Job ID = 6527827 SRX = SRX287780 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:35:22 prefetch.2.10.7: 1) Downloading 'SRR869969'... 2020-06-29T13:35:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:40:14 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:40:14 prefetch.2.10.7: 1) 'SRR869969' was downloaded successfully Read 25514408 spots for SRR869969/SRR869969.sra Written 25514408 spots for SRR869969/SRR869969.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:33 25514408 reads; of these: 25514408 (100.00%) were unpaired; of these: 2519321 (9.87%) aligned 0 times 18126671 (71.04%) aligned exactly 1 time 4868416 (19.08%) aligned >1 times 90.13% overall alignment rate Time searching: 00:08:33 Overall time: 00:08:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3709658 / 22995087 = 0.1613 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:01:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:01:42: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:01:42: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:01:48: 1000000 INFO @ Mon, 29 Jun 2020 23:01:54: 2000000 INFO @ Mon, 29 Jun 2020 23:02:00: 3000000 INFO @ Mon, 29 Jun 2020 23:02:05: 4000000 INFO @ Mon, 29 Jun 2020 23:02:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:02:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:02:12: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:02:12: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:02:16: 6000000 INFO @ Mon, 29 Jun 2020 23:02:18: 1000000 INFO @ Mon, 29 Jun 2020 23:02:22: 7000000 INFO @ Mon, 29 Jun 2020 23:02:24: 2000000 INFO @ Mon, 29 Jun 2020 23:02:28: 8000000 INFO @ Mon, 29 Jun 2020 23:02:30: 3000000 INFO @ Mon, 29 Jun 2020 23:02:34: 9000000 INFO @ Mon, 29 Jun 2020 23:02:35: 4000000 INFO @ Mon, 29 Jun 2020 23:02:40: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:02:41: 5000000 INFO @ Mon, 29 Jun 2020 23:02:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:02:42: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:02:42: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:02:46: 11000000 INFO @ Mon, 29 Jun 2020 23:02:47: 6000000 INFO @ Mon, 29 Jun 2020 23:02:49: 1000000 INFO @ Mon, 29 Jun 2020 23:02:52: 12000000 INFO @ Mon, 29 Jun 2020 23:02:53: 7000000 INFO @ Mon, 29 Jun 2020 23:02:56: 2000000 INFO @ Mon, 29 Jun 2020 23:02:58: 13000000 INFO @ Mon, 29 Jun 2020 23:02:59: 8000000 INFO @ Mon, 29 Jun 2020 23:03:03: 3000000 INFO @ Mon, 29 Jun 2020 23:03:05: 14000000 INFO @ Mon, 29 Jun 2020 23:03:05: 9000000 INFO @ Mon, 29 Jun 2020 23:03:09: 4000000 INFO @ Mon, 29 Jun 2020 23:03:11: 15000000 INFO @ Mon, 29 Jun 2020 23:03:11: 10000000 INFO @ Mon, 29 Jun 2020 23:03:16: 5000000 INFO @ Mon, 29 Jun 2020 23:03:17: 11000000 INFO @ Mon, 29 Jun 2020 23:03:17: 16000000 INFO @ Mon, 29 Jun 2020 23:03:23: 6000000 INFO @ Mon, 29 Jun 2020 23:03:23: 12000000 INFO @ Mon, 29 Jun 2020 23:03:23: 17000000 INFO @ Mon, 29 Jun 2020 23:03:29: 13000000 INFO @ Mon, 29 Jun 2020 23:03:29: 7000000 INFO @ Mon, 29 Jun 2020 23:03:30: 18000000 INFO @ Mon, 29 Jun 2020 23:03:35: 14000000 INFO @ Mon, 29 Jun 2020 23:03:36: 19000000 INFO @ Mon, 29 Jun 2020 23:03:36: 8000000 INFO @ Mon, 29 Jun 2020 23:03:38: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:03:38: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:03:38: #1 total tags in treatment: 19285429 INFO @ Mon, 29 Jun 2020 23:03:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:03:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:03:38: #1 tags after filtering in treatment: 19285429 INFO @ Mon, 29 Jun 2020 23:03:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:03:38: #1 finished! INFO @ Mon, 29 Jun 2020 23:03:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:03:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:03:39: #2 number of paired peaks: 20 WARNING @ Mon, 29 Jun 2020 23:03:39: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:03:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:03:41: 15000000 INFO @ Mon, 29 Jun 2020 23:03:43: 9000000 INFO @ Mon, 29 Jun 2020 23:03:47: 16000000 INFO @ Mon, 29 Jun 2020 23:03:49: 10000000 INFO @ Mon, 29 Jun 2020 23:03:53: 17000000 INFO @ Mon, 29 Jun 2020 23:03:56: 11000000 INFO @ Mon, 29 Jun 2020 23:03:59: 18000000 INFO @ Mon, 29 Jun 2020 23:04:03: 12000000 INFO @ Mon, 29 Jun 2020 23:04:05: 19000000 INFO @ Mon, 29 Jun 2020 23:04:07: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:04:07: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:04:07: #1 total tags in treatment: 19285429 INFO @ Mon, 29 Jun 2020 23:04:07: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:04:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:04:07: #1 tags after filtering in treatment: 19285429 INFO @ Mon, 29 Jun 2020 23:04:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:04:07: #1 finished! INFO @ Mon, 29 Jun 2020 23:04:07: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:04:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:04:09: #2 number of paired peaks: 20 WARNING @ Mon, 29 Jun 2020 23:04:09: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:04:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:04:09: 13000000 INFO @ Mon, 29 Jun 2020 23:04:16: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:04:22: 15000000 INFO @ Mon, 29 Jun 2020 23:04:28: 16000000 INFO @ Mon, 29 Jun 2020 23:04:35: 17000000 INFO @ Mon, 29 Jun 2020 23:04:41: 18000000 INFO @ Mon, 29 Jun 2020 23:04:47: 19000000 INFO @ Mon, 29 Jun 2020 23:04:49: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:04:49: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:04:49: #1 total tags in treatment: 19285429 INFO @ Mon, 29 Jun 2020 23:04:49: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:04:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:04:49: #1 tags after filtering in treatment: 19285429 INFO @ Mon, 29 Jun 2020 23:04:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:04:49: #1 finished! INFO @ Mon, 29 Jun 2020 23:04:49: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:04:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:04:51: #2 number of paired peaks: 20 WARNING @ Mon, 29 Jun 2020 23:04:51: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:04:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287780/SRX287780.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。