Job ID = 6527838 SRX = SRX287827 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:29:41 prefetch.2.10.7: 1) Downloading 'SRR870016'... 2020-06-29T13:29:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:31:33 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:31:34 prefetch.2.10.7: 'SRR870016' is valid 2020-06-29T13:31:34 prefetch.2.10.7: 1) 'SRR870016' was downloaded successfully Read 13618952 spots for SRR870016/SRR870016.sra Written 13618952 spots for SRR870016/SRR870016.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:25 13618952 reads; of these: 13618952 (100.00%) were unpaired; of these: 674102 (4.95%) aligned 0 times 11613850 (85.28%) aligned exactly 1 time 1331000 (9.77%) aligned >1 times 95.05% overall alignment rate Time searching: 00:03:25 Overall time: 00:03:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 720237 / 12944850 = 0.0556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:42:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:42:34: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:42:34: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:42:39: 1000000 INFO @ Mon, 29 Jun 2020 22:42:45: 2000000 INFO @ Mon, 29 Jun 2020 22:42:50: 3000000 INFO @ Mon, 29 Jun 2020 22:42:55: 4000000 INFO @ Mon, 29 Jun 2020 22:43:01: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:43:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:43:04: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:43:04: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:43:06: 6000000 INFO @ Mon, 29 Jun 2020 22:43:10: 1000000 INFO @ Mon, 29 Jun 2020 22:43:12: 7000000 INFO @ Mon, 29 Jun 2020 22:43:17: 2000000 INFO @ Mon, 29 Jun 2020 22:43:18: 8000000 INFO @ Mon, 29 Jun 2020 22:43:24: 3000000 INFO @ Mon, 29 Jun 2020 22:43:24: 9000000 INFO @ Mon, 29 Jun 2020 22:43:30: 10000000 INFO @ Mon, 29 Jun 2020 22:43:30: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:43:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:43:34: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:43:34: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:43:36: 11000000 INFO @ Mon, 29 Jun 2020 22:43:37: 5000000 INFO @ Mon, 29 Jun 2020 22:43:40: 1000000 INFO @ Mon, 29 Jun 2020 22:43:42: 12000000 INFO @ Mon, 29 Jun 2020 22:43:43: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:43:43: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:43:43: #1 total tags in treatment: 12224613 INFO @ Mon, 29 Jun 2020 22:43:43: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:43:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:43:43: #1 tags after filtering in treatment: 12224613 INFO @ Mon, 29 Jun 2020 22:43:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:43:43: #1 finished! INFO @ Mon, 29 Jun 2020 22:43:43: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:43:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:43:44: 6000000 INFO @ Mon, 29 Jun 2020 22:43:44: #2 number of paired peaks: 24 WARNING @ Mon, 29 Jun 2020 22:43:44: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:43:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:43:46: 2000000 INFO @ Mon, 29 Jun 2020 22:43:50: 7000000 INFO @ Mon, 29 Jun 2020 22:43:52: 3000000 INFO @ Mon, 29 Jun 2020 22:43:57: 8000000 INFO @ Mon, 29 Jun 2020 22:43:57: 4000000 INFO @ Mon, 29 Jun 2020 22:44:03: 5000000 INFO @ Mon, 29 Jun 2020 22:44:03: 9000000 INFO @ Mon, 29 Jun 2020 22:44:09: 6000000 INFO @ Mon, 29 Jun 2020 22:44:10: 10000000 INFO @ Mon, 29 Jun 2020 22:44:15: 7000000 INFO @ Mon, 29 Jun 2020 22:44:16: 11000000 INFO @ Mon, 29 Jun 2020 22:44:21: 8000000 INFO @ Mon, 29 Jun 2020 22:44:23: 12000000 INFO @ Mon, 29 Jun 2020 22:44:24: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:44:24: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:44:24: #1 total tags in treatment: 12224613 INFO @ Mon, 29 Jun 2020 22:44:24: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:44:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:44:25: #1 tags after filtering in treatment: 12224613 INFO @ Mon, 29 Jun 2020 22:44:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:44:25: #1 finished! INFO @ Mon, 29 Jun 2020 22:44:25: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:44:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:44:25: #2 number of paired peaks: 24 WARNING @ Mon, 29 Jun 2020 22:44:25: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:44:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:44:26: 9000000 INFO @ Mon, 29 Jun 2020 22:44:32: 10000000 INFO @ Mon, 29 Jun 2020 22:44:37: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:44:43: 12000000 INFO @ Mon, 29 Jun 2020 22:44:44: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:44:44: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:44:44: #1 total tags in treatment: 12224613 INFO @ Mon, 29 Jun 2020 22:44:44: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:44:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:44:45: #1 tags after filtering in treatment: 12224613 INFO @ Mon, 29 Jun 2020 22:44:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:44:45: #1 finished! INFO @ Mon, 29 Jun 2020 22:44:45: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:44:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:44:45: #2 number of paired peaks: 24 WARNING @ Mon, 29 Jun 2020 22:44:45: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:44:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287827/SRX287827.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。