Job ID = 6527841 SRX = SRX287846 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:59:21 prefetch.2.10.7: 1) Downloading 'SRR870035'... 2020-06-29T13:59:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:04:31 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:04:31 prefetch.2.10.7: 1) 'SRR870035' was downloaded successfully Read 21014950 spots for SRR870035/SRR870035.sra Written 21014950 spots for SRR870035/SRR870035.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:22 21014950 reads; of these: 21014950 (100.00%) were unpaired; of these: 1185196 (5.64%) aligned 0 times 14968794 (71.23%) aligned exactly 1 time 4860960 (23.13%) aligned >1 times 94.36% overall alignment rate Time searching: 00:07:23 Overall time: 00:07:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2682781 / 19829754 = 0.1353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:22:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:22:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:22:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:22:37: 1000000 INFO @ Mon, 29 Jun 2020 23:22:44: 2000000 INFO @ Mon, 29 Jun 2020 23:22:50: 3000000 INFO @ Mon, 29 Jun 2020 23:22:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:23:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:23:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:23:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:23:04: 5000000 INFO @ Mon, 29 Jun 2020 23:23:08: 1000000 INFO @ Mon, 29 Jun 2020 23:23:11: 6000000 INFO @ Mon, 29 Jun 2020 23:23:15: 2000000 INFO @ Mon, 29 Jun 2020 23:23:18: 7000000 INFO @ Mon, 29 Jun 2020 23:23:22: 3000000 INFO @ Mon, 29 Jun 2020 23:23:24: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:23:29: 4000000 INFO @ Mon, 29 Jun 2020 23:23:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:23:30: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:23:30: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:23:31: 9000000 INFO @ Mon, 29 Jun 2020 23:23:36: 5000000 INFO @ Mon, 29 Jun 2020 23:23:37: 1000000 INFO @ Mon, 29 Jun 2020 23:23:38: 10000000 INFO @ Mon, 29 Jun 2020 23:23:43: 6000000 INFO @ Mon, 29 Jun 2020 23:23:43: 2000000 INFO @ Mon, 29 Jun 2020 23:23:45: 11000000 INFO @ Mon, 29 Jun 2020 23:23:50: 3000000 INFO @ Mon, 29 Jun 2020 23:23:50: 7000000 INFO @ Mon, 29 Jun 2020 23:23:52: 12000000 INFO @ Mon, 29 Jun 2020 23:23:56: 4000000 INFO @ Mon, 29 Jun 2020 23:23:57: 8000000 INFO @ Mon, 29 Jun 2020 23:23:59: 13000000 INFO @ Mon, 29 Jun 2020 23:24:03: 5000000 INFO @ Mon, 29 Jun 2020 23:24:04: 9000000 INFO @ Mon, 29 Jun 2020 23:24:06: 14000000 INFO @ Mon, 29 Jun 2020 23:24:09: 6000000 INFO @ Mon, 29 Jun 2020 23:24:11: 10000000 INFO @ Mon, 29 Jun 2020 23:24:13: 15000000 INFO @ Mon, 29 Jun 2020 23:24:16: 7000000 INFO @ Mon, 29 Jun 2020 23:24:18: 11000000 INFO @ Mon, 29 Jun 2020 23:24:19: 16000000 INFO @ Mon, 29 Jun 2020 23:24:22: 8000000 INFO @ Mon, 29 Jun 2020 23:24:25: 12000000 INFO @ Mon, 29 Jun 2020 23:24:26: 17000000 INFO @ Mon, 29 Jun 2020 23:24:27: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:24:27: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:24:27: #1 total tags in treatment: 17146973 INFO @ Mon, 29 Jun 2020 23:24:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:24:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:24:28: #1 tags after filtering in treatment: 17146973 INFO @ Mon, 29 Jun 2020 23:24:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:24:28: #1 finished! INFO @ Mon, 29 Jun 2020 23:24:28: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:24:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:24:28: 9000000 INFO @ Mon, 29 Jun 2020 23:24:29: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 23:24:29: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:24:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:24:32: 13000000 INFO @ Mon, 29 Jun 2020 23:24:35: 10000000 INFO @ Mon, 29 Jun 2020 23:24:39: 14000000 INFO @ Mon, 29 Jun 2020 23:24:41: 11000000 INFO @ Mon, 29 Jun 2020 23:24:46: 15000000 INFO @ Mon, 29 Jun 2020 23:24:47: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:24:53: 16000000 INFO @ Mon, 29 Jun 2020 23:24:54: 13000000 INFO @ Mon, 29 Jun 2020 23:25:00: 17000000 INFO @ Mon, 29 Jun 2020 23:25:00: 14000000 INFO @ Mon, 29 Jun 2020 23:25:01: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:25:01: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:25:01: #1 total tags in treatment: 17146973 INFO @ Mon, 29 Jun 2020 23:25:01: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:25:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:25:01: #1 tags after filtering in treatment: 17146973 INFO @ Mon, 29 Jun 2020 23:25:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:25:01: #1 finished! INFO @ Mon, 29 Jun 2020 23:25:01: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:25:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:25:02: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 23:25:02: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:25:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:25:06: 15000000 INFO @ Mon, 29 Jun 2020 23:25:12: 16000000 INFO @ Mon, 29 Jun 2020 23:25:18: 17000000 INFO @ Mon, 29 Jun 2020 23:25:19: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:25:19: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:25:19: #1 total tags in treatment: 17146973 INFO @ Mon, 29 Jun 2020 23:25:19: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:25:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:25:19: #1 tags after filtering in treatment: 17146973 INFO @ Mon, 29 Jun 2020 23:25:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:25:19: #1 finished! INFO @ Mon, 29 Jun 2020 23:25:19: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:25:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:25:20: #2 number of paired peaks: 68 WARNING @ Mon, 29 Jun 2020 23:25:20: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:25:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287846/SRX287846.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。