Job ID = 6527850 SRX = SRX287873 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:49:14 prefetch.2.10.7: 1) Downloading 'SRR870062'... 2020-06-29T13:49:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:51:20 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:51:20 prefetch.2.10.7: 1) 'SRR870062' was downloaded successfully Read 17761171 spots for SRR870062/SRR870062.sra Written 17761171 spots for SRR870062/SRR870062.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:20 17761171 reads; of these: 17761171 (100.00%) were unpaired; of these: 964244 (5.43%) aligned 0 times 12931007 (72.80%) aligned exactly 1 time 3865920 (21.77%) aligned >1 times 94.57% overall alignment rate Time searching: 00:05:20 Overall time: 00:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1267621 / 16796927 = 0.0755 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:58: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:58: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:03: 1000000 INFO @ Mon, 29 Jun 2020 23:06:08: 2000000 INFO @ Mon, 29 Jun 2020 23:06:13: 3000000 INFO @ Mon, 29 Jun 2020 23:06:18: 4000000 INFO @ Mon, 29 Jun 2020 23:06:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:27: 6000000 INFO @ Mon, 29 Jun 2020 23:06:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:28: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:28: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:32: 7000000 INFO @ Mon, 29 Jun 2020 23:06:34: 1000000 INFO @ Mon, 29 Jun 2020 23:06:37: 8000000 INFO @ Mon, 29 Jun 2020 23:06:40: 2000000 INFO @ Mon, 29 Jun 2020 23:06:42: 9000000 INFO @ Mon, 29 Jun 2020 23:06:45: 3000000 INFO @ Mon, 29 Jun 2020 23:06:47: 10000000 INFO @ Mon, 29 Jun 2020 23:06:51: 4000000 INFO @ Mon, 29 Jun 2020 23:06:52: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:57: 5000000 INFO @ Mon, 29 Jun 2020 23:06:57: 12000000 INFO @ Mon, 29 Jun 2020 23:06:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:58: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:58: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:07:02: 13000000 INFO @ Mon, 29 Jun 2020 23:07:03: 6000000 INFO @ Mon, 29 Jun 2020 23:07:03: 1000000 INFO @ Mon, 29 Jun 2020 23:07:07: 14000000 INFO @ Mon, 29 Jun 2020 23:07:08: 7000000 INFO @ Mon, 29 Jun 2020 23:07:08: 2000000 INFO @ Mon, 29 Jun 2020 23:07:13: 15000000 INFO @ Mon, 29 Jun 2020 23:07:13: 3000000 INFO @ Mon, 29 Jun 2020 23:07:14: 8000000 INFO @ Mon, 29 Jun 2020 23:07:15: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:15: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:15: #1 total tags in treatment: 15529306 INFO @ Mon, 29 Jun 2020 23:07:15: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:16: #1 tags after filtering in treatment: 15529306 INFO @ Mon, 29 Jun 2020 23:07:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:16: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:17: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 23:07:17: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:07:18: 4000000 INFO @ Mon, 29 Jun 2020 23:07:20: 9000000 INFO @ Mon, 29 Jun 2020 23:07:23: 5000000 INFO @ Mon, 29 Jun 2020 23:07:25: 10000000 INFO @ Mon, 29 Jun 2020 23:07:28: 6000000 INFO @ Mon, 29 Jun 2020 23:07:31: 11000000 INFO @ Mon, 29 Jun 2020 23:07:33: 7000000 INFO @ Mon, 29 Jun 2020 23:07:37: 12000000 INFO @ Mon, 29 Jun 2020 23:07:38: 8000000 INFO @ Mon, 29 Jun 2020 23:07:43: 13000000 INFO @ Mon, 29 Jun 2020 23:07:43: 9000000 INFO @ Mon, 29 Jun 2020 23:07:48: 10000000 INFO @ Mon, 29 Jun 2020 23:07:49: 14000000 INFO @ Mon, 29 Jun 2020 23:07:53: 11000000 INFO @ Mon, 29 Jun 2020 23:07:54: 15000000 INFO @ Mon, 29 Jun 2020 23:07:57: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:57: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:57: #1 total tags in treatment: 15529306 INFO @ Mon, 29 Jun 2020 23:07:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:58: #1 tags after filtering in treatment: 15529306 INFO @ Mon, 29 Jun 2020 23:07:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:58: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:58: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:58: 12000000 INFO @ Mon, 29 Jun 2020 23:07:59: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 23:07:59: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:08:03: 13000000 INFO @ Mon, 29 Jun 2020 23:08:08: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:08:13: 15000000 INFO @ Mon, 29 Jun 2020 23:08:16: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:16: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:16: #1 total tags in treatment: 15529306 INFO @ Mon, 29 Jun 2020 23:08:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:16: #1 tags after filtering in treatment: 15529306 INFO @ Mon, 29 Jun 2020 23:08:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:16: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:17: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 23:08:17: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287873/SRX287873.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。