Job ID = 6527831 SRX = SRX287803 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:53:50 prefetch.2.10.7: 1) Downloading 'SRR869992'... 2020-06-29T13:53:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:56:33 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:56:33 prefetch.2.10.7: 1) 'SRR869992' was downloaded successfully Read 20657054 spots for SRR869992/SRR869992.sra Written 20657054 spots for SRR869992/SRR869992.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:21 20657054 reads; of these: 20657054 (100.00%) were unpaired; of these: 1440416 (6.97%) aligned 0 times 14249864 (68.98%) aligned exactly 1 time 4966774 (24.04%) aligned >1 times 93.03% overall alignment rate Time searching: 00:06:23 Overall time: 00:06:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4461068 / 19216638 = 0.2321 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:14:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:14:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:14:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:14:22: 1000000 INFO @ Mon, 29 Jun 2020 23:14:28: 2000000 INFO @ Mon, 29 Jun 2020 23:14:34: 3000000 INFO @ Mon, 29 Jun 2020 23:14:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:14:45: 5000000 INFO @ Mon, 29 Jun 2020 23:14:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:14:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:14:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:14:51: 6000000 INFO @ Mon, 29 Jun 2020 23:14:53: 1000000 INFO @ Mon, 29 Jun 2020 23:14:58: 7000000 INFO @ Mon, 29 Jun 2020 23:14:59: 2000000 INFO @ Mon, 29 Jun 2020 23:15:04: 8000000 INFO @ Mon, 29 Jun 2020 23:15:05: 3000000 INFO @ Mon, 29 Jun 2020 23:15:10: 9000000 INFO @ Mon, 29 Jun 2020 23:15:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:15:16: 10000000 INFO @ Mon, 29 Jun 2020 23:15:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:15:16: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:15:16: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:15:18: 5000000 INFO @ Mon, 29 Jun 2020 23:15:22: 11000000 INFO @ Mon, 29 Jun 2020 23:15:23: 1000000 INFO @ Mon, 29 Jun 2020 23:15:24: 6000000 INFO @ Mon, 29 Jun 2020 23:15:29: 12000000 INFO @ Mon, 29 Jun 2020 23:15:29: 2000000 INFO @ Mon, 29 Jun 2020 23:15:30: 7000000 INFO @ Mon, 29 Jun 2020 23:15:35: 13000000 INFO @ Mon, 29 Jun 2020 23:15:36: 3000000 INFO @ Mon, 29 Jun 2020 23:15:37: 8000000 INFO @ Mon, 29 Jun 2020 23:15:42: 14000000 INFO @ Mon, 29 Jun 2020 23:15:42: 4000000 INFO @ Mon, 29 Jun 2020 23:15:43: 9000000 INFO @ Mon, 29 Jun 2020 23:15:46: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:15:46: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:15:46: #1 total tags in treatment: 14755570 INFO @ Mon, 29 Jun 2020 23:15:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:15:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:15:47: #1 tags after filtering in treatment: 14755570 INFO @ Mon, 29 Jun 2020 23:15:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:15:47: #1 finished! INFO @ Mon, 29 Jun 2020 23:15:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:15:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:15:48: #2 number of paired peaks: 11 WARNING @ Mon, 29 Jun 2020 23:15:48: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:15:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:15:48: 5000000 INFO @ Mon, 29 Jun 2020 23:15:49: 10000000 INFO @ Mon, 29 Jun 2020 23:15:54: 6000000 INFO @ Mon, 29 Jun 2020 23:15:56: 11000000 INFO @ Mon, 29 Jun 2020 23:16:00: 7000000 INFO @ Mon, 29 Jun 2020 23:16:02: 12000000 INFO @ Mon, 29 Jun 2020 23:16:07: 8000000 INFO @ Mon, 29 Jun 2020 23:16:08: 13000000 INFO @ Mon, 29 Jun 2020 23:16:13: 9000000 INFO @ Mon, 29 Jun 2020 23:16:14: 14000000 INFO @ Mon, 29 Jun 2020 23:16:19: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:16:19: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:16:19: #1 total tags in treatment: 14755570 INFO @ Mon, 29 Jun 2020 23:16:19: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:16:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:16:19: 10000000 INFO @ Mon, 29 Jun 2020 23:16:19: #1 tags after filtering in treatment: 14755570 INFO @ Mon, 29 Jun 2020 23:16:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:16:19: #1 finished! INFO @ Mon, 29 Jun 2020 23:16:19: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:16:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:16:20: #2 number of paired peaks: 11 WARNING @ Mon, 29 Jun 2020 23:16:20: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:16:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:16:25: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:16:31: 12000000 INFO @ Mon, 29 Jun 2020 23:16:37: 13000000 INFO @ Mon, 29 Jun 2020 23:16:42: 14000000 INFO @ Mon, 29 Jun 2020 23:16:47: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:16:47: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:16:47: #1 total tags in treatment: 14755570 INFO @ Mon, 29 Jun 2020 23:16:47: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:16:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:16:47: #1 tags after filtering in treatment: 14755570 INFO @ Mon, 29 Jun 2020 23:16:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:16:47: #1 finished! INFO @ Mon, 29 Jun 2020 23:16:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:16:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:16:48: #2 number of paired peaks: 11 WARNING @ Mon, 29 Jun 2020 23:16:48: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:16:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287803/SRX287803.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。