Job ID = 6527873 SRX = SRX287974 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:45:29 prefetch.2.10.7: 1) Downloading 'SRR870163'... 2020-06-29T13:45:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:49:27 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:49:27 prefetch.2.10.7: 1) 'SRR870163' was downloaded successfully Read 17573762 spots for SRR870163/SRR870163.sra Written 17573762 spots for SRR870163/SRR870163.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:37 17573762 reads; of these: 17573762 (100.00%) were unpaired; of these: 943486 (5.37%) aligned 0 times 10817041 (61.55%) aligned exactly 1 time 5813235 (33.08%) aligned >1 times 94.63% overall alignment rate Time searching: 00:06:37 Overall time: 00:06:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1880949 / 16630276 = 0.1131 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:36: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:36: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:42: 1000000 INFO @ Mon, 29 Jun 2020 23:05:47: 2000000 INFO @ Mon, 29 Jun 2020 23:05:52: 3000000 INFO @ Mon, 29 Jun 2020 23:05:57: 4000000 INFO @ Mon, 29 Jun 2020 23:06:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:07: 6000000 INFO @ Mon, 29 Jun 2020 23:06:11: 1000000 INFO @ Mon, 29 Jun 2020 23:06:13: 7000000 INFO @ Mon, 29 Jun 2020 23:06:16: 2000000 INFO @ Mon, 29 Jun 2020 23:06:18: 8000000 INFO @ Mon, 29 Jun 2020 23:06:22: 3000000 INFO @ Mon, 29 Jun 2020 23:06:24: 9000000 INFO @ Mon, 29 Jun 2020 23:06:27: 4000000 INFO @ Mon, 29 Jun 2020 23:06:29: 10000000 INFO @ Mon, 29 Jun 2020 23:06:32: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:34: 11000000 INFO @ Mon, 29 Jun 2020 23:06:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:36: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:36: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:38: 6000000 INFO @ Mon, 29 Jun 2020 23:06:40: 12000000 INFO @ Mon, 29 Jun 2020 23:06:42: 1000000 INFO @ Mon, 29 Jun 2020 23:06:43: 7000000 INFO @ Mon, 29 Jun 2020 23:06:46: 13000000 INFO @ Mon, 29 Jun 2020 23:06:48: 2000000 INFO @ Mon, 29 Jun 2020 23:06:49: 8000000 INFO @ Mon, 29 Jun 2020 23:06:52: 14000000 INFO @ Mon, 29 Jun 2020 23:06:55: 3000000 INFO @ Mon, 29 Jun 2020 23:06:55: 9000000 INFO @ Mon, 29 Jun 2020 23:06:56: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:06:56: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:06:56: #1 total tags in treatment: 14749327 INFO @ Mon, 29 Jun 2020 23:06:56: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:06:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:06:57: #1 tags after filtering in treatment: 14749327 INFO @ Mon, 29 Jun 2020 23:06:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:06:57: #1 finished! INFO @ Mon, 29 Jun 2020 23:06:57: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:06:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:06:58: #2 number of paired peaks: 78 WARNING @ Mon, 29 Jun 2020 23:06:58: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:06:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:07:01: 4000000 INFO @ Mon, 29 Jun 2020 23:07:01: 10000000 INFO @ Mon, 29 Jun 2020 23:07:07: 11000000 INFO @ Mon, 29 Jun 2020 23:07:07: 5000000 INFO @ Mon, 29 Jun 2020 23:07:13: 12000000 INFO @ Mon, 29 Jun 2020 23:07:13: 6000000 INFO @ Mon, 29 Jun 2020 23:07:19: 13000000 INFO @ Mon, 29 Jun 2020 23:07:20: 7000000 INFO @ Mon, 29 Jun 2020 23:07:24: 14000000 INFO @ Mon, 29 Jun 2020 23:07:26: 8000000 INFO @ Mon, 29 Jun 2020 23:07:29: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:29: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:29: #1 total tags in treatment: 14749327 INFO @ Mon, 29 Jun 2020 23:07:29: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:29: #1 tags after filtering in treatment: 14749327 INFO @ Mon, 29 Jun 2020 23:07:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:29: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:29: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:30: #2 number of paired peaks: 78 WARNING @ Mon, 29 Jun 2020 23:07:30: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:07:32: 9000000 INFO @ Mon, 29 Jun 2020 23:07:38: 10000000 INFO @ Mon, 29 Jun 2020 23:07:44: 11000000 INFO @ Mon, 29 Jun 2020 23:07:50: 12000000 INFO @ Mon, 29 Jun 2020 23:07:57: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:08:03: 14000000 INFO @ Mon, 29 Jun 2020 23:08:08: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:08: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:08: #1 total tags in treatment: 14749327 INFO @ Mon, 29 Jun 2020 23:08:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:08: #1 tags after filtering in treatment: 14749327 INFO @ Mon, 29 Jun 2020 23:08:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:08: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:09: #2 number of paired peaks: 78 WARNING @ Mon, 29 Jun 2020 23:08:09: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287974/SRX287974.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。