Job ID = 6527862 SRX = SRX287905 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:35:37 prefetch.2.10.7: 1) Downloading 'SRR870094'... 2020-06-29T13:35:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:37:59 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:37:59 prefetch.2.10.7: 1) 'SRR870094' was downloaded successfully Read 18412477 spots for SRR870094/SRR870094.sra Written 18412477 spots for SRR870094/SRR870094.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:02 18412477 reads; of these: 18412477 (100.00%) were unpaired; of these: 986723 (5.36%) aligned 0 times 13569885 (73.70%) aligned exactly 1 time 3855869 (20.94%) aligned >1 times 94.64% overall alignment rate Time searching: 00:06:03 Overall time: 00:06:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1205108 / 17425754 = 0.0692 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:53:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:53:49: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:53:49: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:53:55: 1000000 INFO @ Mon, 29 Jun 2020 22:54:01: 2000000 INFO @ Mon, 29 Jun 2020 22:54:07: 3000000 INFO @ Mon, 29 Jun 2020 22:54:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:54:19: 5000000 INFO @ Mon, 29 Jun 2020 22:54:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:54:19: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:54:19: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:54:25: 6000000 INFO @ Mon, 29 Jun 2020 22:54:25: 1000000 INFO @ Mon, 29 Jun 2020 22:54:32: 7000000 INFO @ Mon, 29 Jun 2020 22:54:32: 2000000 INFO @ Mon, 29 Jun 2020 22:54:38: 8000000 INFO @ Mon, 29 Jun 2020 22:54:38: 3000000 INFO @ Mon, 29 Jun 2020 22:54:44: 9000000 INFO @ Mon, 29 Jun 2020 22:54:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:54:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:54:49: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:54:49: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:54:51: 10000000 INFO @ Mon, 29 Jun 2020 22:54:52: 5000000 INFO @ Mon, 29 Jun 2020 22:54:55: 1000000 INFO @ Mon, 29 Jun 2020 22:54:57: 11000000 INFO @ Mon, 29 Jun 2020 22:54:58: 6000000 INFO @ Mon, 29 Jun 2020 22:55:02: 2000000 INFO @ Mon, 29 Jun 2020 22:55:04: 12000000 INFO @ Mon, 29 Jun 2020 22:55:05: 7000000 INFO @ Mon, 29 Jun 2020 22:55:09: 3000000 INFO @ Mon, 29 Jun 2020 22:55:11: 13000000 INFO @ Mon, 29 Jun 2020 22:55:11: 8000000 INFO @ Mon, 29 Jun 2020 22:55:15: 4000000 INFO @ Mon, 29 Jun 2020 22:55:17: 14000000 INFO @ Mon, 29 Jun 2020 22:55:18: 9000000 INFO @ Mon, 29 Jun 2020 22:55:22: 5000000 INFO @ Mon, 29 Jun 2020 22:55:24: 15000000 INFO @ Mon, 29 Jun 2020 22:55:25: 10000000 INFO @ Mon, 29 Jun 2020 22:55:29: 6000000 INFO @ Mon, 29 Jun 2020 22:55:31: 16000000 INFO @ Mon, 29 Jun 2020 22:55:32: 11000000 INFO @ Mon, 29 Jun 2020 22:55:33: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:55:33: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:55:33: #1 total tags in treatment: 16220646 INFO @ Mon, 29 Jun 2020 22:55:33: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:55:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:55:33: #1 tags after filtering in treatment: 16220646 INFO @ Mon, 29 Jun 2020 22:55:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:55:33: #1 finished! INFO @ Mon, 29 Jun 2020 22:55:33: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:55:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:55:34: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 22:55:34: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:55:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:55:36: 7000000 INFO @ Mon, 29 Jun 2020 22:55:39: 12000000 INFO @ Mon, 29 Jun 2020 22:55:43: 8000000 INFO @ Mon, 29 Jun 2020 22:55:46: 13000000 INFO @ Mon, 29 Jun 2020 22:55:50: 9000000 INFO @ Mon, 29 Jun 2020 22:55:53: 14000000 INFO @ Mon, 29 Jun 2020 22:55:56: 10000000 INFO @ Mon, 29 Jun 2020 22:55:59: 15000000 INFO @ Mon, 29 Jun 2020 22:56:03: 11000000 INFO @ Mon, 29 Jun 2020 22:56:06: 16000000 INFO @ Mon, 29 Jun 2020 22:56:08: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:56:08: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:56:08: #1 total tags in treatment: 16220646 INFO @ Mon, 29 Jun 2020 22:56:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:56:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:56:08: #1 tags after filtering in treatment: 16220646 INFO @ Mon, 29 Jun 2020 22:56:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:56:08: #1 finished! INFO @ Mon, 29 Jun 2020 22:56:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:56:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:56:09: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 22:56:09: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:56:09: Process for pairing-model is terminated! INFO @ Mon, 29 Jun 2020 22:56:10: 12000000 cut: /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:56:16: 13000000 INFO @ Mon, 29 Jun 2020 22:56:22: 14000000 INFO @ Mon, 29 Jun 2020 22:56:28: 15000000 INFO @ Mon, 29 Jun 2020 22:56:34: 16000000 INFO @ Mon, 29 Jun 2020 22:56:35: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:56:35: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:56:35: #1 total tags in treatment: 16220646 INFO @ Mon, 29 Jun 2020 22:56:35: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:56:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:56:35: #1 tags after filtering in treatment: 16220646 INFO @ Mon, 29 Jun 2020 22:56:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:56:35: #1 finished! INFO @ Mon, 29 Jun 2020 22:56:35: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:56:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:56:36: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 22:56:36: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:56:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287905/SRX287905.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。