Job ID = 6527843 SRX = SRX287850 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:46:26 prefetch.2.10.7: 1) Downloading 'SRR870039'... 2020-06-29T13:46:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:50:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:50:05 prefetch.2.10.7: 1) 'SRR870039' was downloaded successfully Read 18463214 spots for SRR870039/SRR870039.sra Written 18463214 spots for SRR870039/SRR870039.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:43 18463214 reads; of these: 18463214 (100.00%) were unpaired; of these: 1216704 (6.59%) aligned 0 times 13413022 (72.65%) aligned exactly 1 time 3833488 (20.76%) aligned >1 times 93.41% overall alignment rate Time searching: 00:05:44 Overall time: 00:05:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1194259 / 17246510 = 0.0692 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:05:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:05:48: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:05:48: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:05:54: 1000000 INFO @ Mon, 29 Jun 2020 23:05:59: 2000000 INFO @ Mon, 29 Jun 2020 23:06:05: 3000000 INFO @ Mon, 29 Jun 2020 23:06:10: 4000000 INFO @ Mon, 29 Jun 2020 23:06:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:18: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:18: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:21: 6000000 INFO @ Mon, 29 Jun 2020 23:06:24: 1000000 INFO @ Mon, 29 Jun 2020 23:06:27: 7000000 INFO @ Mon, 29 Jun 2020 23:06:31: 2000000 INFO @ Mon, 29 Jun 2020 23:06:33: 8000000 INFO @ Mon, 29 Jun 2020 23:06:37: 3000000 INFO @ Mon, 29 Jun 2020 23:06:39: 9000000 INFO @ Mon, 29 Jun 2020 23:06:43: 4000000 INFO @ Mon, 29 Jun 2020 23:06:45: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:06:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:06:48: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:06:48: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:06:49: 5000000 INFO @ Mon, 29 Jun 2020 23:06:52: 11000000 INFO @ Mon, 29 Jun 2020 23:06:56: 6000000 INFO @ Mon, 29 Jun 2020 23:06:56: 1000000 INFO @ Mon, 29 Jun 2020 23:06:58: 12000000 INFO @ Mon, 29 Jun 2020 23:07:02: 7000000 INFO @ Mon, 29 Jun 2020 23:07:03: 2000000 INFO @ Mon, 29 Jun 2020 23:07:05: 13000000 INFO @ Mon, 29 Jun 2020 23:07:09: 8000000 INFO @ Mon, 29 Jun 2020 23:07:10: 3000000 INFO @ Mon, 29 Jun 2020 23:07:12: 14000000 INFO @ Mon, 29 Jun 2020 23:07:16: 9000000 INFO @ Mon, 29 Jun 2020 23:07:17: 4000000 INFO @ Mon, 29 Jun 2020 23:07:18: 15000000 INFO @ Mon, 29 Jun 2020 23:07:22: 10000000 INFO @ Mon, 29 Jun 2020 23:07:24: 5000000 INFO @ Mon, 29 Jun 2020 23:07:25: 16000000 INFO @ Mon, 29 Jun 2020 23:07:25: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:07:25: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:07:25: #1 total tags in treatment: 16052251 INFO @ Mon, 29 Jun 2020 23:07:25: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:07:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:07:26: #1 tags after filtering in treatment: 16052251 INFO @ Mon, 29 Jun 2020 23:07:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:07:26: #1 finished! INFO @ Mon, 29 Jun 2020 23:07:26: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:07:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:07:27: #2 number of paired peaks: 65 WARNING @ Mon, 29 Jun 2020 23:07:27: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:07:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:07:29: 11000000 INFO @ Mon, 29 Jun 2020 23:07:30: 6000000 INFO @ Mon, 29 Jun 2020 23:07:35: 12000000 INFO @ Mon, 29 Jun 2020 23:07:37: 7000000 INFO @ Mon, 29 Jun 2020 23:07:42: 13000000 INFO @ Mon, 29 Jun 2020 23:07:43: 8000000 INFO @ Mon, 29 Jun 2020 23:07:48: 14000000 INFO @ Mon, 29 Jun 2020 23:07:50: 9000000 INFO @ Mon, 29 Jun 2020 23:07:54: 15000000 INFO @ Mon, 29 Jun 2020 23:07:56: 10000000 INFO @ Mon, 29 Jun 2020 23:08:01: 16000000 INFO @ Mon, 29 Jun 2020 23:08:01: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:01: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:01: #1 total tags in treatment: 16052251 INFO @ Mon, 29 Jun 2020 23:08:01: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:08:02: #1 tags after filtering in treatment: 16052251 INFO @ Mon, 29 Jun 2020 23:08:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:02: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:02: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:02: 11000000 INFO @ Mon, 29 Jun 2020 23:08:03: #2 number of paired peaks: 65 WARNING @ Mon, 29 Jun 2020 23:08:03: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:08:08: 12000000 INFO @ Mon, 29 Jun 2020 23:08:15: 13000000 INFO @ Mon, 29 Jun 2020 23:08:20: 14000000 INFO @ Mon, 29 Jun 2020 23:08:26: 15000000 INFO @ Mon, 29 Jun 2020 23:08:32: 16000000 INFO @ Mon, 29 Jun 2020 23:08:32: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:08:32: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:08:32: #1 total tags in treatment: 16052251 INFO @ Mon, 29 Jun 2020 23:08:32: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:08:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:08:32: #1 tags after filtering in treatment: 16052251 INFO @ Mon, 29 Jun 2020 23:08:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:08:32: #1 finished! INFO @ Mon, 29 Jun 2020 23:08:32: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:08:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:08:33: #2 number of paired peaks: 65 WARNING @ Mon, 29 Jun 2020 23:08:33: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:08:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 14 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287850/SRX287850.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。