Job ID = 6527825 SRX = SRX287777 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:28:37 prefetch.2.10.7: 1) Downloading 'SRR869966'... 2020-06-29T13:28:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:31:17 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:31:17 prefetch.2.10.7: 1) 'SRR869966' was downloaded successfully Read 17761171 spots for SRR869966/SRR869966.sra Written 17761171 spots for SRR869966/SRR869966.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:47 17761171 reads; of these: 17761171 (100.00%) were unpaired; of these: 964294 (5.43%) aligned 0 times 12930957 (72.80%) aligned exactly 1 time 3865920 (21.77%) aligned >1 times 94.57% overall alignment rate Time searching: 00:05:47 Overall time: 00:05:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1267252 / 16796877 = 0.0754 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:47:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:47:16: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:47:16: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:47:22: 1000000 INFO @ Mon, 29 Jun 2020 22:47:27: 2000000 INFO @ Mon, 29 Jun 2020 22:47:33: 3000000 INFO @ Mon, 29 Jun 2020 22:47:38: 4000000 INFO @ Mon, 29 Jun 2020 22:47:43: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:47:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:47:46: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:47:46: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:47:49: 6000000 INFO @ Mon, 29 Jun 2020 22:47:52: 1000000 INFO @ Mon, 29 Jun 2020 22:47:54: 7000000 INFO @ Mon, 29 Jun 2020 22:47:57: 2000000 INFO @ Mon, 29 Jun 2020 22:48:00: 8000000 INFO @ Mon, 29 Jun 2020 22:48:02: 3000000 INFO @ Mon, 29 Jun 2020 22:48:05: 9000000 INFO @ Mon, 29 Jun 2020 22:48:07: 4000000 INFO @ Mon, 29 Jun 2020 22:48:11: 10000000 INFO @ Mon, 29 Jun 2020 22:48:12: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:48:16: 11000000 INFO @ Mon, 29 Jun 2020 22:48:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:48:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:48:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:48:17: 6000000 INFO @ Mon, 29 Jun 2020 22:48:22: 12000000 INFO @ Mon, 29 Jun 2020 22:48:22: 1000000 INFO @ Mon, 29 Jun 2020 22:48:22: 7000000 INFO @ Mon, 29 Jun 2020 22:48:27: 2000000 INFO @ Mon, 29 Jun 2020 22:48:27: 13000000 INFO @ Mon, 29 Jun 2020 22:48:27: 8000000 INFO @ Mon, 29 Jun 2020 22:48:32: 3000000 INFO @ Mon, 29 Jun 2020 22:48:32: 9000000 INFO @ Mon, 29 Jun 2020 22:48:33: 14000000 INFO @ Mon, 29 Jun 2020 22:48:37: 4000000 INFO @ Mon, 29 Jun 2020 22:48:37: 10000000 INFO @ Mon, 29 Jun 2020 22:48:38: 15000000 INFO @ Mon, 29 Jun 2020 22:48:41: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:48:41: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:48:41: #1 total tags in treatment: 15529625 INFO @ Mon, 29 Jun 2020 22:48:41: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:48:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:48:41: #1 tags after filtering in treatment: 15529625 INFO @ Mon, 29 Jun 2020 22:48:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:48:41: #1 finished! INFO @ Mon, 29 Jun 2020 22:48:41: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:48:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:48:42: 5000000 INFO @ Mon, 29 Jun 2020 22:48:42: 11000000 INFO @ Mon, 29 Jun 2020 22:48:42: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:48:42: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:48:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:48:47: 12000000 INFO @ Mon, 29 Jun 2020 22:48:47: 6000000 INFO @ Mon, 29 Jun 2020 22:48:52: 13000000 INFO @ Mon, 29 Jun 2020 22:48:52: 7000000 INFO @ Mon, 29 Jun 2020 22:48:57: 14000000 INFO @ Mon, 29 Jun 2020 22:48:57: 8000000 INFO @ Mon, 29 Jun 2020 22:49:02: 9000000 INFO @ Mon, 29 Jun 2020 22:49:02: 15000000 INFO @ Mon, 29 Jun 2020 22:49:05: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:49:05: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:49:05: #1 total tags in treatment: 15529625 INFO @ Mon, 29 Jun 2020 22:49:05: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:49:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:49:05: #1 tags after filtering in treatment: 15529625 INFO @ Mon, 29 Jun 2020 22:49:05: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:49:05: #1 finished! INFO @ Mon, 29 Jun 2020 22:49:05: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:49:05: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:49:06: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:49:06: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:49:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:49:07: 10000000 INFO @ Mon, 29 Jun 2020 22:49:12: 11000000 INFO @ Mon, 29 Jun 2020 22:49:17: 12000000 INFO @ Mon, 29 Jun 2020 22:49:22: 13000000 INFO @ Mon, 29 Jun 2020 22:49:27: 14000000 INFO @ Mon, 29 Jun 2020 22:49:32: 15000000 INFO @ Mon, 29 Jun 2020 22:49:34: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 22:49:34: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 22:49:34: #1 total tags in treatment: 15529625 INFO @ Mon, 29 Jun 2020 22:49:34: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:49:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:49:35: #1 tags after filtering in treatment: 15529625 INFO @ Mon, 29 Jun 2020 22:49:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:49:35: #1 finished! INFO @ Mon, 29 Jun 2020 22:49:35: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:49:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:49:36: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:49:36: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:49:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX287777/SRX287777.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。