Job ID = 6527840 SRX = SRX287845 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:29:44 prefetch.2.10.7: 1) Downloading 'SRR870034'... 2020-06-29T13:29:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:33:10 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:33:10 prefetch.2.10.7: 1) 'SRR870034' was downloaded successfully Read 17761171 spots for SRR870034/SRR870034.sra Written 17761171 spots for SRR870034/SRR870034.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:43 17761171 reads; of these: 17761171 (100.00%) were unpaired; of these: 964251 (5.43%) aligned 0 times 12930882 (72.80%) aligned exactly 1 time 3866038 (21.77%) aligned >1 times 94.57% overall alignment rate Time searching: 00:05:43 Overall time: 00:05:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1267979 / 16796920 = 0.0755 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:48:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:48:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:48:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:48:58: 1000000 INFO @ Mon, 29 Jun 2020 22:49:03: 2000000 INFO @ Mon, 29 Jun 2020 22:49:08: 3000000 INFO @ Mon, 29 Jun 2020 22:49:12: 4000000 INFO @ Mon, 29 Jun 2020 22:49:17: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:49:22: 6000000 INFO @ Mon, 29 Jun 2020 22:49:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:49:23: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:49:23: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:49:27: 7000000 INFO @ Mon, 29 Jun 2020 22:49:28: 1000000 INFO @ Mon, 29 Jun 2020 22:49:32: 8000000 INFO @ Mon, 29 Jun 2020 22:49:33: 2000000 INFO @ Mon, 29 Jun 2020 22:49:37: 9000000 INFO @ Mon, 29 Jun 2020 22:49:39: 3000000 INFO @ Mon, 29 Jun 2020 22:49:42: 10000000 INFO @ Mon, 29 Jun 2020 22:49:44: 4000000 INFO @ Mon, 29 Jun 2020 22:49:48: 11000000 INFO @ Mon, 29 Jun 2020 22:49:49: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:49:53: 12000000 INFO @ Mon, 29 Jun 2020 22:49:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:49:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:49:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:49:54: 6000000 INFO @ Mon, 29 Jun 2020 22:49:58: 13000000 INFO @ Mon, 29 Jun 2020 22:49:59: 1000000 INFO @ Mon, 29 Jun 2020 22:49:59: 7000000 INFO @ Mon, 29 Jun 2020 22:50:03: 14000000 INFO @ Mon, 29 Jun 2020 22:50:04: 8000000 INFO @ Mon, 29 Jun 2020 22:50:05: 2000000 INFO @ Mon, 29 Jun 2020 22:50:08: 15000000 INFO @ Mon, 29 Jun 2020 22:50:09: 9000000 INFO @ Mon, 29 Jun 2020 22:50:10: 3000000 INFO @ Mon, 29 Jun 2020 22:50:11: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:50:11: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:50:11: #1 total tags in treatment: 15528941 INFO @ Mon, 29 Jun 2020 22:50:11: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:50:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:50:11: #1 tags after filtering in treatment: 15528941 INFO @ Mon, 29 Jun 2020 22:50:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:50:11: #1 finished! INFO @ Mon, 29 Jun 2020 22:50:11: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:50:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:50:12: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 22:50:12: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:50:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:50:15: 10000000 INFO @ Mon, 29 Jun 2020 22:50:16: 4000000 INFO @ Mon, 29 Jun 2020 22:50:20: 11000000 INFO @ Mon, 29 Jun 2020 22:50:22: 5000000 INFO @ Mon, 29 Jun 2020 22:50:25: 12000000 INFO @ Mon, 29 Jun 2020 22:50:28: 6000000 INFO @ Mon, 29 Jun 2020 22:50:30: 13000000 INFO @ Mon, 29 Jun 2020 22:50:34: 7000000 INFO @ Mon, 29 Jun 2020 22:50:35: 14000000 INFO @ Mon, 29 Jun 2020 22:50:40: 8000000 INFO @ Mon, 29 Jun 2020 22:50:40: 15000000 INFO @ Mon, 29 Jun 2020 22:50:43: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:50:43: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:50:43: #1 total tags in treatment: 15528941 INFO @ Mon, 29 Jun 2020 22:50:43: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:50:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:50:43: #1 tags after filtering in treatment: 15528941 INFO @ Mon, 29 Jun 2020 22:50:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:50:43: #1 finished! INFO @ Mon, 29 Jun 2020 22:50:43: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:50:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:50:44: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 22:50:44: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:50:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:50:46: 9000000 INFO @ Mon, 29 Jun 2020 22:50:51: 10000000 INFO @ Mon, 29 Jun 2020 22:50:57: 11000000 INFO @ Mon, 29 Jun 2020 22:51:03: 12000000 INFO @ Mon, 29 Jun 2020 22:51:09: 13000000 INFO @ Mon, 29 Jun 2020 22:51:15: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:51:21: 15000000 INFO @ Mon, 29 Jun 2020 22:51:24: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:51:24: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:51:24: #1 total tags in treatment: 15528941 INFO @ Mon, 29 Jun 2020 22:51:24: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:51:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:51:24: #1 tags after filtering in treatment: 15528941 INFO @ Mon, 29 Jun 2020 22:51:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:51:24: #1 finished! INFO @ Mon, 29 Jun 2020 22:51:24: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:51:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:51:25: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 22:51:25: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:51:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287845/SRX287845.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。