Job ID = 6527844 SRX = SRX287852 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:00:06 prefetch.2.10.7: 1) Downloading 'SRR870041'... 2020-06-29T14:00:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:03:44 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:03:44 prefetch.2.10.7: 1) 'SRR870041' was downloaded successfully Read 18437679 spots for SRR870041/SRR870041.sra Written 18437679 spots for SRR870041/SRR870041.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:57 18437679 reads; of these: 18437679 (100.00%) were unpaired; of these: 1269750 (6.89%) aligned 0 times 14840712 (80.49%) aligned exactly 1 time 2327217 (12.62%) aligned >1 times 93.11% overall alignment rate Time searching: 00:04:58 Overall time: 00:04:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1616482 / 17167929 = 0.0942 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:19:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:19:20: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:19:20: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:19:27: 1000000 INFO @ Mon, 29 Jun 2020 23:19:34: 2000000 INFO @ Mon, 29 Jun 2020 23:19:41: 3000000 INFO @ Mon, 29 Jun 2020 23:19:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:19:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:19:50: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:19:50: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:19:54: 5000000 INFO @ Mon, 29 Jun 2020 23:19:58: 1000000 INFO @ Mon, 29 Jun 2020 23:20:02: 6000000 INFO @ Mon, 29 Jun 2020 23:20:05: 2000000 INFO @ Mon, 29 Jun 2020 23:20:09: 7000000 INFO @ Mon, 29 Jun 2020 23:20:12: 3000000 INFO @ Mon, 29 Jun 2020 23:20:16: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:20:19: 4000000 INFO @ Mon, 29 Jun 2020 23:20:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:20:20: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:20:20: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:20:24: 9000000 INFO @ Mon, 29 Jun 2020 23:20:26: 5000000 INFO @ Mon, 29 Jun 2020 23:20:28: 1000000 INFO @ Mon, 29 Jun 2020 23:20:31: 10000000 INFO @ Mon, 29 Jun 2020 23:20:34: 6000000 INFO @ Mon, 29 Jun 2020 23:20:35: 2000000 INFO @ Mon, 29 Jun 2020 23:20:38: 11000000 INFO @ Mon, 29 Jun 2020 23:20:41: 7000000 INFO @ Mon, 29 Jun 2020 23:20:43: 3000000 INFO @ Mon, 29 Jun 2020 23:20:46: 12000000 INFO @ Mon, 29 Jun 2020 23:20:49: 8000000 INFO @ Mon, 29 Jun 2020 23:20:50: 4000000 INFO @ Mon, 29 Jun 2020 23:20:53: 13000000 INFO @ Mon, 29 Jun 2020 23:20:56: 9000000 INFO @ Mon, 29 Jun 2020 23:20:58: 5000000 INFO @ Mon, 29 Jun 2020 23:21:00: 14000000 INFO @ Mon, 29 Jun 2020 23:21:03: 10000000 INFO @ Mon, 29 Jun 2020 23:21:06: 6000000 INFO @ Mon, 29 Jun 2020 23:21:08: 15000000 INFO @ Mon, 29 Jun 2020 23:21:11: 11000000 INFO @ Mon, 29 Jun 2020 23:21:12: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:21:12: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:21:12: #1 total tags in treatment: 15551447 INFO @ Mon, 29 Jun 2020 23:21:12: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:21:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:21:12: #1 tags after filtering in treatment: 15551447 INFO @ Mon, 29 Jun 2020 23:21:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:21:12: #1 finished! INFO @ Mon, 29 Jun 2020 23:21:12: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:21:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:21:13: #2 number of paired peaks: 9 WARNING @ Mon, 29 Jun 2020 23:21:13: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:21:13: Process for pairing-model is terminated! INFO @ Mon, 29 Jun 2020 23:21:13: 7000000 cut: /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:21:18: 12000000 INFO @ Mon, 29 Jun 2020 23:21:20: 8000000 INFO @ Mon, 29 Jun 2020 23:21:25: 13000000 INFO @ Mon, 29 Jun 2020 23:21:28: 9000000 INFO @ Mon, 29 Jun 2020 23:21:32: 14000000 INFO @ Mon, 29 Jun 2020 23:21:35: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:21:39: 15000000 INFO @ Mon, 29 Jun 2020 23:21:42: 11000000 INFO @ Mon, 29 Jun 2020 23:21:43: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:21:43: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:21:43: #1 total tags in treatment: 15551447 INFO @ Mon, 29 Jun 2020 23:21:43: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:21:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:21:43: #1 tags after filtering in treatment: 15551447 INFO @ Mon, 29 Jun 2020 23:21:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:21:43: #1 finished! INFO @ Mon, 29 Jun 2020 23:21:43: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:21:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:21:45: #2 number of paired peaks: 9 WARNING @ Mon, 29 Jun 2020 23:21:45: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:21:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:21:49: 12000000 INFO @ Mon, 29 Jun 2020 23:21:56: 13000000 INFO @ Mon, 29 Jun 2020 23:22:03: 14000000 INFO @ Mon, 29 Jun 2020 23:22:09: 15000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:22:13: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:22:13: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:22:13: #1 total tags in treatment: 15551447 INFO @ Mon, 29 Jun 2020 23:22:13: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:22:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:22:13: #1 tags after filtering in treatment: 15551447 INFO @ Mon, 29 Jun 2020 23:22:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:22:13: #1 finished! INFO @ Mon, 29 Jun 2020 23:22:13: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:22:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:22:14: #2 number of paired peaks: 9 WARNING @ Mon, 29 Jun 2020 23:22:14: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:22:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287852/SRX287852.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling