Job ID = 6527863 SRX = SRX287915 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:22:51 prefetch.2.10.7: 1) Downloading 'SRR870104'... 2020-06-29T13:22:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:24:55 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:24:55 prefetch.2.10.7: 1) 'SRR870104' was downloaded successfully Read 16042597 spots for SRR870104/SRR870104.sra Written 16042597 spots for SRR870104/SRR870104.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:25 16042597 reads; of these: 16042597 (100.00%) were unpaired; of these: 802873 (5.00%) aligned 0 times 11773615 (73.39%) aligned exactly 1 time 3466109 (21.61%) aligned >1 times 95.00% overall alignment rate Time searching: 00:05:25 Overall time: 00:05:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1043543 / 15239724 = 0.0685 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:41:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:41:24: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:41:24: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:41:29: 1000000 INFO @ Mon, 29 Jun 2020 22:41:35: 2000000 INFO @ Mon, 29 Jun 2020 22:41:40: 3000000 INFO @ Mon, 29 Jun 2020 22:41:45: 4000000 INFO @ Mon, 29 Jun 2020 22:41:50: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:41:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:41:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:41:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:41:55: 6000000 INFO @ Mon, 29 Jun 2020 22:41:59: 1000000 INFO @ Mon, 29 Jun 2020 22:42:01: 7000000 INFO @ Mon, 29 Jun 2020 22:42:04: 2000000 INFO @ Mon, 29 Jun 2020 22:42:06: 8000000 INFO @ Mon, 29 Jun 2020 22:42:09: 3000000 INFO @ Mon, 29 Jun 2020 22:42:11: 9000000 INFO @ Mon, 29 Jun 2020 22:42:14: 4000000 INFO @ Mon, 29 Jun 2020 22:42:16: 10000000 INFO @ Mon, 29 Jun 2020 22:42:19: 5000000 INFO @ Mon, 29 Jun 2020 22:42:21: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:42:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:42:23: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:42:23: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:42:24: 6000000 INFO @ Mon, 29 Jun 2020 22:42:26: 12000000 INFO @ Mon, 29 Jun 2020 22:42:29: 7000000 INFO @ Mon, 29 Jun 2020 22:42:29: 1000000 INFO @ Mon, 29 Jun 2020 22:42:32: 13000000 INFO @ Mon, 29 Jun 2020 22:42:34: 8000000 INFO @ Mon, 29 Jun 2020 22:42:35: 2000000 INFO @ Mon, 29 Jun 2020 22:42:38: 14000000 INFO @ Mon, 29 Jun 2020 22:42:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:42:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:42:39: #1 total tags in treatment: 14196181 INFO @ Mon, 29 Jun 2020 22:42:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:42:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:42:39: 9000000 INFO @ Mon, 29 Jun 2020 22:42:39: #1 tags after filtering in treatment: 14196181 INFO @ Mon, 29 Jun 2020 22:42:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:42:39: #1 finished! INFO @ Mon, 29 Jun 2020 22:42:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:42:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:42:40: #2 number of paired peaks: 45 WARNING @ Mon, 29 Jun 2020 22:42:40: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:42:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:42:40: 3000000 INFO @ Mon, 29 Jun 2020 22:42:44: 10000000 INFO @ Mon, 29 Jun 2020 22:42:45: 4000000 INFO @ Mon, 29 Jun 2020 22:42:49: 11000000 INFO @ Mon, 29 Jun 2020 22:42:50: 5000000 INFO @ Mon, 29 Jun 2020 22:42:54: 12000000 INFO @ Mon, 29 Jun 2020 22:42:56: 6000000 INFO @ Mon, 29 Jun 2020 22:42:59: 13000000 INFO @ Mon, 29 Jun 2020 22:43:01: 7000000 INFO @ Mon, 29 Jun 2020 22:43:05: 14000000 INFO @ Mon, 29 Jun 2020 22:43:06: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:43:06: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:43:06: #1 total tags in treatment: 14196181 INFO @ Mon, 29 Jun 2020 22:43:06: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:43:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:43:06: 8000000 INFO @ Mon, 29 Jun 2020 22:43:06: #1 tags after filtering in treatment: 14196181 INFO @ Mon, 29 Jun 2020 22:43:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:43:06: #1 finished! INFO @ Mon, 29 Jun 2020 22:43:06: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:43:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:43:07: #2 number of paired peaks: 45 WARNING @ Mon, 29 Jun 2020 22:43:07: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:43:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:43:11: 9000000 INFO @ Mon, 29 Jun 2020 22:43:16: 10000000 INFO @ Mon, 29 Jun 2020 22:43:21: 11000000 INFO @ Mon, 29 Jun 2020 22:43:27: 12000000 INFO @ Mon, 29 Jun 2020 22:43:32: 13000000 INFO @ Mon, 29 Jun 2020 22:43:37: 14000000 INFO @ Mon, 29 Jun 2020 22:43:38: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:43:38: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:43:38: #1 total tags in treatment: 14196181 INFO @ Mon, 29 Jun 2020 22:43:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:43:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:43:38: #1 tags after filtering in treatment: 14196181 INFO @ Mon, 29 Jun 2020 22:43:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:43:38: #1 finished! INFO @ Mon, 29 Jun 2020 22:43:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:43:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:43:39: #2 number of paired peaks: 45 WARNING @ Mon, 29 Jun 2020 22:43:39: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:43:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287915/SRX287915.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。