Job ID = 9159291 sra ファイルのダウンロード中... Completed: 382341K bytes transferred in 6 seconds (501714K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 11846426 spots for /home/okishinya/chipatlas/results/dm3/SRX287970/SRR870159.sra Written 11846426 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:31 11846426 reads; of these: 11846426 (100.00%) were unpaired; of these: 644051 (5.44%) aligned 0 times 9741591 (82.23%) aligned exactly 1 time 1460784 (12.33%) aligned >1 times 94.56% overall alignment rate Time searching: 00:03:31 Overall time: 00:03:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 847661 / 11202375 = 0.0757 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 19:53:48: # Command line: callpeak -t SRX287970.bam -f BAM -g dm -n SRX287970.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX287970.20 # format = BAM # ChIP-seq file = ['SRX287970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:53:48: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:53:48: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:53:48: # Command line: callpeak -t SRX287970.bam -f BAM -g dm -n SRX287970.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX287970.10 # format = BAM # ChIP-seq file = ['SRX287970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:53:48: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:53:48: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:53:48: # Command line: callpeak -t SRX287970.bam -f BAM -g dm -n SRX287970.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX287970.05 # format = BAM # ChIP-seq file = ['SRX287970.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 19:53:48: #1 read tag files... INFO @ Tue, 27 Jun 2017 19:53:48: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 19:53:56: 1000000 INFO @ Tue, 27 Jun 2017 19:53:58: 1000000 INFO @ Tue, 27 Jun 2017 19:53:58: 1000000 INFO @ Tue, 27 Jun 2017 19:54:05: 2000000 INFO @ Tue, 27 Jun 2017 19:54:08: 2000000 INFO @ Tue, 27 Jun 2017 19:54:08: 2000000 INFO @ Tue, 27 Jun 2017 19:54:13: 3000000 INFO @ Tue, 27 Jun 2017 19:54:18: 3000000 INFO @ Tue, 27 Jun 2017 19:54:18: 3000000 INFO @ Tue, 27 Jun 2017 19:54:21: 4000000 INFO @ Tue, 27 Jun 2017 19:54:28: 4000000 INFO @ Tue, 27 Jun 2017 19:54:28: 4000000 INFO @ Tue, 27 Jun 2017 19:54:29: 5000000 INFO @ Tue, 27 Jun 2017 19:54:38: 5000000 INFO @ Tue, 27 Jun 2017 19:54:38: 5000000 INFO @ Tue, 27 Jun 2017 19:54:38: 6000000 INFO @ Tue, 27 Jun 2017 19:54:46: 7000000 INFO @ Tue, 27 Jun 2017 19:54:47: 6000000 INFO @ Tue, 27 Jun 2017 19:54:47: 6000000 INFO @ Tue, 27 Jun 2017 19:54:54: 8000000 INFO @ Tue, 27 Jun 2017 19:54:57: 7000000 INFO @ Tue, 27 Jun 2017 19:54:57: 7000000 INFO @ Tue, 27 Jun 2017 19:55:03: 9000000 INFO @ Tue, 27 Jun 2017 19:55:07: 8000000 INFO @ Tue, 27 Jun 2017 19:55:07: 8000000 INFO @ Tue, 27 Jun 2017 19:55:11: 10000000 INFO @ Tue, 27 Jun 2017 19:55:14: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 19:55:14: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 19:55:14: #1 total tags in treatment: 10354714 INFO @ Tue, 27 Jun 2017 19:55:14: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:55:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:55:14: #1 tags after filtering in treatment: 10354714 INFO @ Tue, 27 Jun 2017 19:55:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:55:14: #1 finished! INFO @ Tue, 27 Jun 2017 19:55:14: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:55:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:55:15: #2 number of paired peaks: 16 WARNING @ Tue, 27 Jun 2017 19:55:15: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:55:15: Process for pairing-model is terminated! cat: SRX287970.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX287970.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX287970.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX287970.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:55:17: 9000000 INFO @ Tue, 27 Jun 2017 19:55:17: 9000000 INFO @ Tue, 27 Jun 2017 19:55:27: 10000000 INFO @ Tue, 27 Jun 2017 19:55:27: 10000000 INFO @ Tue, 27 Jun 2017 19:55:31: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 19:55:31: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 19:55:31: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 19:55:31: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 19:55:31: #1 total tags in treatment: 10354714 INFO @ Tue, 27 Jun 2017 19:55:31: #1 total tags in treatment: 10354714 INFO @ Tue, 27 Jun 2017 19:55:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:55:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 19:55:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:55:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 19:55:31: #1 tags after filtering in treatment: 10354714 INFO @ Tue, 27 Jun 2017 19:55:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:55:31: #1 finished! INFO @ Tue, 27 Jun 2017 19:55:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:55:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:55:31: #1 tags after filtering in treatment: 10354714 INFO @ Tue, 27 Jun 2017 19:55:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 19:55:31: #1 finished! INFO @ Tue, 27 Jun 2017 19:55:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 19:55:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 19:55:32: #2 number of paired peaks: 16 WARNING @ Tue, 27 Jun 2017 19:55:32: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:55:32: Process for pairing-model is terminated! cat: SRX287970.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX287970.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX287970.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX287970.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 19:55:32: #2 number of paired peaks: 16 WARNING @ Tue, 27 Jun 2017 19:55:32: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 19:55:32: Process for pairing-model is terminated! cat: SRX287970.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX287970.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX287970.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX287970.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。