Job ID = 6527861 SRX = SRX287896 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:19:20 prefetch.2.10.7: 1) Downloading 'SRR870085'... 2020-06-29T13:19:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:22:15 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:22:15 prefetch.2.10.7: 1) 'SRR870085' was downloaded successfully Read 21093334 spots for SRR870085/SRR870085.sra Written 21093334 spots for SRR870085/SRR870085.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:49 21093334 reads; of these: 21093334 (100.00%) were unpaired; of these: 1183164 (5.61%) aligned 0 times 14665029 (69.52%) aligned exactly 1 time 5245141 (24.87%) aligned >1 times 94.39% overall alignment rate Time searching: 00:07:49 Overall time: 00:07:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2193131 / 19910170 = 0.1102 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:44:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:44:09: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:44:09: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:44:14: 1000000 INFO @ Mon, 29 Jun 2020 22:44:19: 2000000 INFO @ Mon, 29 Jun 2020 22:44:24: 3000000 INFO @ Mon, 29 Jun 2020 22:44:29: 4000000 INFO @ Mon, 29 Jun 2020 22:44:34: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:44:39: 6000000 INFO @ Mon, 29 Jun 2020 22:44:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:44:39: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:44:39: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:44:44: 7000000 INFO @ Mon, 29 Jun 2020 22:44:44: 1000000 INFO @ Mon, 29 Jun 2020 22:44:49: 8000000 INFO @ Mon, 29 Jun 2020 22:44:50: 2000000 INFO @ Mon, 29 Jun 2020 22:44:54: 9000000 INFO @ Mon, 29 Jun 2020 22:44:55: 3000000 INFO @ Mon, 29 Jun 2020 22:44:58: 10000000 INFO @ Mon, 29 Jun 2020 22:45:00: 4000000 INFO @ Mon, 29 Jun 2020 22:45:03: 11000000 INFO @ Mon, 29 Jun 2020 22:45:05: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:45:08: 12000000 INFO @ Mon, 29 Jun 2020 22:45:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:45:09: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:45:09: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:45:11: 6000000 INFO @ Mon, 29 Jun 2020 22:45:14: 13000000 INFO @ Mon, 29 Jun 2020 22:45:15: 1000000 INFO @ Mon, 29 Jun 2020 22:45:16: 7000000 INFO @ Mon, 29 Jun 2020 22:45:19: 14000000 INFO @ Mon, 29 Jun 2020 22:45:20: 2000000 INFO @ Mon, 29 Jun 2020 22:45:21: 8000000 INFO @ Mon, 29 Jun 2020 22:45:24: 15000000 INFO @ Mon, 29 Jun 2020 22:45:26: 3000000 INFO @ Mon, 29 Jun 2020 22:45:26: 9000000 INFO @ Mon, 29 Jun 2020 22:45:30: 16000000 INFO @ Mon, 29 Jun 2020 22:45:31: 4000000 INFO @ Mon, 29 Jun 2020 22:45:31: 10000000 INFO @ Mon, 29 Jun 2020 22:45:35: 17000000 INFO @ Mon, 29 Jun 2020 22:45:36: 5000000 INFO @ Mon, 29 Jun 2020 22:45:36: 11000000 INFO @ Mon, 29 Jun 2020 22:45:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:45:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:45:39: #1 total tags in treatment: 17717039 INFO @ Mon, 29 Jun 2020 22:45:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:45:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:45:39: #1 tags after filtering in treatment: 17717039 INFO @ Mon, 29 Jun 2020 22:45:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:45:39: #1 finished! INFO @ Mon, 29 Jun 2020 22:45:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:45:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:45:40: #2 number of paired peaks: 57 WARNING @ Mon, 29 Jun 2020 22:45:40: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:45:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:45:41: 6000000 INFO @ Mon, 29 Jun 2020 22:45:41: 12000000 INFO @ Mon, 29 Jun 2020 22:45:46: 7000000 INFO @ Mon, 29 Jun 2020 22:45:47: 13000000 INFO @ Mon, 29 Jun 2020 22:45:51: 8000000 INFO @ Mon, 29 Jun 2020 22:45:52: 14000000 INFO @ Mon, 29 Jun 2020 22:45:56: 9000000 INFO @ Mon, 29 Jun 2020 22:45:57: 15000000 INFO @ Mon, 29 Jun 2020 22:46:01: 10000000 INFO @ Mon, 29 Jun 2020 22:46:02: 16000000 INFO @ Mon, 29 Jun 2020 22:46:06: 11000000 INFO @ Mon, 29 Jun 2020 22:46:07: 17000000 INFO @ Mon, 29 Jun 2020 22:46:11: 12000000 INFO @ Mon, 29 Jun 2020 22:46:11: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:46:11: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:46:11: #1 total tags in treatment: 17717039 INFO @ Mon, 29 Jun 2020 22:46:11: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:46:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:46:12: #1 tags after filtering in treatment: 17717039 INFO @ Mon, 29 Jun 2020 22:46:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:46:12: #1 finished! INFO @ Mon, 29 Jun 2020 22:46:12: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:46:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:46:13: #2 number of paired peaks: 57 WARNING @ Mon, 29 Jun 2020 22:46:13: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:46:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:46:16: 13000000 INFO @ Mon, 29 Jun 2020 22:46:21: 14000000 INFO @ Mon, 29 Jun 2020 22:46:26: 15000000 INFO @ Mon, 29 Jun 2020 22:46:30: 16000000 INFO @ Mon, 29 Jun 2020 22:46:35: 17000000 INFO @ Mon, 29 Jun 2020 22:46:39: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:46:39: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:46:39: #1 total tags in treatment: 17717039 INFO @ Mon, 29 Jun 2020 22:46:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:46:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:46:39: #1 tags after filtering in treatment: 17717039 INFO @ Mon, 29 Jun 2020 22:46:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:46:39: #1 finished! INFO @ Mon, 29 Jun 2020 22:46:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:46:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:46:40: #2 number of paired peaks: 57 WARNING @ Mon, 29 Jun 2020 22:46:40: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:46:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287896/SRX287896.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。