Job ID = 6527872 SRX = SRX287973 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:42:15 prefetch.2.10.7: 1) Downloading 'SRR870162'... 2020-06-29T13:42:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:45:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:45:41 prefetch.2.10.7: 1) 'SRR870162' was downloaded successfully Read 17599879 spots for SRR870162/SRR870162.sra Written 17599879 spots for SRR870162/SRR870162.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 17599879 reads; of these: 17599879 (100.00%) were unpaired; of these: 889832 (5.06%) aligned 0 times 11503427 (65.36%) aligned exactly 1 time 5206620 (29.58%) aligned >1 times 94.94% overall alignment rate Time searching: 00:06:10 Overall time: 00:06:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1075853 / 16710047 = 0.0644 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:01:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:01:20: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:01:20: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:01:26: 1000000 INFO @ Mon, 29 Jun 2020 23:01:31: 2000000 INFO @ Mon, 29 Jun 2020 23:01:37: 3000000 INFO @ Mon, 29 Jun 2020 23:01:42: 4000000 INFO @ Mon, 29 Jun 2020 23:01:47: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:01:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:01:50: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:01:50: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:01:52: 6000000 INFO @ Mon, 29 Jun 2020 23:01:56: 1000000 INFO @ Mon, 29 Jun 2020 23:01:58: 7000000 INFO @ Mon, 29 Jun 2020 23:02:01: 2000000 INFO @ Mon, 29 Jun 2020 23:02:03: 8000000 INFO @ Mon, 29 Jun 2020 23:02:07: 3000000 INFO @ Mon, 29 Jun 2020 23:02:09: 9000000 INFO @ Mon, 29 Jun 2020 23:02:13: 4000000 INFO @ Mon, 29 Jun 2020 23:02:14: 10000000 INFO @ Mon, 29 Jun 2020 23:02:18: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:02:20: 11000000 INFO @ Mon, 29 Jun 2020 23:02:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:02:20: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:02:20: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:02:24: 6000000 INFO @ Mon, 29 Jun 2020 23:02:26: 12000000 INFO @ Mon, 29 Jun 2020 23:02:26: 1000000 INFO @ Mon, 29 Jun 2020 23:02:29: 7000000 INFO @ Mon, 29 Jun 2020 23:02:31: 13000000 INFO @ Mon, 29 Jun 2020 23:02:32: 2000000 INFO @ Mon, 29 Jun 2020 23:02:35: 8000000 INFO @ Mon, 29 Jun 2020 23:02:37: 14000000 INFO @ Mon, 29 Jun 2020 23:02:38: 3000000 INFO @ Mon, 29 Jun 2020 23:02:41: 9000000 INFO @ Mon, 29 Jun 2020 23:02:42: 15000000 INFO @ Mon, 29 Jun 2020 23:02:44: 4000000 INFO @ Mon, 29 Jun 2020 23:02:46: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:02:46: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:02:46: #1 total tags in treatment: 15634194 INFO @ Mon, 29 Jun 2020 23:02:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:02:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:02:46: #1 tags after filtering in treatment: 15634194 INFO @ Mon, 29 Jun 2020 23:02:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:02:46: #1 finished! INFO @ Mon, 29 Jun 2020 23:02:46: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:02:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:02:46: 10000000 INFO @ Mon, 29 Jun 2020 23:02:47: #2 number of paired peaks: 19 WARNING @ Mon, 29 Jun 2020 23:02:47: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:02:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:02:49: 5000000 INFO @ Mon, 29 Jun 2020 23:02:52: 11000000 INFO @ Mon, 29 Jun 2020 23:02:55: 6000000 INFO @ Mon, 29 Jun 2020 23:02:58: 12000000 INFO @ Mon, 29 Jun 2020 23:03:00: 7000000 INFO @ Mon, 29 Jun 2020 23:03:03: 13000000 INFO @ Mon, 29 Jun 2020 23:03:06: 8000000 INFO @ Mon, 29 Jun 2020 23:03:09: 14000000 INFO @ Mon, 29 Jun 2020 23:03:12: 9000000 INFO @ Mon, 29 Jun 2020 23:03:14: 15000000 INFO @ Mon, 29 Jun 2020 23:03:17: 10000000 INFO @ Mon, 29 Jun 2020 23:03:18: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:03:18: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:03:18: #1 total tags in treatment: 15634194 INFO @ Mon, 29 Jun 2020 23:03:18: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:03:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:03:18: #1 tags after filtering in treatment: 15634194 INFO @ Mon, 29 Jun 2020 23:03:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:03:18: #1 finished! INFO @ Mon, 29 Jun 2020 23:03:18: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:03:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:03:19: #2 number of paired peaks: 19 WARNING @ Mon, 29 Jun 2020 23:03:19: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:03:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:03:23: 11000000 INFO @ Mon, 29 Jun 2020 23:03:28: 12000000 INFO @ Mon, 29 Jun 2020 23:03:33: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:03:39: 14000000 INFO @ Mon, 29 Jun 2020 23:03:44: 15000000 INFO @ Mon, 29 Jun 2020 23:03:48: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:03:48: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:03:48: #1 total tags in treatment: 15634194 INFO @ Mon, 29 Jun 2020 23:03:48: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:03:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:03:48: #1 tags after filtering in treatment: 15634194 INFO @ Mon, 29 Jun 2020 23:03:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:03:48: #1 finished! INFO @ Mon, 29 Jun 2020 23:03:48: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:03:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:03:49: #2 number of paired peaks: 19 WARNING @ Mon, 29 Jun 2020 23:03:49: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:03:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287973/SRX287973.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。