Job ID = 6527847 SRX = SRX287860 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:05:21 prefetch.2.10.7: 1) Downloading 'SRR870049'... 2020-06-29T14:05:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:09:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:09:04 prefetch.2.10.7: 1) 'SRR870049' was downloaded successfully Read 18754483 spots for SRR870049/SRR870049.sra Written 18754483 spots for SRR870049/SRR870049.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:43 18754483 reads; of these: 18754483 (100.00%) were unpaired; of these: 1011721 (5.39%) aligned 0 times 13217543 (70.48%) aligned exactly 1 time 4525219 (24.13%) aligned >1 times 94.61% overall alignment rate Time searching: 00:05:43 Overall time: 00:05:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1418439 / 17742762 = 0.0799 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:24:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:24:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:24:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:24:42: 1000000 INFO @ Mon, 29 Jun 2020 23:24:50: 2000000 INFO @ Mon, 29 Jun 2020 23:24:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:25:05: 4000000 INFO @ Mon, 29 Jun 2020 23:25:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:25:05: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:25:05: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:25:13: 5000000 INFO @ Mon, 29 Jun 2020 23:25:13: 1000000 INFO @ Mon, 29 Jun 2020 23:25:21: 6000000 INFO @ Mon, 29 Jun 2020 23:25:21: 2000000 INFO @ Mon, 29 Jun 2020 23:25:29: 7000000 INFO @ Mon, 29 Jun 2020 23:25:29: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:25:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:25:35: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:25:35: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:25:38: 8000000 INFO @ Mon, 29 Jun 2020 23:25:38: 4000000 INFO @ Mon, 29 Jun 2020 23:25:43: 1000000 INFO @ Mon, 29 Jun 2020 23:25:46: 9000000 INFO @ Mon, 29 Jun 2020 23:25:46: 5000000 INFO @ Mon, 29 Jun 2020 23:25:51: 2000000 INFO @ Mon, 29 Jun 2020 23:25:55: 10000000 INFO @ Mon, 29 Jun 2020 23:25:55: 6000000 INFO @ Mon, 29 Jun 2020 23:25:59: 3000000 INFO @ Mon, 29 Jun 2020 23:26:04: 11000000 INFO @ Mon, 29 Jun 2020 23:26:04: 7000000 INFO @ Mon, 29 Jun 2020 23:26:07: 4000000 INFO @ Mon, 29 Jun 2020 23:26:12: 12000000 INFO @ Mon, 29 Jun 2020 23:26:12: 8000000 INFO @ Mon, 29 Jun 2020 23:26:15: 5000000 INFO @ Mon, 29 Jun 2020 23:26:21: 9000000 INFO @ Mon, 29 Jun 2020 23:26:21: 13000000 INFO @ Mon, 29 Jun 2020 23:26:24: 6000000 INFO @ Mon, 29 Jun 2020 23:26:30: 10000000 INFO @ Mon, 29 Jun 2020 23:26:30: 14000000 INFO @ Mon, 29 Jun 2020 23:26:32: 7000000 INFO @ Mon, 29 Jun 2020 23:26:38: 11000000 INFO @ Mon, 29 Jun 2020 23:26:39: 15000000 INFO @ Mon, 29 Jun 2020 23:26:40: 8000000 INFO @ Mon, 29 Jun 2020 23:26:47: 12000000 INFO @ Mon, 29 Jun 2020 23:26:47: 16000000 INFO @ Mon, 29 Jun 2020 23:26:48: 9000000 INFO @ Mon, 29 Jun 2020 23:26:50: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:26:50: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:26:50: #1 total tags in treatment: 16324323 INFO @ Mon, 29 Jun 2020 23:26:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:26:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:26:50: #1 tags after filtering in treatment: 16324323 INFO @ Mon, 29 Jun 2020 23:26:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:26:50: #1 finished! INFO @ Mon, 29 Jun 2020 23:26:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:26:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:26:51: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:26:51: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:26:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:26:56: 13000000 INFO @ Mon, 29 Jun 2020 23:26:56: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:27:04: 11000000 INFO @ Mon, 29 Jun 2020 23:27:04: 14000000 INFO @ Mon, 29 Jun 2020 23:27:12: 12000000 INFO @ Mon, 29 Jun 2020 23:27:12: 15000000 INFO @ Mon, 29 Jun 2020 23:27:20: 13000000 INFO @ Mon, 29 Jun 2020 23:27:21: 16000000 INFO @ Mon, 29 Jun 2020 23:27:23: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:27:23: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:27:23: #1 total tags in treatment: 16324323 INFO @ Mon, 29 Jun 2020 23:27:23: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:27:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:27:23: #1 tags after filtering in treatment: 16324323 INFO @ Mon, 29 Jun 2020 23:27:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:27:23: #1 finished! INFO @ Mon, 29 Jun 2020 23:27:23: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:27:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:27:24: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:27:24: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:27:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:27:28: 14000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:27:35: 15000000 INFO @ Mon, 29 Jun 2020 23:27:42: 16000000 INFO @ Mon, 29 Jun 2020 23:27:45: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:27:45: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:27:45: #1 total tags in treatment: 16324323 INFO @ Mon, 29 Jun 2020 23:27:45: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:27:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:27:45: #1 tags after filtering in treatment: 16324323 INFO @ Mon, 29 Jun 2020 23:27:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:27:45: #1 finished! INFO @ Mon, 29 Jun 2020 23:27:45: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:27:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:27:46: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:27:46: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:27:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287860/SRX287860.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling