11408901 INFO @ Mon, 03 Jun 2019 10:57:15: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 10:57:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 10:57:15: #1 tags after filtering in treatment: 11408901 INFO @ Mon, 03 Jun 2019 10:57:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 10:57:15: #1 finished! INFO @ Mon, 03 Jun 2019 10:57:15: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 10:57:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 10:57:16: #2 number of paired peaks: 452 WARNING @ Mon, 03 Jun 2019 10:57:16: Fewer paired peaks (452) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 452 pairs to build model! INFO @ Mon, 03 Jun 2019 10:57:16: start model_add_line... INFO @ Mon, 03 Jun 2019 10:57:16: start X-correlation... INFO @ Mon, 03 Jun 2019 10:57:16: end of X-cor INFO @ Mon, 03 Jun 2019 10:57:16: #2 finished! INFO @ Mon, 03 Jun 2019 10:57:16: #2 predicted fragment length is 45 bps INFO @ Mon, 03 Jun 2019 10:57:16: #2 alternative fragment length(s) may be 45 bps INFO @ Mon, 03 Jun 2019 10:57:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.10_model.r WARNING @ Mon, 03 Jun 2019 10:57:16: #2 Since the d (45) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 10:57:16: #2 You may need to consider one of the other alternative d(s): 45 WARNING @ Mon, 03 Jun 2019 10:57:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 10:57:16: #3 Call peaks... INFO @ Mon, 03 Jun 2019 10:57:16: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 10:57:24: 11000000 INFO @ Mon, 03 Jun 2019 10:57:27: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 10:57:27: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 10:57:27: #1 total tags in treatment: 11408901 INFO @ Mon, 03 Jun 2019 10:57:27: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 10:57:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 10:57:28: #1 tags after filtering in treatment: 11408901 INFO @ Mon, 03 Jun 2019 10:57:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 10:57:28: #1 finished! INFO @ Mon, 03 Jun 2019 10:57:28: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 10:57:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 10:57:29: #2 number of paired peaks: 452 WARNING @ Mon, 03 Jun 2019 10:57:29: Fewer paired peaks (452) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 452 pairs to build model! INFO @ Mon, 03 Jun 2019 10:57:29: start model_add_line... INFO @ Mon, 03 Jun 2019 10:57:29: start X-correlation... INFO @ Mon, 03 Jun 2019 10:57:29: end of X-cor INFO @ Mon, 03 Jun 2019 10:57:29: #2 finished! INFO @ Mon, 03 Jun 2019 10:57:29: #2 predicted fragment length is 45 bps INFO @ Mon, 03 Jun 2019 10:57:29: #2 alternative fragment length(s) may be 45 bps INFO @ Mon, 03 Jun 2019 10:57:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.20_model.r WARNING @ Mon, 03 Jun 2019 10:57:29: #2 Since the d (45) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 10:57:29: #2 You may need to consider one of the other alternative d(s): 45 WARNING @ Mon, 03 Jun 2019 10:57:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 10:57:29: #3 Call peaks... INFO @ Mon, 03 Jun 2019 10:57:29: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 10:57:44: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 10:57:47: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 10:57:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.05_peaks.xls INFO @ Mon, 03 Jun 2019 10:57:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 10:57:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.05_summits.bed INFO @ Mon, 03 Jun 2019 10:57:59: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (2094 records, 4 fields): 7 millis INFO @ Mon, 03 Jun 2019 10:58:00: #3 Call peaks for each chromosome... CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 10:58:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.10_peaks.xls INFO @ Mon, 03 Jun 2019 10:58:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 10:58:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.10_summits.bed INFO @ Mon, 03 Jun 2019 10:58:02: Done! pass1 - making usageList (13 chroms): 2 millis pass2 - checking and writing primary data (1851 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 10:58:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.20_peaks.xls INFO @ Mon, 03 Jun 2019 10:58:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 10:58:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX287983/SRX287983.20_summits.bed INFO @ Mon, 03 Jun 2019 10:58:15: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1412 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。